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Comparative analyses of mitochondrial and nuclear genetic markers for the molecular identification of Rhipicephalus spp.
Infect Genet Evol. 2013 Dec; 20:422-7.IG

Abstract

The genus Rhipicephalus (Acari: Ixodidae) comprises a large number of vectors of pathogens of substantial medical and veterinary concern; however, species identification based solely on morphological features is often challenging. In the present study, genetic distance within selected Rhipicephalus species (i.e., Rhipicephalus bursa, Rhipicephalus guilhoni, Rhipicephalus muhsamae, Rhipicephalus sanguineus sensu lato and Rhipicephalus turanicus), were investigated based on molecular and phylogenetic analyses of fragments of the mitochondrial 16S, 12S and cytochrome c oxidase subunit 1 (cox1) genes, as well as of the whole sequences of the ribosomal internal transcribed spacer-2 (ITS-2) region. Mean values of inter-specific genetic distance (e.g., up to 12.6%, 11.1% and 15.2%), as well as of intra-specific genetic distance (e.g., 0.9%, 0.9% and 1%), calculated using the Kimura-2 parameter substitution model with uniform rates among sites for 16S, 12S and cox1 genes, respectively, confirmed the differentiation of the rhipicephaline species herein examined. The molecular identification was also supported by the distinct separation of species-specific clades inferred from the phylogenetic analyses of all mitochondrial sequences. Conversely, little interspecific divergence was detected amongst ribosomal ITS-2 sequences (i.e., up to 2.8%) for species belonging to the R. sanguineus complex, which resulted in the ambiguous placement of selected R. sanguineus s.l. and R. turanicus sequences in the corresponding phylogenetic tree. Results from this study confirm the suitability of mtDNA markers for the reliable identification of ticks within the Rhipicephalus genus and provide a framework for future studies of taxonomy, speciation history and evolution of this group of ticks.

Authors+Show Affiliations

Department of Veterinary Medicine, University of Bari, 70010 Valenzano, Bari, Italy.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

24103336

Citation

Latrofa, Maria S., et al. "Comparative Analyses of Mitochondrial and Nuclear Genetic Markers for the Molecular Identification of Rhipicephalus Spp." Infection, Genetics and Evolution : Journal of Molecular Epidemiology and Evolutionary Genetics in Infectious Diseases, vol. 20, 2013, pp. 422-7.
Latrofa MS, Dantas-Torres F, Annoscia G, et al. Comparative analyses of mitochondrial and nuclear genetic markers for the molecular identification of Rhipicephalus spp. Infect Genet Evol. 2013;20:422-7.
Latrofa, M. S., Dantas-Torres, F., Annoscia, G., Cantacessi, C., & Otranto, D. (2013). Comparative analyses of mitochondrial and nuclear genetic markers for the molecular identification of Rhipicephalus spp. Infection, Genetics and Evolution : Journal of Molecular Epidemiology and Evolutionary Genetics in Infectious Diseases, 20, 422-7. https://doi.org/10.1016/j.meegid.2013.09.027
Latrofa MS, et al. Comparative Analyses of Mitochondrial and Nuclear Genetic Markers for the Molecular Identification of Rhipicephalus Spp. Infect Genet Evol. 2013;20:422-7. PubMed PMID: 24103336.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Comparative analyses of mitochondrial and nuclear genetic markers for the molecular identification of Rhipicephalus spp. AU - Latrofa,Maria S, AU - Dantas-Torres,Filipe, AU - Annoscia,Giada, AU - Cantacessi,Cinzia, AU - Otranto,Domenico, Y1 - 2013/10/06/ PY - 2013/07/30/received PY - 2013/09/24/revised PY - 2013/09/25/accepted PY - 2013/10/10/entrez PY - 2013/10/10/pubmed PY - 2014/8/19/medline KW - 12S KW - 16S KW - ITS-2 KW - Phylogeny KW - Rhipicephalus spp. KW - cox1 SP - 422 EP - 7 JF - Infection, genetics and evolution : journal of molecular epidemiology and evolutionary genetics in infectious diseases JO - Infect Genet Evol VL - 20 N2 - The genus Rhipicephalus (Acari: Ixodidae) comprises a large number of vectors of pathogens of substantial medical and veterinary concern; however, species identification based solely on morphological features is often challenging. In the present study, genetic distance within selected Rhipicephalus species (i.e., Rhipicephalus bursa, Rhipicephalus guilhoni, Rhipicephalus muhsamae, Rhipicephalus sanguineus sensu lato and Rhipicephalus turanicus), were investigated based on molecular and phylogenetic analyses of fragments of the mitochondrial 16S, 12S and cytochrome c oxidase subunit 1 (cox1) genes, as well as of the whole sequences of the ribosomal internal transcribed spacer-2 (ITS-2) region. Mean values of inter-specific genetic distance (e.g., up to 12.6%, 11.1% and 15.2%), as well as of intra-specific genetic distance (e.g., 0.9%, 0.9% and 1%), calculated using the Kimura-2 parameter substitution model with uniform rates among sites for 16S, 12S and cox1 genes, respectively, confirmed the differentiation of the rhipicephaline species herein examined. The molecular identification was also supported by the distinct separation of species-specific clades inferred from the phylogenetic analyses of all mitochondrial sequences. Conversely, little interspecific divergence was detected amongst ribosomal ITS-2 sequences (i.e., up to 2.8%) for species belonging to the R. sanguineus complex, which resulted in the ambiguous placement of selected R. sanguineus s.l. and R. turanicus sequences in the corresponding phylogenetic tree. Results from this study confirm the suitability of mtDNA markers for the reliable identification of ticks within the Rhipicephalus genus and provide a framework for future studies of taxonomy, speciation history and evolution of this group of ticks. SN - 1567-7257 UR - https://www.unboundmedicine.com/medline/citation/24103336/Comparative_analyses_of_mitochondrial_and_nuclear_genetic_markers_for_the_molecular_identification_of_Rhipicephalus_spp_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S1567-1348(13)00368-7 DB - PRIME DP - Unbound Medicine ER -