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Phospholipid:diacylglycerol acyltransferase-mediated triacylglycerol biosynthesis is crucial for protection against fatty acid-induced cell death in growing tissues of Arabidopsis.
Plant J. 2013 Dec; 76(6):930-42.PJ

Abstract

Phospholipid:diacylglycerol acyltransferase (PDAT) and diacylglycerol:acyl CoA acyltransferase play overlapping roles in triacylglycerol (TAG) assembly in Arabidopsis, and are essential for seed and pollen development, but the functional importance of PDAT in vegetative tissues remains largely unknown. Taking advantage of the Arabidopsis tgd1-1 mutant that accumulates oil in vegetative tissues, we demonstrate here that PDAT1 is crucial for TAG biosynthesis in growing tissues. We show that disruption of PDAT1 in the tgd1-1 mutant background causes serious growth retardation, gametophytic defects and premature cell death in developing leaves. Lipid analysis data indicated that knockout of PDAT1 results in increases in the levels of free fatty acids (FFAs) and diacylglycerol. In vivo ¹⁴C-acetate labeling experiments showed that, compared with wild-type, tgd1-1 exhibits a 3.8-fold higher rate of fatty acid synthesis (FAS), which is unaffected by disruption or over-expression of PDAT1, indicating a lack of feedback regulation of FAS in tgd1-1. We also show that detached leaves of both pdat1-2 and tgd1-1 pdat1-2 display increased sensitivity to FFA but not to diacylglycerol. Taken together, our results reveal a critical role for PDAT1 in mediating TAG synthesis and thereby protecting against FFA-induced cell death in fast-growing tissues of plants.

Authors+Show Affiliations

Biosciences Department, Brookhaven National Laboratory, Upton, NY, 11973, USA.No affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, U.S. Gov't, Non-P.H.S.

Language

eng

PubMed ID

24118513

Citation

Fan, Jilian, et al. "Phospholipid:diacylglycerol Acyltransferase-mediated Triacylglycerol Biosynthesis Is Crucial for Protection Against Fatty Acid-induced Cell Death in Growing Tissues of Arabidopsis." The Plant Journal : for Cell and Molecular Biology, vol. 76, no. 6, 2013, pp. 930-42.
Fan J, Yan C, Xu C. Phospholipid:diacylglycerol acyltransferase-mediated triacylglycerol biosynthesis is crucial for protection against fatty acid-induced cell death in growing tissues of Arabidopsis. Plant J. 2013;76(6):930-42.
Fan, J., Yan, C., & Xu, C. (2013). Phospholipid:diacylglycerol acyltransferase-mediated triacylglycerol biosynthesis is crucial for protection against fatty acid-induced cell death in growing tissues of Arabidopsis. The Plant Journal : for Cell and Molecular Biology, 76(6), 930-42. https://doi.org/10.1111/tpj.12343
Fan J, Yan C, Xu C. Phospholipid:diacylglycerol Acyltransferase-mediated Triacylglycerol Biosynthesis Is Crucial for Protection Against Fatty Acid-induced Cell Death in Growing Tissues of Arabidopsis. Plant J. 2013;76(6):930-42. PubMed PMID: 24118513.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Phospholipid:diacylglycerol acyltransferase-mediated triacylglycerol biosynthesis is crucial for protection against fatty acid-induced cell death in growing tissues of Arabidopsis. AU - Fan,Jilian, AU - Yan,Chengshi, AU - Xu,Changcheng, Y1 - 2013/11/05/ PY - 2013/07/25/received PY - 2013/09/25/revised PY - 2013/10/01/accepted PY - 2013/10/15/entrez PY - 2013/10/15/pubmed PY - 2014/9/24/medline KW - Arabidopsis thaliana KW - free fatty acid KW - phospholipid:diacylglycerol acyltransferase KW - premature cell death KW - triacylglycerol SP - 930 EP - 42 JF - The Plant journal : for cell and molecular biology JO - Plant J VL - 76 IS - 6 N2 - Phospholipid:diacylglycerol acyltransferase (PDAT) and diacylglycerol:acyl CoA acyltransferase play overlapping roles in triacylglycerol (TAG) assembly in Arabidopsis, and are essential for seed and pollen development, but the functional importance of PDAT in vegetative tissues remains largely unknown. Taking advantage of the Arabidopsis tgd1-1 mutant that accumulates oil in vegetative tissues, we demonstrate here that PDAT1 is crucial for TAG biosynthesis in growing tissues. We show that disruption of PDAT1 in the tgd1-1 mutant background causes serious growth retardation, gametophytic defects and premature cell death in developing leaves. Lipid analysis data indicated that knockout of PDAT1 results in increases in the levels of free fatty acids (FFAs) and diacylglycerol. In vivo ¹⁴C-acetate labeling experiments showed that, compared with wild-type, tgd1-1 exhibits a 3.8-fold higher rate of fatty acid synthesis (FAS), which is unaffected by disruption or over-expression of PDAT1, indicating a lack of feedback regulation of FAS in tgd1-1. We also show that detached leaves of both pdat1-2 and tgd1-1 pdat1-2 display increased sensitivity to FFA but not to diacylglycerol. Taken together, our results reveal a critical role for PDAT1 in mediating TAG synthesis and thereby protecting against FFA-induced cell death in fast-growing tissues of plants. SN - 1365-313X UR - https://www.unboundmedicine.com/medline/citation/24118513/Phospholipid:diacylglycerol_acyltransferase_mediated_triacylglycerol_biosynthesis_is_crucial_for_protection_against_fatty_acid_induced_cell_death_in_growing_tissues_of_Arabidopsis_ L2 - https://doi.org/10.1111/tpj.12343 DB - PRIME DP - Unbound Medicine ER -