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Spatial distribution of nitrogen fixation in methane seep sediment and the role of the ANME archaea.
Environ Microbiol. 2014 Oct; 16(10):3012-29.EM

Abstract

Nitrogen (N2) fixation was investigated at Mound 12, Costa Rica, to determine its spatial distribution and biogeochemical controls in deep-sea methane seep sediment. Using (15)N2 tracer experiments and isotope ratio mass spectrometry analysis, we observed that seep N2 fixation is methane-dependent, and that N2 fixation rates peak in a narrow sediment depth horizon corresponding to increased abundance of aggregates of anaerobic methanotrophic archaea (ANME-2) and sulfate-reducing bacteria (SRB). Using fluorescence in situ hybridization coupled to nanoscale secondary ion mass spectrometry (FISH-NanoSIMS), we directly measured (15)N2 uptake by ANME-2/SRB aggregates (n = 26) and observed maximum (15)N incorporation within ANME-2-dominated areas of the aggregates, consistent with previous analyses. NanoSIMS analysis of single cells (n = 34) from the same microcosm experiment revealed no (15)N2 uptake. Together, these observations suggest that ANME-2, and possibly physically associated SRB, mediate the majority of new nitrogen production within the seep ecosystem. ANME-2 diazotrophy was observed while in association with members of two distinct orders of SRB: Desulfobacteraceae and Desulfobulbaceae. The rate of N2 fixation per unit volume biomass was independent of the identity of the associated SRB, aggregate size and morphology. Our results show that the distribution of seep N2 fixation is heterogeneous, laterally and with depth in the sediment, and is likely influenced by chemical gradients affecting the abundance and activity of ANME-2/SRB aggregates.

Authors+Show Affiliations

California Institute of Technology, Pasadena, CA, 91106, USA.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, Non-P.H.S.

Language

eng

PubMed ID

24107237

Citation

Dekas, Anne E., et al. "Spatial Distribution of Nitrogen Fixation in Methane Seep Sediment and the Role of the ANME Archaea." Environmental Microbiology, vol. 16, no. 10, 2014, pp. 3012-29.
Dekas AE, Chadwick GL, Bowles MW, et al. Spatial distribution of nitrogen fixation in methane seep sediment and the role of the ANME archaea. Environ Microbiol. 2014;16(10):3012-29.
Dekas, A. E., Chadwick, G. L., Bowles, M. W., Joye, S. B., & Orphan, V. J. (2014). Spatial distribution of nitrogen fixation in methane seep sediment and the role of the ANME archaea. Environmental Microbiology, 16(10), 3012-29. https://doi.org/10.1111/1462-2920.12247
Dekas AE, et al. Spatial Distribution of Nitrogen Fixation in Methane Seep Sediment and the Role of the ANME Archaea. Environ Microbiol. 2014;16(10):3012-29. PubMed PMID: 24107237.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Spatial distribution of nitrogen fixation in methane seep sediment and the role of the ANME archaea. AU - Dekas,Anne E, AU - Chadwick,Grayson L, AU - Bowles,Marshall W, AU - Joye,Samantha B, AU - Orphan,Victoria J, Y1 - 2013/09/24/ PY - 2013/02/11/received PY - 2013/05/24/revised PY - 2013/08/09/accepted PY - 2013/10/11/entrez PY - 2013/10/11/pubmed PY - 2015/1/30/medline SP - 3012 EP - 29 JF - Environmental microbiology JO - Environ. Microbiol. VL - 16 IS - 10 N2 - Nitrogen (N2) fixation was investigated at Mound 12, Costa Rica, to determine its spatial distribution and biogeochemical controls in deep-sea methane seep sediment. Using (15)N2 tracer experiments and isotope ratio mass spectrometry analysis, we observed that seep N2 fixation is methane-dependent, and that N2 fixation rates peak in a narrow sediment depth horizon corresponding to increased abundance of aggregates of anaerobic methanotrophic archaea (ANME-2) and sulfate-reducing bacteria (SRB). Using fluorescence in situ hybridization coupled to nanoscale secondary ion mass spectrometry (FISH-NanoSIMS), we directly measured (15)N2 uptake by ANME-2/SRB aggregates (n = 26) and observed maximum (15)N incorporation within ANME-2-dominated areas of the aggregates, consistent with previous analyses. NanoSIMS analysis of single cells (n = 34) from the same microcosm experiment revealed no (15)N2 uptake. Together, these observations suggest that ANME-2, and possibly physically associated SRB, mediate the majority of new nitrogen production within the seep ecosystem. ANME-2 diazotrophy was observed while in association with members of two distinct orders of SRB: Desulfobacteraceae and Desulfobulbaceae. The rate of N2 fixation per unit volume biomass was independent of the identity of the associated SRB, aggregate size and morphology. Our results show that the distribution of seep N2 fixation is heterogeneous, laterally and with depth in the sediment, and is likely influenced by chemical gradients affecting the abundance and activity of ANME-2/SRB aggregates. SN - 1462-2920 UR - https://www.unboundmedicine.com/medline/citation/24107237/Spatial_distribution_of_nitrogen_fixation_in_methane_seep_sediment_and_the_role_of_the_ANME_archaea_ L2 - https://doi.org/10.1111/1462-2920.12247 DB - PRIME DP - Unbound Medicine ER -