Tags

Type your tag names separated by a space and hit enter

UHPLC determination of catechins for the quality control of green tea.
J Pharm Biomed Anal. 2014 Jan; 88:307-14.JP

Abstract

An ultra-high performance liquid chromatography (UHPLC) with UV detection method was developed for the fast quantitation of the most represented and biologically important green tea catechins and caffeine. UHPLC system was equipped with C18 analytical column (50mm×2.1mm, 1.8μm), utilizing a mobile phase composed of pH 2.5 triethanolamine phosphate buffer (0.1M) and acetonitrile in a gradient elution mode; under these conditions six major catechins and caffeine were separated in a 3min run. The method was fully validated in terms of precision, detection and quantification limits, linearity, accuracy, and it was applied to the identification and quantification of catechins and caffeine present in green tea infusions. In particular, commercially available green tea leaves samples of different geographical origin (Sencha, Ceylon Green and Lung Ching) were used for infusion preparations (water at 85°C for 15min). The selectivity of the developed UHPLC method was confirmed by comparison with UHPLC-MS/MS analysis. The recovery of the main six catechins and caffeine on the three analyzed commercial tea samples ranged from 94 to 108% (n=3). Limits of detection (LOD) were comprised in the range 0.1-0.4μgmL(-1). An orthogonal micellar electrokinetic (MEKC) method was applied for comparative purposes on selectivity and quantitative data. The combined use of the results obtained by the two techniques allowed for a fast confirmation on quantitative characterization of commercial samples.

Authors+Show Affiliations

Department of Pharmacy and Biotechnology, University of Bologna, via Belmeloro 6, 40126 Bologna, Italy.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

24103292

Citation

Naldi, Marina, et al. "UHPLC Determination of Catechins for the Quality Control of Green Tea." Journal of Pharmaceutical and Biomedical Analysis, vol. 88, 2014, pp. 307-14.
Naldi M, Fiori J, Gotti R, et al. UHPLC determination of catechins for the quality control of green tea. J Pharm Biomed Anal. 2014;88:307-14.
Naldi, M., Fiori, J., Gotti, R., Périat, A., Veuthey, J. L., Guillarme, D., & Andrisano, V. (2014). UHPLC determination of catechins for the quality control of green tea. Journal of Pharmaceutical and Biomedical Analysis, 88, 307-14. https://doi.org/10.1016/j.jpba.2013.08.054
Naldi M, et al. UHPLC Determination of Catechins for the Quality Control of Green Tea. J Pharm Biomed Anal. 2014;88:307-14. PubMed PMID: 24103292.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - UHPLC determination of catechins for the quality control of green tea. AU - Naldi,Marina, AU - Fiori,Jessica, AU - Gotti,Roberto, AU - Périat,Aurélie, AU - Veuthey,Jean-Luc, AU - Guillarme,Davy, AU - Andrisano,Vincenza, Y1 - 2013/09/12/ PY - 2013/08/19/received PY - 2013/08/28/accepted PY - 2013/10/10/entrez PY - 2013/10/10/pubmed PY - 2014/7/30/medline KW - (+)-catechin KW - (−)-catechin gallate KW - (−)-epicatechin KW - (−)-epicatechin gallate KW - (−)-epigallocatechin KW - (−)-epigallocatechin-gallate KW - (−)-gallocatechin KW - (−)-gallocatechin-gallate KW - C KW - CF KW - CG KW - Catechins KW - EC KW - ECG KW - EGC KW - EGCG KW - GC KW - GCG KW - Green tea KW - MEKC KW - TB KW - TF KW - UHPLC–MS KW - UHPLC–UV KW - caffeine KW - theobromine KW - theophylline SP - 307 EP - 14 JF - Journal of pharmaceutical and biomedical analysis JO - J Pharm Biomed Anal VL - 88 N2 - An ultra-high performance liquid chromatography (UHPLC) with UV detection method was developed for the fast quantitation of the most represented and biologically important green tea catechins and caffeine. UHPLC system was equipped with C18 analytical column (50mm×2.1mm, 1.8μm), utilizing a mobile phase composed of pH 2.5 triethanolamine phosphate buffer (0.1M) and acetonitrile in a gradient elution mode; under these conditions six major catechins and caffeine were separated in a 3min run. The method was fully validated in terms of precision, detection and quantification limits, linearity, accuracy, and it was applied to the identification and quantification of catechins and caffeine present in green tea infusions. In particular, commercially available green tea leaves samples of different geographical origin (Sencha, Ceylon Green and Lung Ching) were used for infusion preparations (water at 85°C for 15min). The selectivity of the developed UHPLC method was confirmed by comparison with UHPLC-MS/MS analysis. The recovery of the main six catechins and caffeine on the three analyzed commercial tea samples ranged from 94 to 108% (n=3). Limits of detection (LOD) were comprised in the range 0.1-0.4μgmL(-1). An orthogonal micellar electrokinetic (MEKC) method was applied for comparative purposes on selectivity and quantitative data. The combined use of the results obtained by the two techniques allowed for a fast confirmation on quantitative characterization of commercial samples. SN - 1873-264X UR - https://www.unboundmedicine.com/medline/citation/24103292/UHPLC_determination_of_catechins_for_the_quality_control_of_green_tea_ DB - PRIME DP - Unbound Medicine ER -