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Hepatoprotective role of Nicotiana plumbaginifolia Linn. against carbon tetrachloride-induced injuries.
Toxicol Ind Health. 2016 Feb; 32(2):292-8.TI

Abstract

Nicotiana plumbignifolia (Linn) is used as folk medicine in the treatment of liver dysfunction in Pakistan. The present study was designed to investigate the hepatoprotective role of N. plumbignifolia methanolice extract (NPME) against carbon tetrachloride (CCl4)-induced oxidative damage in liver of chicks. Methanolic extract of N. plumbignifolia was obtained and was further evaluated as a hepatoprotective agent against CCl4-induced oxidative damage in liver of chicks. For this study, 60-day-old 50 male chicks were divided into five groups. Chicks of group 1 (control) had free access to food and water. Group II received 1 mL/kg of CCl4 (30% in olive oil v/v) via the intraperitoneal route thrice a week for 4 weeks. Group III received 100 mg/kg body weight (b.w.) of silymarin via gavage after 48 h of CCl4 treatment, whereas group IV were given 200 mg/kg b.w. NPME after 48 h of CCl4 treatment. Hepatoprotective activity was assessed by measuring the activities of the antioxidant enzymes: catalase, peroxidase, superoxide dismutase, glutathione peroxidase, glutathione reductase, and lipid peroxidation (thiobarbituric acid reactive substances (TBARS)). Serum was analyzed for various biochemical parameters. The results revealed that CCl4 induced oxidative stress as evidenced by the significant decrease in the activity levels of antioxidant enzymes, while an increase in the levels of TBARS in liver samples is compared with the control group. Serum levels lactate dehydrogenase, triglycerides, total cholesterol, and low-density lipoprotein was elevated while reducing high-density lipoprotein compared to controls. Cotreatment of NPME treatment reversed these alterations, which seems likely that NPME can protect the liver tissues against CCl4-mediated oxidative damage.

Authors+Show Affiliations

Department of Biotechnology, Faculty of Biological Sciences, University of Science and Technology, Khyber Pakhtunkhwa, Pakistan.Department of Biotechnology, Faculty of Biological Sciences, University of Science and Technology, Khyber Pakhtunkhwa, Pakistan Rahmatgul_81@yahoo.com.Department of Biotechnology, Faculty of Biological Sciences, University of Science and Technology, Khyber Pakhtunkhwa, Pakistan.Department of Biotechnology, Faculty of Biological Sciences, University of Science and Technology, Khyber Pakhtunkhwa, Pakistan.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

24097354

Citation

Shah, Abdus Saboor, et al. "Hepatoprotective Role of Nicotiana Plumbaginifolia Linn. Against Carbon Tetrachloride-induced Injuries." Toxicology and Industrial Health, vol. 32, no. 2, 2016, pp. 292-8.
Shah AS, Khan RA, Ahmed M, et al. Hepatoprotective role of Nicotiana plumbaginifolia Linn. against carbon tetrachloride-induced injuries. Toxicol Ind Health. 2016;32(2):292-8.
Shah, A. S., Khan, R. A., Ahmed, M., & Muhammad, N. (2016). Hepatoprotective role of Nicotiana plumbaginifolia Linn. against carbon tetrachloride-induced injuries. Toxicology and Industrial Health, 32(2), 292-8. https://doi.org/10.1177/0748233713498448
Shah AS, et al. Hepatoprotective Role of Nicotiana Plumbaginifolia Linn. Against Carbon Tetrachloride-induced Injuries. Toxicol Ind Health. 2016;32(2):292-8. PubMed PMID: 24097354.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Hepatoprotective role of Nicotiana plumbaginifolia Linn. against carbon tetrachloride-induced injuries. AU - Shah,Abdus Saboor, AU - Khan,Rahmat Ali, AU - Ahmed,Mushtaq, AU - Muhammad,Nawshad, Y1 - 2013/10/04/ PY - 2013/10/8/entrez PY - 2013/10/8/pubmed PY - 2016/10/26/medline KW - CCl4 KW - Hepatoprotective KW - Nicotiana plumbignifolia KW - oxidative stress KW - silymarin SP - 292 EP - 8 JF - Toxicology and industrial health JO - Toxicol Ind Health VL - 32 IS - 2 N2 - Nicotiana plumbignifolia (Linn) is used as folk medicine in the treatment of liver dysfunction in Pakistan. The present study was designed to investigate the hepatoprotective role of N. plumbignifolia methanolice extract (NPME) against carbon tetrachloride (CCl4)-induced oxidative damage in liver of chicks. Methanolic extract of N. plumbignifolia was obtained and was further evaluated as a hepatoprotective agent against CCl4-induced oxidative damage in liver of chicks. For this study, 60-day-old 50 male chicks were divided into five groups. Chicks of group 1 (control) had free access to food and water. Group II received 1 mL/kg of CCl4 (30% in olive oil v/v) via the intraperitoneal route thrice a week for 4 weeks. Group III received 100 mg/kg body weight (b.w.) of silymarin via gavage after 48 h of CCl4 treatment, whereas group IV were given 200 mg/kg b.w. NPME after 48 h of CCl4 treatment. Hepatoprotective activity was assessed by measuring the activities of the antioxidant enzymes: catalase, peroxidase, superoxide dismutase, glutathione peroxidase, glutathione reductase, and lipid peroxidation (thiobarbituric acid reactive substances (TBARS)). Serum was analyzed for various biochemical parameters. The results revealed that CCl4 induced oxidative stress as evidenced by the significant decrease in the activity levels of antioxidant enzymes, while an increase in the levels of TBARS in liver samples is compared with the control group. Serum levels lactate dehydrogenase, triglycerides, total cholesterol, and low-density lipoprotein was elevated while reducing high-density lipoprotein compared to controls. Cotreatment of NPME treatment reversed these alterations, which seems likely that NPME can protect the liver tissues against CCl4-mediated oxidative damage. SN - 1477-0393 UR - https://www.unboundmedicine.com/medline/citation/24097354/Hepatoprotective_role_of_Nicotiana_plumbaginifolia_Linn__against_carbon_tetrachloride_induced_injuries_ L2 - http://journals.sagepub.com/doi/full/10.1177/0748233713498448?url_ver=Z39.88-2003&rfr_id=ori:rid:crossref.org&rfr_dat=cr_pub=pubmed DB - PRIME DP - Unbound Medicine ER -