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Elevated concentrations of CCR7 ligands in patients with eosinophilic pneumonia.
Allergy. 2013 Nov; 68(11):1387-95.A

Abstract

BACKGROUND

Previous studies suggest that dendritic cells and macrophages play an important role in inflammation of eosinophilic pneumonia. The mechanism of dendritic cell and macrophage accumulation into the lung, however, is unknown. Here, we hypothesized that CCR7 ligands, CCL19 and CCL21, contribute to the accumulation of dendritic cells and alveolar macrophages in the inflamed lung of patients with eosinophilic pneumonia.

METHODS

Concentrations of the CCR7 ligands as well as CCL16, CCL17 and CCL22 in the bronchoalveolar lavage fluid of 53 patients with eosinophilic pneumonia, 29 patients with sarcoidosis, 18 patients with idiopathic pulmonary fibrosis and 12 healthy volunteers were measured by enzyme-linked immunosorbent assay. Cell sources of CCR7 ligands and CCR7-expressing cells in the bronchoalveolar lavage fluid were evaluated by immunocytochemistry.

RESULTS

CCL19 and CCL21 levels in the bronchoalveolar lavage fluid were significantly higher in patients with eosinophilic pneumonia than in controls. Levels of CCL19, but not CCL21, were statistically correlated with the levels of CCL16, CCL17 and CCL22 in patients with eosinophilic pneumonia. Immunocytochemistry revealed CCL19 expression in dendritic cells, macrophages and T-lymphocytes harvested from patients with eosinophilic pneumonia, and CCR7 expression in dendritic cells and macrophages. Levels of CCL19, but not CCL21, were significantly decreased after remission in patients with eosinophilic pneumonia. After provocation tests, CCL19 levels were elevated in all patients with eosinophilic pneumonia.

CONCLUSIONS

These findings indicate that CCL19 rather than CCL21 may contribute to the accumulation of dendritic cells and macrophages in the inflamed lungs of patients with eosinophilic pneumonia.

Authors+Show Affiliations

Department of Internal Medicine 3, Oita University Faculty of Medicine, Yufu, Japan.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

24111618

Citation

Nureki, S, et al. "Elevated Concentrations of CCR7 Ligands in Patients With Eosinophilic Pneumonia." Allergy, vol. 68, no. 11, 2013, pp. 1387-95.
Nureki S, Miyazaki E, Ishi T, et al. Elevated concentrations of CCR7 ligands in patients with eosinophilic pneumonia. Allergy. 2013;68(11):1387-95.
Nureki, S., Miyazaki, E., Ishi, T., Ito, T., Takenaka, R., Ando, M., & Kumamoto, T. (2013). Elevated concentrations of CCR7 ligands in patients with eosinophilic pneumonia. Allergy, 68(11), 1387-95. https://doi.org/10.1111/all.12243
Nureki S, et al. Elevated Concentrations of CCR7 Ligands in Patients With Eosinophilic Pneumonia. Allergy. 2013;68(11):1387-95. PubMed PMID: 24111618.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Elevated concentrations of CCR7 ligands in patients with eosinophilic pneumonia. AU - Nureki,S, AU - Miyazaki,E, AU - Ishi,T, AU - Ito,T, AU - Takenaka,R, AU - Ando,M, AU - Kumamoto,T, Y1 - 2013/09/21/ PY - 2013/07/20/accepted PY - 2013/10/12/entrez PY - 2013/10/12/pubmed PY - 2014/7/11/medline KW - CCL19 KW - CCL21 KW - CCR7 KW - bronchoalveolar lavage fluid KW - eosinophilic pneumonia SP - 1387 EP - 95 JF - Allergy JO - Allergy VL - 68 IS - 11 N2 - BACKGROUND: Previous studies suggest that dendritic cells and macrophages play an important role in inflammation of eosinophilic pneumonia. The mechanism of dendritic cell and macrophage accumulation into the lung, however, is unknown. Here, we hypothesized that CCR7 ligands, CCL19 and CCL21, contribute to the accumulation of dendritic cells and alveolar macrophages in the inflamed lung of patients with eosinophilic pneumonia. METHODS: Concentrations of the CCR7 ligands as well as CCL16, CCL17 and CCL22 in the bronchoalveolar lavage fluid of 53 patients with eosinophilic pneumonia, 29 patients with sarcoidosis, 18 patients with idiopathic pulmonary fibrosis and 12 healthy volunteers were measured by enzyme-linked immunosorbent assay. Cell sources of CCR7 ligands and CCR7-expressing cells in the bronchoalveolar lavage fluid were evaluated by immunocytochemistry. RESULTS: CCL19 and CCL21 levels in the bronchoalveolar lavage fluid were significantly higher in patients with eosinophilic pneumonia than in controls. Levels of CCL19, but not CCL21, were statistically correlated with the levels of CCL16, CCL17 and CCL22 in patients with eosinophilic pneumonia. Immunocytochemistry revealed CCL19 expression in dendritic cells, macrophages and T-lymphocytes harvested from patients with eosinophilic pneumonia, and CCR7 expression in dendritic cells and macrophages. Levels of CCL19, but not CCL21, were significantly decreased after remission in patients with eosinophilic pneumonia. After provocation tests, CCL19 levels were elevated in all patients with eosinophilic pneumonia. CONCLUSIONS: These findings indicate that CCL19 rather than CCL21 may contribute to the accumulation of dendritic cells and macrophages in the inflamed lungs of patients with eosinophilic pneumonia. SN - 1398-9995 UR - https://www.unboundmedicine.com/medline/citation/24111618/Elevated_concentrations_of_CCR7_ligands_in_patients_with_eosinophilic_pneumonia_ L2 - https://doi.org/10.1111/all.12243 DB - PRIME DP - Unbound Medicine ER -