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A new ultrahigh performance liquid chromatography with diode array detection coupled to electrospray ionization and quadrupole time-of-flight mass spectrometry analytical strategy for fast analysis and improved characterization of phenolic compounds in apple products.
J Chromatogr A. 2013 Nov 05; 1316:78-91.JC

Abstract

A new, rapid, selective and sensitive ultrahigh performance liquid chromatography with diode array detection coupled to electrospray ionization and quadrupole time-of-flight mass spectrometry (UHPLC-DAD-ESI-Q-ToF-MS) strategy using automatic and simultaneous acquisition of exact mass at high and low collision energy, MS(E), has been developed to obtain polyphenolic profile of apples, apple pomace and apple juice from Asturian cider apples in a single run injection of 22 min. MS(E) spectral data acquisition overcomes chromatographic co-elution problems, performing simultaneous collection of precursor ions as well as other ions produced as a result of their fragmentation, which allows resolving complex spectra from mixtures of precursor ions in an unsupervised way and eases their interpretation. Using this technique, 52 phenolic compounds of five different classes were readily characterized in these apple extracts in both positive and negative ionization modes. The spectral data for phenolic compounds obtained using this acquisition mode are comparable to those obtained by conventional LC-MS/MS as exemplified in this work. Among the 52 phenolic compounds identified in this work, 2 dihydrochalcones and 3 flavonols have been tentatively identified for the first time in apple products. Moreover, 2 flavanols, 4 dihydrochalcones, 9 hydroxycinnamic acids and 4 flavonols had not been previously reported in apple by ToF analysis to our knowledge.

Authors+Show Affiliations

Departamento de Química Analítica, Facultad de Ciencia y Tecnología, Universidad del País Vasco/Euskal Herriko Unibertsitatea (UPV/EHU), P.O. Box 644, 48080 Bilbao, Spain.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

24120027

Citation

Ramirez-Ambrosi, M, et al. "A New Ultrahigh Performance Liquid Chromatography With Diode Array Detection Coupled to Electrospray Ionization and Quadrupole Time-of-flight Mass Spectrometry Analytical Strategy for Fast Analysis and Improved Characterization of Phenolic Compounds in Apple Products." Journal of Chromatography. A, vol. 1316, 2013, pp. 78-91.
Ramirez-Ambrosi M, Abad-Garcia B, Viloria-Bernal M, et al. A new ultrahigh performance liquid chromatography with diode array detection coupled to electrospray ionization and quadrupole time-of-flight mass spectrometry analytical strategy for fast analysis and improved characterization of phenolic compounds in apple products. J Chromatogr A. 2013;1316:78-91.
Ramirez-Ambrosi, M., Abad-Garcia, B., Viloria-Bernal, M., Garmon-Lobato, S., Berrueta, L. A., & Gallo, B. (2013). A new ultrahigh performance liquid chromatography with diode array detection coupled to electrospray ionization and quadrupole time-of-flight mass spectrometry analytical strategy for fast analysis and improved characterization of phenolic compounds in apple products. Journal of Chromatography. A, 1316, 78-91. https://doi.org/10.1016/j.chroma.2013.09.075
Ramirez-Ambrosi M, et al. A New Ultrahigh Performance Liquid Chromatography With Diode Array Detection Coupled to Electrospray Ionization and Quadrupole Time-of-flight Mass Spectrometry Analytical Strategy for Fast Analysis and Improved Characterization of Phenolic Compounds in Apple Products. J Chromatogr A. 2013 Nov 5;1316:78-91. PubMed PMID: 24120027.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - A new ultrahigh performance liquid chromatography with diode array detection coupled to electrospray ionization and quadrupole time-of-flight mass spectrometry analytical strategy for fast analysis and improved characterization of phenolic compounds in apple products. AU - Ramirez-Ambrosi,M, AU - Abad-Garcia,B, AU - Viloria-Bernal,M, AU - Garmon-Lobato,S, AU - Berrueta,L A, AU - Gallo,B, Y1 - 2013/09/29/ PY - 2013/06/18/received PY - 2013/08/30/revised PY - 2013/09/24/accepted PY - 2013/10/15/entrez PY - 2013/10/15/pubmed PY - 2014/5/20/medline KW - Apple products KW - Flavonoid KW - MS(E) KW - Mass spectrometry KW - Phenolic compound KW - UHPLC–Q-ToF SP - 78 EP - 91 JF - Journal of chromatography. A JO - J Chromatogr A VL - 1316 N2 - A new, rapid, selective and sensitive ultrahigh performance liquid chromatography with diode array detection coupled to electrospray ionization and quadrupole time-of-flight mass spectrometry (UHPLC-DAD-ESI-Q-ToF-MS) strategy using automatic and simultaneous acquisition of exact mass at high and low collision energy, MS(E), has been developed to obtain polyphenolic profile of apples, apple pomace and apple juice from Asturian cider apples in a single run injection of 22 min. MS(E) spectral data acquisition overcomes chromatographic co-elution problems, performing simultaneous collection of precursor ions as well as other ions produced as a result of their fragmentation, which allows resolving complex spectra from mixtures of precursor ions in an unsupervised way and eases their interpretation. Using this technique, 52 phenolic compounds of five different classes were readily characterized in these apple extracts in both positive and negative ionization modes. The spectral data for phenolic compounds obtained using this acquisition mode are comparable to those obtained by conventional LC-MS/MS as exemplified in this work. Among the 52 phenolic compounds identified in this work, 2 dihydrochalcones and 3 flavonols have been tentatively identified for the first time in apple products. Moreover, 2 flavanols, 4 dihydrochalcones, 9 hydroxycinnamic acids and 4 flavonols had not been previously reported in apple by ToF analysis to our knowledge. SN - 1873-3778 UR - https://www.unboundmedicine.com/medline/citation/24120027/A_new_ultrahigh_performance_liquid_chromatography_with_diode_array_detection_coupled_to_electrospray_ionization_and_quadrupole_time_of_flight_mass_spectrometry_analytical_strategy_for_fast_analysis_and_improved_characterization_of_phenolic_compounds_in_apple_products_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0021-9673(13)01542-2 DB - PRIME DP - Unbound Medicine ER -