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Inhibitory effect of gallic acid on CCl4-mediated liver fibrosis in mice.
Cell Biochem Biophys. 2014 May; 69(1):21-6.CB

Abstract

The aim of this study was to investigate the effect of gallic acid (GA) on liver fibrosis induced by carbon tetrachloride (CCl4). Male BALB/c mice were randomly divided into four groups: normal control group (group A), CCl4-induced liver injury control group (group B), and CCl4 induction with GA of low dose (5 mg/kg) and high dose (15 mg/kg) treatment group (group C and group D). GA was intra-gastric given for mice once a day after 2 weeks of CCl4 induction. Animals were killed at the eighth week. Degrees of fibrosis and collagen percentage were measured. Hyaluronic acid (HA), type IV collagen (cIV), malondialdehyde (MDA), alanine aminotransferase (ALT), aspartate aminotransferase (AST), and gamma-glutamyl transferase (γ-GT) were determined. Expression of matrix metalloproteinases-2 (MMP-2) and tissue inhibitor of matrix metalloproteinases-1 (TIMP-1) mRNA levels were examined by RT-PCR. Western blotting was carried out to evaluate the changes of MMP-2 protein. HE and VG stainings showed GA in a dose-dependent manner improved significantly the fibrosis condition in CCl4-injured mice (P < 0.05 or P < 0.01). Also, the concentrations of HA, cIV, and MDA, as well as the serum levels of ALT, AST, and γ-GT were markedly reduced by GA (P < 0.05 or P < 0.01), and decreases in MMP-2, TIMP-1 mRNA, and MMP-2 protein were observed as well (P < 0.05 or P < 0.01). GA could exert protective effect on liver injury and reduce liver fibrosis induced by CCl4 in mice, which might be through the inhibition of hepatic stellate cell activity.

Authors+Show Affiliations

School of Life Sciences, Jilin Agricultural University, Changchun, 130118, China.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

24096707

Citation

Wang, Jing, et al. "Inhibitory Effect of Gallic Acid On CCl4-mediated Liver Fibrosis in Mice." Cell Biochemistry and Biophysics, vol. 69, no. 1, 2014, pp. 21-6.
Wang J, Tang L, White J, et al. Inhibitory effect of gallic acid on CCl4-mediated liver fibrosis in mice. Cell Biochem Biophys. 2014;69(1):21-6.
Wang, J., Tang, L., White, J., & Fang, J. (2014). Inhibitory effect of gallic acid on CCl4-mediated liver fibrosis in mice. Cell Biochemistry and Biophysics, 69(1), 21-6. https://doi.org/10.1007/s12013-013-9761-y
Wang J, et al. Inhibitory Effect of Gallic Acid On CCl4-mediated Liver Fibrosis in Mice. Cell Biochem Biophys. 2014;69(1):21-6. PubMed PMID: 24096707.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Inhibitory effect of gallic acid on CCl4-mediated liver fibrosis in mice. AU - Wang,Jing, AU - Tang,Long, AU - White,James, AU - Fang,Jing, PY - 2013/10/8/entrez PY - 2013/10/8/pubmed PY - 2014/12/15/medline SP - 21 EP - 6 JF - Cell biochemistry and biophysics JO - Cell Biochem Biophys VL - 69 IS - 1 N2 - The aim of this study was to investigate the effect of gallic acid (GA) on liver fibrosis induced by carbon tetrachloride (CCl4). Male BALB/c mice were randomly divided into four groups: normal control group (group A), CCl4-induced liver injury control group (group B), and CCl4 induction with GA of low dose (5 mg/kg) and high dose (15 mg/kg) treatment group (group C and group D). GA was intra-gastric given for mice once a day after 2 weeks of CCl4 induction. Animals were killed at the eighth week. Degrees of fibrosis and collagen percentage were measured. Hyaluronic acid (HA), type IV collagen (cIV), malondialdehyde (MDA), alanine aminotransferase (ALT), aspartate aminotransferase (AST), and gamma-glutamyl transferase (γ-GT) were determined. Expression of matrix metalloproteinases-2 (MMP-2) and tissue inhibitor of matrix metalloproteinases-1 (TIMP-1) mRNA levels were examined by RT-PCR. Western blotting was carried out to evaluate the changes of MMP-2 protein. HE and VG stainings showed GA in a dose-dependent manner improved significantly the fibrosis condition in CCl4-injured mice (P < 0.05 or P < 0.01). Also, the concentrations of HA, cIV, and MDA, as well as the serum levels of ALT, AST, and γ-GT were markedly reduced by GA (P < 0.05 or P < 0.01), and decreases in MMP-2, TIMP-1 mRNA, and MMP-2 protein were observed as well (P < 0.05 or P < 0.01). GA could exert protective effect on liver injury and reduce liver fibrosis induced by CCl4 in mice, which might be through the inhibition of hepatic stellate cell activity. SN - 1559-0283 UR - https://www.unboundmedicine.com/medline/citation/24096707/Inhibitory_effect_of_gallic_acid_on_CCl4_mediated_liver_fibrosis_in_mice_ DB - PRIME DP - Unbound Medicine ER -