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Transcriptional profile of Paracoccidioides induced by oenothein B, a potential antifungal agent from the Brazilian Cerrado plant Eugenia uniflora.
BMC Microbiol. 2013 Oct 12; 13:227.BM

Abstract

BACKGROUND

The compound oenothein B (OenB), which is isolated from the leaves of Eugenia uniflora, a Brazilian Cerrado plant, interferes with Paracoccidioides yeast cell morphology and inhibits 1,3-β-D-glucan synthase (PbFKS1) transcript accumulation, which is involved in cell wall synthesis. In this work we examined the gene expression changes in Paracoccidioides yeast cells following OenB treatment in order to investigate the adaptive cellular responses to drug stress.

RESULTS

We constructed differential gene expression libraries using Representational Difference Analysis (RDA) of Paracoccidioides yeast cells treated with OenB for 90 and 180 min. Treatment for 90 min resulted in the identification of 463 up-regulated expressed sequences tags (ESTs) and 104 down-regulated ESTs. For the 180 min treatment 301 up-regulated ESTs and 143 down-regulated were identified. Genes involved in the cell wall biosynthesis, such as GLN1, KRE6 and FKS1, were found to be regulated by OenB. Infection experiments in macrophages corroborated the in vitro results. Fluorescence microscopy showed increased levels of chitin in cells treated with OenB. The carbohydrate polymer content of the cell wall of the fungus was also evaluated, and the results corroborated with the transcriptional data. Several other genes, such as those involved in a variety of important cellular processes (i.e., membrane maintenance, stress and virulence) were found to be up-regulated in response to OenB treatment.

CONCLUSIONS

The exposure of Paracoccidioides to OenB resulted in a complex altered gene expression profile. Some of the changes may represent specific adaptive responses to this compound in this important pathogenic fungus.

Authors+Show Affiliations

Departamento de Bioquímica e Biologia Molecular, Laboratório de Biologia Molecular, Instituto de Ciências Biológicas, ICBII, Campus II, Universidade Federal de Goiás, C,P, 131, 74001-970 Goiânia, GO, Brazil. maristelaufg@gmail.com.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

24119145

Citation

Zambuzzi-Carvalho, Patrícia Fernanda, et al. "Transcriptional Profile of Paracoccidioides Induced By Oenothein B, a Potential Antifungal Agent From the Brazilian Cerrado Plant Eugenia Uniflora." BMC Microbiology, vol. 13, 2013, p. 227.
Zambuzzi-Carvalho PF, Tomazett PK, Santos SC, et al. Transcriptional profile of Paracoccidioides induced by oenothein B, a potential antifungal agent from the Brazilian Cerrado plant Eugenia uniflora. BMC Microbiol. 2013;13:227.
Zambuzzi-Carvalho, P. F., Tomazett, P. K., Santos, S. C., Ferri, P. H., Borges, C. L., Martins, W. S., de Almeida Soares, C. M., & Pereira, M. (2013). Transcriptional profile of Paracoccidioides induced by oenothein B, a potential antifungal agent from the Brazilian Cerrado plant Eugenia uniflora. BMC Microbiology, 13, 227. https://doi.org/10.1186/1471-2180-13-227
Zambuzzi-Carvalho PF, et al. Transcriptional Profile of Paracoccidioides Induced By Oenothein B, a Potential Antifungal Agent From the Brazilian Cerrado Plant Eugenia Uniflora. BMC Microbiol. 2013 Oct 12;13:227. PubMed PMID: 24119145.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Transcriptional profile of Paracoccidioides induced by oenothein B, a potential antifungal agent from the Brazilian Cerrado plant Eugenia uniflora. AU - Zambuzzi-Carvalho,Patrícia Fernanda, AU - Tomazett,Patrícia Kott, AU - Santos,Suzana Costa, AU - Ferri,Pedro Henrique, AU - Borges,Clayton Luiz, AU - Martins,Wellington Santos, AU - de Almeida Soares,Célia Maria, AU - Pereira,Maristela, Y1 - 2013/10/12/ PY - 2013/08/27/received PY - 2013/10/01/accepted PY - 2013/10/15/entrez PY - 2013/10/15/pubmed PY - 2014/7/1/medline SP - 227 EP - 227 JF - BMC microbiology JO - BMC Microbiol. VL - 13 N2 - BACKGROUND: The compound oenothein B (OenB), which is isolated from the leaves of Eugenia uniflora, a Brazilian Cerrado plant, interferes with Paracoccidioides yeast cell morphology and inhibits 1,3-β-D-glucan synthase (PbFKS1) transcript accumulation, which is involved in cell wall synthesis. In this work we examined the gene expression changes in Paracoccidioides yeast cells following OenB treatment in order to investigate the adaptive cellular responses to drug stress. RESULTS: We constructed differential gene expression libraries using Representational Difference Analysis (RDA) of Paracoccidioides yeast cells treated with OenB for 90 and 180 min. Treatment for 90 min resulted in the identification of 463 up-regulated expressed sequences tags (ESTs) and 104 down-regulated ESTs. For the 180 min treatment 301 up-regulated ESTs and 143 down-regulated were identified. Genes involved in the cell wall biosynthesis, such as GLN1, KRE6 and FKS1, were found to be regulated by OenB. Infection experiments in macrophages corroborated the in vitro results. Fluorescence microscopy showed increased levels of chitin in cells treated with OenB. The carbohydrate polymer content of the cell wall of the fungus was also evaluated, and the results corroborated with the transcriptional data. Several other genes, such as those involved in a variety of important cellular processes (i.e., membrane maintenance, stress and virulence) were found to be up-regulated in response to OenB treatment. CONCLUSIONS: The exposure of Paracoccidioides to OenB resulted in a complex altered gene expression profile. Some of the changes may represent specific adaptive responses to this compound in this important pathogenic fungus. SN - 1471-2180 UR - https://www.unboundmedicine.com/medline/citation/24119145/Transcriptional_profile_of_Paracoccidioides_induced_by_oenothein_B_a_potential_antifungal_agent_from_the_Brazilian_Cerrado_plant_Eugenia_uniflora_ L2 - https://bmcmicrobiol.biomedcentral.com/articles/10.1186/1471-2180-13-227 DB - PRIME DP - Unbound Medicine ER -