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Biochemical and molecular characterisation and cross-resistance in field and laboratory chlorpyrifos-resistant strains of Laodelphax striatellus (Hemiptera: Delphacidae) from eastern China.
Pest Manag Sci. 2014 Jul; 70(7):1118-29.PM

Abstract

BACKGROUND

Laboratory selection is often employed in resistance mechanism studies because field-derived populations commonly do not have high enough resistance for such studies. In the present study, a field-collected Laodelphax striatellus population from eastern China was laboratory selected for chlorpyrifos resistance and susceptibility, and the developed strains, along with a field population, were studied for cross-resistance and resistance mechanisms at biochemical and molecular levels.

RESULTS

A 158.58-fold chlorpyrifos-resistant strain (JH-chl) and a chlorpyrifos-susceptible strain (JHS) were established after laboratory selection of 25 generations. Cross-resistance to deltamethrin, diazinon, methomyl, carbosulfan, acephate and imidacloprid were detected in JH-chl and a field-collected strain (JHF). Synergism and enzyme activity data suggested potential involvement of P450s and esterases in JH-chl as well as AChE alteration. Furthermore, CYP6AY3v2, CYP306A2v2, CYP353D1v2 and LSCE36 genes were significantly overexpressed in JH-chl (6.87-12.14-fold). Feeding of dsRNAs reduced the expression of the four target genes (35.6-56.8%) and caused significant adult mortality (75.21-88.45%), implying resistance reduction. However, mechanism(s) conferring chlorpyrifos resistance in JHF were unclear.

CONCLUSION

In contrast to previous reports, multiple overexpressed detoxification genes were potentially associated with chlorpyrifos resistance, as confirmed by RNAi feeding tests. Chlorpyrifos resistance exhibits cross-resistance with insecticides in the same and different classes.

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Publisher Full Text (DOI)

Authors+Show Affiliations

Xu L
Education Ministry Key Laboratory of Integrated Management of Crop Diseases and Pests, College of Plant Protection, Nanjing Agricultural University, Nanjing, China.
Wu M
No affiliation info available
Han Z
No affiliation info available

MeSH

Amino Acid SequenceAnimalsBase SequenceChinaChlorpyrifosCytochrome P-450 Enzyme SystemGene Expression RegulationHemipteraInsect ProteinsInsecticide ResistanceInsecticidesLethal Dose 50Molecular Sequence DataRNA InterferenceRNA, MessengerReal-Time Polymerase Chain ReactionSeasons

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

24115461

Citation

Xu, Lu, et al. "Biochemical and Molecular Characterisation and Cross-resistance in Field and Laboratory Chlorpyrifos-resistant Strains of Laodelphax Striatellus (Hemiptera: Delphacidae) From Eastern China." Pest Management Science, vol. 70, no. 7, 2014, pp. 1118-29.
Xu L, Wu M, Han Z. Biochemical and molecular characterisation and cross-resistance in field and laboratory chlorpyrifos-resistant strains of Laodelphax striatellus (Hemiptera: Delphacidae) from eastern China. Pest Manag Sci. 2014;70(7):1118-29.
Xu, L., Wu, M., & Han, Z. (2014). Biochemical and molecular characterisation and cross-resistance in field and laboratory chlorpyrifos-resistant strains of Laodelphax striatellus (Hemiptera: Delphacidae) from eastern China. Pest Management Science, 70(7), 1118-29. https://doi.org/10.1002/ps.3657
Xu L, Wu M, Han Z. Biochemical and Molecular Characterisation and Cross-resistance in Field and Laboratory Chlorpyrifos-resistant Strains of Laodelphax Striatellus (Hemiptera: Delphacidae) From Eastern China. Pest Manag Sci. 2014;70(7):1118-29. PubMed PMID: 24115461.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Biochemical and molecular characterisation and cross-resistance in field and laboratory chlorpyrifos-resistant strains of Laodelphax striatellus (Hemiptera: Delphacidae) from eastern China. AU - Xu,Lu, AU - Wu,Min, AU - Han,Zhaojun, Y1 - 2013/10/25/ PY - 2013/04/10/received PY - 2013/08/27/revised PY - 2013/09/20/accepted PY - 2013/10/12/entrez PY - 2013/10/12/pubmed PY - 2015/1/17/medline KW - P450 KW - RNAi KW - acetylcholinesterase KW - esterases KW - quantitative real-time RT-PCR KW - semi-quantitative RT-PCR KW - small brown planthopper SP - 1118 EP - 29 JF - Pest management science JO - Pest Manag Sci VL - 70 IS - 7 N2 - BACKGROUND: Laboratory selection is often employed in resistance mechanism studies because field-derived populations commonly do not have high enough resistance for such studies. In the present study, a field-collected Laodelphax striatellus population from eastern China was laboratory selected for chlorpyrifos resistance and susceptibility, and the developed strains, along with a field population, were studied for cross-resistance and resistance mechanisms at biochemical and molecular levels. RESULTS: A 158.58-fold chlorpyrifos-resistant strain (JH-chl) and a chlorpyrifos-susceptible strain (JHS) were established after laboratory selection of 25 generations. Cross-resistance to deltamethrin, diazinon, methomyl, carbosulfan, acephate and imidacloprid were detected in JH-chl and a field-collected strain (JHF). Synergism and enzyme activity data suggested potential involvement of P450s and esterases in JH-chl as well as AChE alteration. Furthermore, CYP6AY3v2, CYP306A2v2, CYP353D1v2 and LSCE36 genes were significantly overexpressed in JH-chl (6.87-12.14-fold). Feeding of dsRNAs reduced the expression of the four target genes (35.6-56.8%) and caused significant adult mortality (75.21-88.45%), implying resistance reduction. However, mechanism(s) conferring chlorpyrifos resistance in JHF were unclear. CONCLUSION: In contrast to previous reports, multiple overexpressed detoxification genes were potentially associated with chlorpyrifos resistance, as confirmed by RNAi feeding tests. Chlorpyrifos resistance exhibits cross-resistance with insecticides in the same and different classes. SN - 1526-4998 UR - https://www.unboundmedicine.com/medline/citation/24115461/Biochemical_and_molecular_characterisation_and_cross_resistance_in_field_and_laboratory_chlorpyrifos_resistant_strains_of_Laodelphax_striatellus__Hemiptera:_Delphacidae__from_eastern_China_ L2 - https://doi.org/10.1002/ps.3657 DB - PRIME DP - Unbound Medicine ER -