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Fibre type-specific satellite cell content in two models of muscle disease.
Histopathology. 2013 Dec; 63(6):826-32.H

Abstract

AIMS

Muscle satellite cells (SCs) are responsible for the regenerative events following muscle fibre injury. This study aimed to improve our understanding of SC behaviour in two models of muscle disorder with different pathological mechanisms and onset of disease.

METHODS AND RESULTS

Pax7(+) SC content was assessed in types I and II fibres of patients with Duchenne muscular dystrophy (DMD; n = 9; age 13 ± 2 years), polymyositis/dermatomyositis (PM/DM; n = 9; age 52 ± 12 years) and in controls (n = 5; age 26 ± 5 years). Pax7(+) SCs number in type I and II fibres was higher (P < 0.05) in DMD and in PM/DM compared to controls. Type I fibres were associated with a higher number of Pax7(+) SCs compared to type II fibres only in DMD; Pax7(+) SCs number in type I fibres was about threefold higher in DMD compared to PM/DM (P < 0.05). In DMD, Pax7(+) SC content in small regenerating fibres (0.09 ± 0.09 SCs/fibre) was similar to that in fibres from healthy skeletal muscle. The proportion of activated SCs (Ki-67(+) SCs) was fivefold lower in DMD (0.4 ± 0.4%) compared to PM/DM (2.8 ± 2%). Pax7(+) cells located outside the basal lamina were observed in DMD muscles only.

CONCLUSION

The capacity to generate new SCs is increased even in severely impaired muscles and a fibre type-specific enhancement of SC occurs in type I muscle fibres in DMD.

Authors+Show Affiliations

Muscle and Exercise Physiology Research Group (MEP-RG), School of Health and Medical Sciences, Örebro University, Örebro, Sweden; Laboratory of Exercise Physiology (LPE EA 4338), University of Lyon, Saint-Étienne, France; Unit of Myology, University Hospital of Saint-Etienne, Saint-Étienne, France.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

24111647

Citation

Bankolé, Landry-Cyrille, et al. "Fibre Type-specific Satellite Cell Content in Two Models of Muscle Disease." Histopathology, vol. 63, no. 6, 2013, pp. 826-32.
Bankolé LC, Feasson L, Ponsot E, et al. Fibre type-specific satellite cell content in two models of muscle disease. Histopathology. 2013;63(6):826-32.
Bankolé, L. C., Feasson, L., Ponsot, E., & Kadi, F. (2013). Fibre type-specific satellite cell content in two models of muscle disease. Histopathology, 63(6), 826-32. https://doi.org/10.1111/his.12231
Bankolé LC, et al. Fibre Type-specific Satellite Cell Content in Two Models of Muscle Disease. Histopathology. 2013;63(6):826-32. PubMed PMID: 24111647.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Fibre type-specific satellite cell content in two models of muscle disease. AU - Bankolé,Landry-Cyrille, AU - Feasson,Léonard, AU - Ponsot,Elodie, AU - Kadi,Fawzi, Y1 - 2013/09/20/ PY - 2013/04/15/received PY - 2013/07/16/accepted PY - 2013/10/12/entrez PY - 2013/10/12/pubmed PY - 2014/8/15/medline KW - Duchenne muscular dystrophy KW - Pax7 KW - polymyositis and dermatomyositis KW - regeneration SP - 826 EP - 32 JF - Histopathology JO - Histopathology VL - 63 IS - 6 N2 - AIMS: Muscle satellite cells (SCs) are responsible for the regenerative events following muscle fibre injury. This study aimed to improve our understanding of SC behaviour in two models of muscle disorder with different pathological mechanisms and onset of disease. METHODS AND RESULTS: Pax7(+) SC content was assessed in types I and II fibres of patients with Duchenne muscular dystrophy (DMD; n = 9; age 13 ± 2 years), polymyositis/dermatomyositis (PM/DM; n = 9; age 52 ± 12 years) and in controls (n = 5; age 26 ± 5 years). Pax7(+) SCs number in type I and II fibres was higher (P < 0.05) in DMD and in PM/DM compared to controls. Type I fibres were associated with a higher number of Pax7(+) SCs compared to type II fibres only in DMD; Pax7(+) SCs number in type I fibres was about threefold higher in DMD compared to PM/DM (P < 0.05). In DMD, Pax7(+) SC content in small regenerating fibres (0.09 ± 0.09 SCs/fibre) was similar to that in fibres from healthy skeletal muscle. The proportion of activated SCs (Ki-67(+) SCs) was fivefold lower in DMD (0.4 ± 0.4%) compared to PM/DM (2.8 ± 2%). Pax7(+) cells located outside the basal lamina were observed in DMD muscles only. CONCLUSION: The capacity to generate new SCs is increased even in severely impaired muscles and a fibre type-specific enhancement of SC occurs in type I muscle fibres in DMD. SN - 1365-2559 UR - https://www.unboundmedicine.com/medline/citation/24111647/Fibre_type_specific_satellite_cell_content_in_two_models_of_muscle_disease_ L2 - https://doi.org/10.1111/his.12231 DB - PRIME DP - Unbound Medicine ER -