Tags

Type your tag names separated by a space and hit enter

Thin layer chromatography-densitometric determination of some non-sedating antihistamines in combination with pseudoephedrine or acetaminophen in synthetic mixtures and in pharmaceutical formulations.
Biomed Chromatogr. 2014 Mar; 28(3):391-400.BC

Abstract

The combination of certain non-sedating antihistamines (NSA) such as fexofenadine (FXD), ketotifen (KET) and loratadine (LOR) with pseudoephedrine (PSE) or acetaminophen (ACE) is widely used in the treatment of allergic rhinitis, conjunctivitis and chronic urticaria. A rapid, simple, selective and precise densitometric method was developed and validated for simultaneous estimation of six synthetic binary mixtures and their pharmaceutical dosage forms. The method employed thin layer chromatography aluminum plates precoated with silica gel G 60 F254 as the stationary phase. The mobile phases chosen for development gave compact bands for the mixtures FXD-PSE (I), KET-PSE (II), LOR-PSE (III), FXD-ACE (IV), KET-ACE (V) and LOR-ACE (VI) [Retardation factor (Rf) values were (0.20, 0.32), (0.69, 0.34), (0.79, 0.13), (0.36, 0.70), (0.51, 0.30) and (0.76, 0.26), respectively]. Spectrodensitometric scanning integration was performed at 217, 218, 218, 233, 272 and 251 nm for the mixtures I-VI, respectively. The linear regression data for the calibration plots showed an excellent linear relationship. The method was validated for precision, accuracy, robustness and recovery. Limits of detection and quantitation were calculated. Statistical analysis proved that the method is reproducible and selective for the simultaneous estimation of these binary mixtures.

Links

Publisher Full Text (DOI)

Authors+Show Affiliations

El-Kommos ME
Department of Pharmaceutical Analytical Chemistry, Faculty of Pharmacy, Assiut University, Assiut, 71526, Egypt.
El-Gizawy SM
No affiliation info available
Atia NN
No affiliation info available
Hosny NM
No affiliation info available

MeSH

AcetaminophenChromatography, Thin LayerDensitometryHistamine H1 Antagonists, Non-SedatingLimit of DetectionLinear ModelsLoratadinePseudoephedrineReproducibility of ResultsTabletsTerfenadine

Pub Type(s)

Journal Article

Language

eng

PubMed ID

24123121

Citation

El-Kommos, Michael E., et al. "Thin Layer Chromatography-densitometric Determination of some Non-sedating Antihistamines in Combination With Pseudoephedrine or Acetaminophen in Synthetic Mixtures and in Pharmaceutical Formulations." Biomedical Chromatography : BMC, vol. 28, no. 3, 2014, pp. 391-400.
El-Kommos ME, El-Gizawy SM, Atia NN, et al. Thin layer chromatography-densitometric determination of some non-sedating antihistamines in combination with pseudoephedrine or acetaminophen in synthetic mixtures and in pharmaceutical formulations. Biomed Chromatogr. 2014;28(3):391-400.
El-Kommos, M. E., El-Gizawy, S. M., Atia, N. N., & Hosny, N. M. (2014). Thin layer chromatography-densitometric determination of some non-sedating antihistamines in combination with pseudoephedrine or acetaminophen in synthetic mixtures and in pharmaceutical formulations. Biomedical Chromatography : BMC, 28(3), 391-400. https://doi.org/10.1002/bmc.3033
El-Kommos ME, et al. Thin Layer Chromatography-densitometric Determination of some Non-sedating Antihistamines in Combination With Pseudoephedrine or Acetaminophen in Synthetic Mixtures and in Pharmaceutical Formulations. Biomed Chromatogr. 2014;28(3):391-400. PubMed PMID: 24123121.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Thin layer chromatography-densitometric determination of some non-sedating antihistamines in combination with pseudoephedrine or acetaminophen in synthetic mixtures and in pharmaceutical formulations. AU - El-Kommos,Michael E, AU - El-Gizawy,Samia M, AU - Atia,Noha N, AU - Hosny,Noha M, Y1 - 2013/09/30/ PY - 2013/06/19/received PY - 2013/08/12/revised PY - 2013/08/16/accepted PY - 2013/10/15/entrez PY - 2013/10/15/pubmed PY - 2014/9/24/medline KW - Non-sedating antihistamines KW - TLC-densitometry KW - acetaminophen KW - pharmaceutical analysis KW - pseudoephedrine SP - 391 EP - 400 JF - Biomedical chromatography : BMC JO - Biomed Chromatogr VL - 28 IS - 3 N2 - The combination of certain non-sedating antihistamines (NSA) such as fexofenadine (FXD), ketotifen (KET) and loratadine (LOR) with pseudoephedrine (PSE) or acetaminophen (ACE) is widely used in the treatment of allergic rhinitis, conjunctivitis and chronic urticaria. A rapid, simple, selective and precise densitometric method was developed and validated for simultaneous estimation of six synthetic binary mixtures and their pharmaceutical dosage forms. The method employed thin layer chromatography aluminum plates precoated with silica gel G 60 F254 as the stationary phase. The mobile phases chosen for development gave compact bands for the mixtures FXD-PSE (I), KET-PSE (II), LOR-PSE (III), FXD-ACE (IV), KET-ACE (V) and LOR-ACE (VI) [Retardation factor (Rf) values were (0.20, 0.32), (0.69, 0.34), (0.79, 0.13), (0.36, 0.70), (0.51, 0.30) and (0.76, 0.26), respectively]. Spectrodensitometric scanning integration was performed at 217, 218, 218, 233, 272 and 251 nm for the mixtures I-VI, respectively. The linear regression data for the calibration plots showed an excellent linear relationship. The method was validated for precision, accuracy, robustness and recovery. Limits of detection and quantitation were calculated. Statistical analysis proved that the method is reproducible and selective for the simultaneous estimation of these binary mixtures. SN - 1099-0801 UR - https://www.unboundmedicine.com/medline/citation/24123121/Thin_layer_chromatography_densitometric_determination_of_some_non_sedating_antihistamines_in_combination_with_pseudoephedrine_or_acetaminophen_in_synthetic_mixtures_and_in_pharmaceutical_formulations_ L2 - https://doi.org/10.1002/bmc.3033 DB - PRIME DP - Unbound Medicine ER -