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Glycoprotein hormone alpha-subunit response to growth hormone (GH)-releasing hormone in patients with active acromegaly. Evidence for alpha-subunit and GH coexistence in the same tumoral cell.
J Clin Endocrinol Metab. 1985 Sep; 61(3):541-6.JC

Abstract

Basal serum concentrations of glycoprotein hormone alpha-subunit and its response to GH-releasing hormone (GHRH) were studied in 22 acromegalic patients and in normal subjects. Four out of 22 patients had a basal alpha-subunit concentration (1.2-3.5 ng/ml) clearly above the upper limit of the normal range. GHRH injection (1 microgram/kg body weight, bolus dose iv) produced a clear alpha-subunit response [mean % increase: 120 +/- 37 (SD)] in the 4 patients with elevated basal alpha-subunit levels. No increase in serum glycoprotein hormones (TSH, LH, and FSH) occurred. Selective adenomectomy in 2 patients resulted in normalization of both serum GH and alpha-subunit levels, as well as disappearance of the abnormal alpha-subunit response to GHRH. In in vitro studies, only these 2 adenomas secreted alpha-subunit in large amounts (534 and 388 ng/mg protein . 30 min) and was it further stimulated by GHRH (% increase: 83 and 126). Morphological studies done with protein A-gold particle immunotechnique demonstrated that in these adenomas the great majority of the cells contained secretory granules positive for both GH and alpha-subunit. We conclude that: 1) alpha-subunit hypersecretion is present in some acromegalic patients (about 20%), 2) GHRH stimulates alpha-subunit release both in vivo and in vitro only in patients with elevated basal alpha-subunit levels, and 3) in these patients alpha-subunit derives from a common adenomatous cell secreting both alpha-subunit and GH molecules.

Authors

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Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

2410443

Citation

Beck-Peccoz, P, et al. "Glycoprotein Hormone Alpha-subunit Response to Growth Hormone (GH)-releasing Hormone in Patients With Active Acromegaly. Evidence for Alpha-subunit and GH Coexistence in the Same Tumoral Cell." The Journal of Clinical Endocrinology and Metabolism, vol. 61, no. 3, 1985, pp. 541-6.
Beck-Peccoz P, Bassetti M, Spada A, et al. Glycoprotein hormone alpha-subunit response to growth hormone (GH)-releasing hormone in patients with active acromegaly. Evidence for alpha-subunit and GH coexistence in the same tumoral cell. J Clin Endocrinol Metab. 1985;61(3):541-6.
Beck-Peccoz, P., Bassetti, M., Spada, A., Medri, G., Arosio, M., Giannattasio, G., & Faglia, G. (1985). Glycoprotein hormone alpha-subunit response to growth hormone (GH)-releasing hormone in patients with active acromegaly. Evidence for alpha-subunit and GH coexistence in the same tumoral cell. The Journal of Clinical Endocrinology and Metabolism, 61(3), 541-6.
Beck-Peccoz P, et al. Glycoprotein Hormone Alpha-subunit Response to Growth Hormone (GH)-releasing Hormone in Patients With Active Acromegaly. Evidence for Alpha-subunit and GH Coexistence in the Same Tumoral Cell. J Clin Endocrinol Metab. 1985;61(3):541-6. PubMed PMID: 2410443.
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TY - JOUR T1 - Glycoprotein hormone alpha-subunit response to growth hormone (GH)-releasing hormone in patients with active acromegaly. Evidence for alpha-subunit and GH coexistence in the same tumoral cell. AU - Beck-Peccoz,P, AU - Bassetti,M, AU - Spada,A, AU - Medri,G, AU - Arosio,M, AU - Giannattasio,G, AU - Faglia,G, PY - 1985/9/1/pubmed PY - 1985/9/1/medline PY - 1985/9/1/entrez SP - 541 EP - 6 JF - The Journal of clinical endocrinology and metabolism JO - J Clin Endocrinol Metab VL - 61 IS - 3 N2 - Basal serum concentrations of glycoprotein hormone alpha-subunit and its response to GH-releasing hormone (GHRH) were studied in 22 acromegalic patients and in normal subjects. Four out of 22 patients had a basal alpha-subunit concentration (1.2-3.5 ng/ml) clearly above the upper limit of the normal range. GHRH injection (1 microgram/kg body weight, bolus dose iv) produced a clear alpha-subunit response [mean % increase: 120 +/- 37 (SD)] in the 4 patients with elevated basal alpha-subunit levels. No increase in serum glycoprotein hormones (TSH, LH, and FSH) occurred. Selective adenomectomy in 2 patients resulted in normalization of both serum GH and alpha-subunit levels, as well as disappearance of the abnormal alpha-subunit response to GHRH. In in vitro studies, only these 2 adenomas secreted alpha-subunit in large amounts (534 and 388 ng/mg protein . 30 min) and was it further stimulated by GHRH (% increase: 83 and 126). Morphological studies done with protein A-gold particle immunotechnique demonstrated that in these adenomas the great majority of the cells contained secretory granules positive for both GH and alpha-subunit. We conclude that: 1) alpha-subunit hypersecretion is present in some acromegalic patients (about 20%), 2) GHRH stimulates alpha-subunit release both in vivo and in vitro only in patients with elevated basal alpha-subunit levels, and 3) in these patients alpha-subunit derives from a common adenomatous cell secreting both alpha-subunit and GH molecules. SN - 0021-972X UR - https://www.unboundmedicine.com/medline/citation/2410443/Glycoprotein_hormone_alpha_subunit_response_to_growth_hormone__GH__releasing_hormone_in_patients_with_active_acromegaly__Evidence_for_alpha_subunit_and_GH_coexistence_in_the_same_tumoral_cell_ L2 - https://academic.oup.com/jcem/article-lookup/doi/10.1210/jcem-61-3-541 DB - PRIME DP - Unbound Medicine ER -