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Determination of doxorubicin in plasma by using CE coupled with in-column tapered optic-fiber light-emitting diode induced fluorescence detection.
Electrophoresis. 2014 Mar; 35(5):762-9.E

Abstract

This paper proposes a novel strategy to enhance detection of doxorubicin in human plasma, using homemade CE combined with normal stacking mode (NSM). The detection system of CE named as in-column tapered optic-fiber light-emitting diode induced fluorescence detection system is economic and more sensitive that has been demonstrated in our previous work. The influence of sample matrix, BGE, applied voltage, and injection time on the efficiency of NSM were systematically investigated. The clean extracts were subjected to CE separation with optimal experimental conditions: Ethanol-water (1:1, v/v) was used as sample matrix, pH 4.12 15 mM sodium phosphate buffer solution containing 70% v/v ACN, applied voltage 23 kV and 45 s hydrodynamic injection at a height of 20 cm. The detection system displayed linear dynamic range from 6.4 to 1.13 × 10(3) ng/mL with a correlation coefficient of 0.9990 and LOD 2.2 ng/mL for doxorubicin (DOX). The proposed CE method has been successfully applied to determine DOX in human plasma which the recoveries of standard DOX added to human plasma were found to been the range of 93.8-104.6%. The results obtained demonstrate that our detection system combined with NSM is a good idea to enhance sensitivity in CE for routine determination of DOX in some biological specimens.

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Publisher Full Text (DOI)

Authors+Show Affiliations

Yang X
Chemical Synthesis and Pollution Control Key Laboratory of Sichuan Province, College of Chemistry and Chemical Engineering, China West Normal University, Nanchong, P. R. China.
Qian F
No affiliation info available
Xie L
No affiliation info available
Yang X
No affiliation info available
Cheng X
No affiliation info available
Choi MM
No affiliation info available

MeSH

BuffersDoxorubicinElectrophoresis, CapillaryEquipment DesignEthanolHumansOptical FibersReproducibility of ResultsSpectrometry, Fluorescence

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

24114803

Citation

Yang, Xiupei, et al. "Determination of Doxorubicin in Plasma By Using CE Coupled With In-column Tapered Optic-fiber Light-emitting Diode Induced Fluorescence Detection." Electrophoresis, vol. 35, no. 5, 2014, pp. 762-9.
Yang X, Qian F, Xie L, et al. Determination of doxorubicin in plasma by using CE coupled with in-column tapered optic-fiber light-emitting diode induced fluorescence detection. Electrophoresis. 2014;35(5):762-9.
Yang, X., Qian, F., Xie, L., Yang, X., Cheng, X., & Choi, M. M. (2014). Determination of doxorubicin in plasma by using CE coupled with in-column tapered optic-fiber light-emitting diode induced fluorescence detection. Electrophoresis, 35(5), 762-9. https://doi.org/10.1002/elps.201300237
Yang X, et al. Determination of Doxorubicin in Plasma By Using CE Coupled With In-column Tapered Optic-fiber Light-emitting Diode Induced Fluorescence Detection. Electrophoresis. 2014;35(5):762-9. PubMed PMID: 24114803.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Determination of doxorubicin in plasma by using CE coupled with in-column tapered optic-fiber light-emitting diode induced fluorescence detection. AU - Yang,Xiupei, AU - Qian,Fan, AU - Xie,Linxiang, AU - Yang,Xiaocui, AU - Cheng,Xiumei, AU - Choi,Martin M F, Y1 - 2013/11/04/ PY - 2013/05/19/received PY - 2013/09/04/revised PY - 2013/09/15/accepted PY - 2013/10/12/entrez PY - 2013/10/12/pubmed PY - 2015/5/13/medline KW - CE KW - Doxorubicin KW - LED KW - Normal stacking mode KW - Tapered optical-fiber SP - 762 EP - 9 JF - Electrophoresis JO - Electrophoresis VL - 35 IS - 5 N2 - This paper proposes a novel strategy to enhance detection of doxorubicin in human plasma, using homemade CE combined with normal stacking mode (NSM). The detection system of CE named as in-column tapered optic-fiber light-emitting diode induced fluorescence detection system is economic and more sensitive that has been demonstrated in our previous work. The influence of sample matrix, BGE, applied voltage, and injection time on the efficiency of NSM were systematically investigated. The clean extracts were subjected to CE separation with optimal experimental conditions: Ethanol-water (1:1, v/v) was used as sample matrix, pH 4.12 15 mM sodium phosphate buffer solution containing 70% v/v ACN, applied voltage 23 kV and 45 s hydrodynamic injection at a height of 20 cm. The detection system displayed linear dynamic range from 6.4 to 1.13 × 10(3) ng/mL with a correlation coefficient of 0.9990 and LOD 2.2 ng/mL for doxorubicin (DOX). The proposed CE method has been successfully applied to determine DOX in human plasma which the recoveries of standard DOX added to human plasma were found to been the range of 93.8-104.6%. The results obtained demonstrate that our detection system combined with NSM is a good idea to enhance sensitivity in CE for routine determination of DOX in some biological specimens. SN - 1522-2683 UR - https://www.unboundmedicine.com/medline/citation/24114803/Determination_of_doxorubicin_in_plasma_by_using_CE_coupled_with_in_column_tapered_optic_fiber_light_emitting_diode_induced_fluorescence_detection_ L2 - https://doi.org/10.1002/elps.201300237 DB - PRIME DP - Unbound Medicine ER -
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