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Antimicrobial constituents of the leaves of Mikania micrantha H. B. K.
PLoS One. 2013; 8(10):e76725.Plos

Abstract

BACKGROUND

To isolate plant-derived compounds with antimicrobial activity from the leaves of Mikania micrantha, to determine the compounds configuration, and to evaluate their antimicrobial activity against eight plant pathogenic fungi (Exserohilum turcicum, Colletotrichum lagenarium, Pseudoperonispora cubensis, Botrytis cirerea, Rhizoctonia solani, Phytophthora parasitica, Fusarium solani, and Pythium aphanidermatum,) and four plant pathogenic bacteria (gram negative bacteria: Ralstonia dolaanacearum, Xanthomonas oryzae pv. Oryzae, Xanthomonas Campestris pv. Vesicatoria, and Xanthomonas campestris pv. Citri), and four bacteria (gram positive bacteria: Staphyloccocus aureus, Bacillus subtilis, Micrococcus luteus, and Bacillus cereus).

METHODS AND RESULTS

Antimicrobial constituents of the leaves of M. micrantha were isolated using bioactivity- guided fractionation. The antifungal activity of the isolated compounds was evaluated by the inhibit hypha growth method and inhibit spore germination method. Characterization of antibacterial activity was carried out using the minimum inhibitory concentrations (MICs) and the minimum bactericidal concentrations (MBCs). MIC and MBC were determined by the broth microdilution method. Six compounds - deoxymikanolide, scandenolide, dihydroscandenolide, mikanolide, dihydromikanolide, and m - methoxy benzoic acid - have been isolated from leaves of Mikania micrantha H. B. K. Deoxymikanolide, scandenolide, and dihydroscandenolide were new compounds. The result of bioassay showed that all of isolated compounds were effective against tested strains and deoxymikanolide showed the strongest activity.

CONCLUSIONS AND SIGNIFICANCE

The leaves of M. micrantha may be a promising source in the search for new antimicrobial drugs due to its efficacy and the broadest range. Meanwhile, adverse impact of M. micrantha will be eliminated.

Authors+Show Affiliations

Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing, P. R. China.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

24098556

Citation

Li, Yan, et al. "Antimicrobial Constituents of the Leaves of Mikania Micrantha H. B. K." PloS One, vol. 8, no. 10, 2013, pp. e76725.
Li Y, Li J, Li Y, et al. Antimicrobial constituents of the leaves of Mikania micrantha H. B. K. PLoS ONE. 2013;8(10):e76725.
Li, Y., Li, J., Li, Y., Wang, X. X., & Cao, A. C. (2013). Antimicrobial constituents of the leaves of Mikania micrantha H. B. K. PloS One, 8(10), e76725. https://doi.org/10.1371/journal.pone.0076725
Li Y, et al. Antimicrobial Constituents of the Leaves of Mikania Micrantha H. B. K. PLoS ONE. 2013;8(10):e76725. PubMed PMID: 24098556.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Antimicrobial constituents of the leaves of Mikania micrantha H. B. K. AU - Li,Yan, AU - Li,Jun, AU - Li,Yuan, AU - Wang,Xia-xia, AU - Cao,Ao-cheng, Y1 - 2013/10/02/ PY - 2013/06/17/received PY - 2013/08/19/accepted PY - 2013/10/8/entrez PY - 2013/10/8/pubmed PY - 2014/4/30/medline SP - e76725 EP - e76725 JF - PloS one JO - PLoS ONE VL - 8 IS - 10 N2 - BACKGROUND: To isolate plant-derived compounds with antimicrobial activity from the leaves of Mikania micrantha, to determine the compounds configuration, and to evaluate their antimicrobial activity against eight plant pathogenic fungi (Exserohilum turcicum, Colletotrichum lagenarium, Pseudoperonispora cubensis, Botrytis cirerea, Rhizoctonia solani, Phytophthora parasitica, Fusarium solani, and Pythium aphanidermatum,) and four plant pathogenic bacteria (gram negative bacteria: Ralstonia dolaanacearum, Xanthomonas oryzae pv. Oryzae, Xanthomonas Campestris pv. Vesicatoria, and Xanthomonas campestris pv. Citri), and four bacteria (gram positive bacteria: Staphyloccocus aureus, Bacillus subtilis, Micrococcus luteus, and Bacillus cereus). METHODS AND RESULTS: Antimicrobial constituents of the leaves of M. micrantha were isolated using bioactivity- guided fractionation. The antifungal activity of the isolated compounds was evaluated by the inhibit hypha growth method and inhibit spore germination method. Characterization of antibacterial activity was carried out using the minimum inhibitory concentrations (MICs) and the minimum bactericidal concentrations (MBCs). MIC and MBC were determined by the broth microdilution method. Six compounds - deoxymikanolide, scandenolide, dihydroscandenolide, mikanolide, dihydromikanolide, and m - methoxy benzoic acid - have been isolated from leaves of Mikania micrantha H. B. K. Deoxymikanolide, scandenolide, and dihydroscandenolide were new compounds. The result of bioassay showed that all of isolated compounds were effective against tested strains and deoxymikanolide showed the strongest activity. CONCLUSIONS AND SIGNIFICANCE: The leaves of M. micrantha may be a promising source in the search for new antimicrobial drugs due to its efficacy and the broadest range. Meanwhile, adverse impact of M. micrantha will be eliminated. SN - 1932-6203 UR - https://www.unboundmedicine.com/medline/citation/24098556/Antimicrobial_constituents_of_the_leaves_of_Mikania_micrantha_H__B__K_ L2 - http://dx.plos.org/10.1371/journal.pone.0076725 DB - PRIME DP - Unbound Medicine ER -