Tags

Type your tag names separated by a space and hit enter

Construction of Co1E1 RNA1 mutants and analysis of their function in vivo.
Basic Life Sci. 1985; 30:321-33.BL

Abstract

We have carried out experiments designed to investigate the relationship between structure and function for the Co1E1 RNA1 species. RNA1 is a small RNA (108 nucleotides) that has been implicated in copy number control of the multicopy plasmid Co1E1. In vitro, RNA1 inhibits the processing of the primer precursor required for initiation of DNA replication. The RNA1 gene is entirely complementary to the 5'-terminal region of the primer. We have functionally separated these 2 RNA species by cloning the RNA1 gene downstream from the S. marcescens trp promoter. When cloned in a Co1E1-compatible plasmid, a trp-RNA1 fusion has been shown to mediate Co1E1-type incompatibility in vivo. The construction scheme described here also generates mutant RNA1 species with altered sequences at the 5' terminus of RNA1 which have been assayed for function in vivo. These experiments have indicated that sequences at the 5' terminus play a critical role in RNA1 function.

Authors

No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, U.S. Gov't, P.H.S.

Language

eng

PubMed ID

2409958

Citation

Polisky, B, et al. "Construction of Co1E1 RNA1 Mutants and Analysis of Their Function in Vivo." Basic Life Sciences, vol. 30, 1985, pp. 321-33.
Polisky B, Tamm J, Fitzwater T. Construction of Co1E1 RNA1 mutants and analysis of their function in vivo. Basic Life Sci. 1985;30:321-33.
Polisky, B., Tamm, J., & Fitzwater, T. (1985). Construction of Co1E1 RNA1 mutants and analysis of their function in vivo. Basic Life Sciences, 30, 321-33.
Polisky B, Tamm J, Fitzwater T. Construction of Co1E1 RNA1 Mutants and Analysis of Their Function in Vivo. Basic Life Sci. 1985;30:321-33. PubMed PMID: 2409958.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Construction of Co1E1 RNA1 mutants and analysis of their function in vivo. AU - Polisky,B, AU - Tamm,J, AU - Fitzwater,T, PY - 1985/1/1/pubmed PY - 1985/1/1/medline PY - 1985/1/1/entrez SP - 321 EP - 33 JF - Basic life sciences JO - Basic Life Sci. VL - 30 N2 - We have carried out experiments designed to investigate the relationship between structure and function for the Co1E1 RNA1 species. RNA1 is a small RNA (108 nucleotides) that has been implicated in copy number control of the multicopy plasmid Co1E1. In vitro, RNA1 inhibits the processing of the primer precursor required for initiation of DNA replication. The RNA1 gene is entirely complementary to the 5'-terminal region of the primer. We have functionally separated these 2 RNA species by cloning the RNA1 gene downstream from the S. marcescens trp promoter. When cloned in a Co1E1-compatible plasmid, a trp-RNA1 fusion has been shown to mediate Co1E1-type incompatibility in vivo. The construction scheme described here also generates mutant RNA1 species with altered sequences at the 5' terminus of RNA1 which have been assayed for function in vivo. These experiments have indicated that sequences at the 5' terminus play a critical role in RNA1 function. SN - 0090-5542 UR - https://www.unboundmedicine.com/medline/citation/2409958/Construction_of_Co1E1_RNA1_mutants_and_analysis_of_their_function_in_vivo_ DB - PRIME DP - Unbound Medicine ER -