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Ectopic expression of a BZR1-1D transcription factor in brassinosteroid signalling enhances carotenoid accumulation and fruit quality attributes in tomato.
Plant Biotechnol J. 2014 Jan; 12(1):105-15.PB

Abstract

The brassinosteroid (BR) response transcription factor Brassinazole resistant 1 (BZR1)-mediated BR signalling regulates many specific developmental processes including fruit ripening. Here, we report the effect of 2,4-epibrassinolide (EBR) and BZR1-1D overexpression on carotenoid accumulation and quality attributes of tomato (Solanum lycopersicum) fruit. EBR-treated pericarp discs of ethylene-insensitive mutant, Never ripe, accumulated significantly more carotenoid than those of the control. The results suggest that BR seems to be involved in modulating pigments accumulation. When three independent transgenic lines overexpressing the Arabidopsis BZR1-1D were used to evaluate the role of BZR1 in regulating tomato fruit carotenoid accumulation and quality attributes, fruits of all three transgenic lines exhibited enhanced carotenoid accumulation and increased soluble solid, soluble sugar and ascorbic acid contents during fruit ripening. In addition, the fruits of two transgenic lines showed dark green shoulder at mature green stage, in accordance with the up-regulated expression level of SlGLK2, which is involved in chloroplast development. Our results indicate the importance of BZR1-centred BR signalling in regulating carotenoid accumulation and quality attributes of tomato fruit and the potential application of the BZR1-like(s) for improvement of nutritional quality and flavour of tomato through genetic engineering.

Authors+Show Affiliations

Department of Horticulture, Key Laboratory of Horticultural Plant Growth, Development and Quality Improvement, Ministry of Agriculture, Zhejiang University, Hangzhou, China.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

24102834

Citation

Liu, Lihong, et al. "Ectopic Expression of a BZR1-1D Transcription Factor in Brassinosteroid Signalling Enhances Carotenoid Accumulation and Fruit Quality Attributes in Tomato." Plant Biotechnology Journal, vol. 12, no. 1, 2014, pp. 105-15.
Liu L, Jia C, Zhang M, et al. Ectopic expression of a BZR1-1D transcription factor in brassinosteroid signalling enhances carotenoid accumulation and fruit quality attributes in tomato. Plant Biotechnol J. 2014;12(1):105-15.
Liu, L., Jia, C., Zhang, M., Chen, D., Chen, S., Guo, R., Guo, D., & Wang, Q. (2014). Ectopic expression of a BZR1-1D transcription factor in brassinosteroid signalling enhances carotenoid accumulation and fruit quality attributes in tomato. Plant Biotechnology Journal, 12(1), 105-15. https://doi.org/10.1111/pbi.12121
Liu L, et al. Ectopic Expression of a BZR1-1D Transcription Factor in Brassinosteroid Signalling Enhances Carotenoid Accumulation and Fruit Quality Attributes in Tomato. Plant Biotechnol J. 2014;12(1):105-15. PubMed PMID: 24102834.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Ectopic expression of a BZR1-1D transcription factor in brassinosteroid signalling enhances carotenoid accumulation and fruit quality attributes in tomato. AU - Liu,Lihong, AU - Jia,Chengguo, AU - Zhang,Min, AU - Chen,Delong, AU - Chen,Sixue, AU - Guo,Rongfang, AU - Guo,Deping, AU - Wang,Qiaomei, Y1 - 2013/09/18/ PY - 2013/03/11/received PY - 2013/08/08/revised PY - 2013/08/12/accepted PY - 2013/10/10/entrez PY - 2013/10/10/pubmed PY - 2014/9/4/medline KW - BZR1 KW - Solanum lycopersicum (tomato) KW - brassinosteroid KW - carotenoid KW - fruit KW - quality attributes SP - 105 EP - 15 JF - Plant biotechnology journal JO - Plant Biotechnol J VL - 12 IS - 1 N2 - The brassinosteroid (BR) response transcription factor Brassinazole resistant 1 (BZR1)-mediated BR signalling regulates many specific developmental processes including fruit ripening. Here, we report the effect of 2,4-epibrassinolide (EBR) and BZR1-1D overexpression on carotenoid accumulation and quality attributes of tomato (Solanum lycopersicum) fruit. EBR-treated pericarp discs of ethylene-insensitive mutant, Never ripe, accumulated significantly more carotenoid than those of the control. The results suggest that BR seems to be involved in modulating pigments accumulation. When three independent transgenic lines overexpressing the Arabidopsis BZR1-1D were used to evaluate the role of BZR1 in regulating tomato fruit carotenoid accumulation and quality attributes, fruits of all three transgenic lines exhibited enhanced carotenoid accumulation and increased soluble solid, soluble sugar and ascorbic acid contents during fruit ripening. In addition, the fruits of two transgenic lines showed dark green shoulder at mature green stage, in accordance with the up-regulated expression level of SlGLK2, which is involved in chloroplast development. Our results indicate the importance of BZR1-centred BR signalling in regulating carotenoid accumulation and quality attributes of tomato fruit and the potential application of the BZR1-like(s) for improvement of nutritional quality and flavour of tomato through genetic engineering. SN - 1467-7652 UR - https://www.unboundmedicine.com/medline/citation/24102834/Ectopic_expression_of_a_BZR1_1D_transcription_factor_in_brassinosteroid_signalling_enhances_carotenoid_accumulation_and_fruit_quality_attributes_in_tomato_ L2 - https://doi.org/10.1111/pbi.12121 DB - PRIME DP - Unbound Medicine ER -