Tags

Type your tag names separated by a space and hit enter

Phanta: a non-fluorescent photochromic acceptor for pcFRET.
PLoS One 2013; 8(9):e75835Plos

Abstract

We have developed an orange non-fluorescent photochromic protein (quantum yield, 0.003) we call Phanta that is useful as an acceptor in pcFRET applications. Phanta can be repeatedly inter-converted between the two absorbing states by alternate exposure to cyan and violet light. The absorption spectra of Phanta in one absorbing state shows excellent overlap with the emission spectra of a number of donor green fluorescent proteins including the commonly used EGFP. We show that the Phanta-EGFP FRET pair is suitable for monitoring the activation of caspase 3 in live cells using readily available instrumentation and a simple protocol that requires the acquisition of two donor emission images corresponding to Phanta in each of its photoswitched states. This the first report of a genetically encoded non-fluorescent acceptor for pcFRET.

Authors+Show Affiliations

Department of Biochemistry and Molecular Biology, Monash University, Clayton Campus, Melbourne, Victoria, Australia.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

24098733

Citation

Don Paul, Craig, et al. "Phanta: a Non-fluorescent Photochromic Acceptor for PcFRET." PloS One, vol. 8, no. 9, 2013, pp. e75835.
Don Paul C, Kiss C, Traore DA, et al. Phanta: a non-fluorescent photochromic acceptor for pcFRET. PLoS ONE. 2013;8(9):e75835.
Don Paul, C., Kiss, C., Traore, D. A., Gong, L., Wilce, M. C., Devenish, R. J., ... Prescott, M. (2013). Phanta: a non-fluorescent photochromic acceptor for pcFRET. PloS One, 8(9), pp. e75835. doi:10.1371/journal.pone.0075835.
Don Paul C, et al. Phanta: a Non-fluorescent Photochromic Acceptor for PcFRET. PLoS ONE. 2013;8(9):e75835. PubMed PMID: 24098733.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Phanta: a non-fluorescent photochromic acceptor for pcFRET. AU - Don Paul,Craig, AU - Kiss,Csaba, AU - Traore,Daouda A K, AU - Gong,Lan, AU - Wilce,Matthew C J, AU - Devenish,Rodney J, AU - Bradbury,Andrew, AU - Prescott,Mark, Y1 - 2013/09/30/ PY - 2013/07/17/received PY - 2013/08/22/accepted PY - 2013/10/8/entrez PY - 2013/10/8/pubmed PY - 2014/6/17/medline SP - e75835 EP - e75835 JF - PloS one JO - PLoS ONE VL - 8 IS - 9 N2 - We have developed an orange non-fluorescent photochromic protein (quantum yield, 0.003) we call Phanta that is useful as an acceptor in pcFRET applications. Phanta can be repeatedly inter-converted between the two absorbing states by alternate exposure to cyan and violet light. The absorption spectra of Phanta in one absorbing state shows excellent overlap with the emission spectra of a number of donor green fluorescent proteins including the commonly used EGFP. We show that the Phanta-EGFP FRET pair is suitable for monitoring the activation of caspase 3 in live cells using readily available instrumentation and a simple protocol that requires the acquisition of two donor emission images corresponding to Phanta in each of its photoswitched states. This the first report of a genetically encoded non-fluorescent acceptor for pcFRET. SN - 1932-6203 UR - https://www.unboundmedicine.com/medline/citation/24098733/Phanta:_a_non_fluorescent_photochromic_acceptor_for_pcFRET_ L2 - http://dx.plos.org/10.1371/journal.pone.0075835 DB - PRIME DP - Unbound Medicine ER -