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Cranberry-derived proanthocyanidins prevent formation of Candida albicans biofilms in artificial urine through biofilm- and adherence-specific mechanisms.
J Antimicrob Chemother. 2014 Feb; 69(2):428-36.JA

Abstract

OBJECTIVES

Candida albicans is a common cause of nosocomial urinary tract infections (UTIs) and is responsible for increased morbidity and healthcare costs. Moreover, the US Centers for Medicare & Medicaid Services no longer reimburse for hospital-acquired catheter-associated UTIs. Thus, development of specific approaches for the prevention of Candida urinary infections is needed. Cranberry juice-derived proanthocyanidins (PACs) have efficacy in the prevention of bacterial UTIs, partially due to anti-adherence properties, but there are limited data on their use for the prevention and/or treatment of Candida UTIs. Therefore, we sought to systematically assess the in vitro effect of cranberry-derived PACs on C. albicans biofilm formation in artificial urine.

METHODS

C. albicans biofilms in artificial urine were coincubated with cranberry PACs at serially increasing concentrations and biofilm metabolic activity was assessed using the XTT assay in static microplate and silicone disc models.

RESULTS

Cranberry PAC concentrations of ≥16 mg/L significantly reduced biofilm formation in all C. albicans strains tested, with a paradoxical effect observed at high concentrations in two clinical isolates. Further, cranberry PACs were additive in combination with traditional antifungals. Cranberry PACs reduced C. albicans adherence to both polystyrene and silicone. Supplementation of the medium with iron reduced the efficacy of cranberry PACs against biofilms.

CONCLUSIONS

These findings indicate that cranberry PACs have excellent in vitro activity against C. albicans biofilm formation in artificial urine. We present preliminary evidence that cranberry PAC activity against C. albicans biofilm formation is due to anti-adherence properties and/or iron chelation.

Authors+Show Affiliations

Section of Infectious Diseases, New Mexico Veterans Healthcare System, Albuquerque, NM, USA.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, N.I.H., Extramural

Language

eng

PubMed ID

24114570

Citation

Rane, Hallie S., et al. "Cranberry-derived Proanthocyanidins Prevent Formation of Candida Albicans Biofilms in Artificial Urine Through Biofilm- and Adherence-specific Mechanisms." The Journal of Antimicrobial Chemotherapy, vol. 69, no. 2, 2014, pp. 428-36.
Rane HS, Bernardo SM, Howell AB, et al. Cranberry-derived proanthocyanidins prevent formation of Candida albicans biofilms in artificial urine through biofilm- and adherence-specific mechanisms. J Antimicrob Chemother. 2014;69(2):428-36.
Rane, H. S., Bernardo, S. M., Howell, A. B., & Lee, S. A. (2014). Cranberry-derived proanthocyanidins prevent formation of Candida albicans biofilms in artificial urine through biofilm- and adherence-specific mechanisms. The Journal of Antimicrobial Chemotherapy, 69(2), 428-36. https://doi.org/10.1093/jac/dkt398
Rane HS, et al. Cranberry-derived Proanthocyanidins Prevent Formation of Candida Albicans Biofilms in Artificial Urine Through Biofilm- and Adherence-specific Mechanisms. J Antimicrob Chemother. 2014;69(2):428-36. PubMed PMID: 24114570.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Cranberry-derived proanthocyanidins prevent formation of Candida albicans biofilms in artificial urine through biofilm- and adherence-specific mechanisms. AU - Rane,Hallie S, AU - Bernardo,Stella M, AU - Howell,Amy B, AU - Lee,Samuel A, Y1 - 2013/10/10/ PY - 2013/10/12/entrez PY - 2013/10/12/pubmed PY - 2014/9/10/medline KW - Vaccinium macrocarpon Ait. KW - cranberry PACs KW - urinary catheters KW - urinary tract infections SP - 428 EP - 36 JF - The Journal of antimicrobial chemotherapy JO - J. Antimicrob. Chemother. VL - 69 IS - 2 N2 - OBJECTIVES: Candida albicans is a common cause of nosocomial urinary tract infections (UTIs) and is responsible for increased morbidity and healthcare costs. Moreover, the US Centers for Medicare & Medicaid Services no longer reimburse for hospital-acquired catheter-associated UTIs. Thus, development of specific approaches for the prevention of Candida urinary infections is needed. Cranberry juice-derived proanthocyanidins (PACs) have efficacy in the prevention of bacterial UTIs, partially due to anti-adherence properties, but there are limited data on their use for the prevention and/or treatment of Candida UTIs. Therefore, we sought to systematically assess the in vitro effect of cranberry-derived PACs on C. albicans biofilm formation in artificial urine. METHODS: C. albicans biofilms in artificial urine were coincubated with cranberry PACs at serially increasing concentrations and biofilm metabolic activity was assessed using the XTT assay in static microplate and silicone disc models. RESULTS: Cranberry PAC concentrations of ≥16 mg/L significantly reduced biofilm formation in all C. albicans strains tested, with a paradoxical effect observed at high concentrations in two clinical isolates. Further, cranberry PACs were additive in combination with traditional antifungals. Cranberry PACs reduced C. albicans adherence to both polystyrene and silicone. Supplementation of the medium with iron reduced the efficacy of cranberry PACs against biofilms. CONCLUSIONS: These findings indicate that cranberry PACs have excellent in vitro activity against C. albicans biofilm formation in artificial urine. We present preliminary evidence that cranberry PAC activity against C. albicans biofilm formation is due to anti-adherence properties and/or iron chelation. SN - 1460-2091 UR - https://www.unboundmedicine.com/medline/citation/24114570/Cranberry_derived_proanthocyanidins_prevent_formation_of_Candida_albicans_biofilms_in_artificial_urine_through_biofilm__and_adherence_specific_mechanisms_ L2 - https://academic.oup.com/jac/article-lookup/doi/10.1093/jac/dkt398 DB - PRIME DP - Unbound Medicine ER -