Tags

Type your tag names separated by a space and hit enter

KPC-2-encoding plasmids from Escherichia coli and Klebsiella pneumoniae in Taiwan.
J Antimicrob Chemother. 2014 Mar; 69(3):628-31.JA

Abstract

OBJECTIVES

Two plasmids carrying bla(KPC-2) isolated from carbapenem-resistant Escherichia coli (CR-EC) and carbapenem-resistant Klebsiella pneumoniae (CR-KP), respectively, were completely sequenced. The CR-KP strain was selected from an outbreak in 2012, and the CR-EC strain was the first blaKPC-2-carrying E. coli identified in the same carbapenem resistance monitoring programme in Taiwan.

METHODS

Antimicrobial susceptibility tests, multilocus sequence typing (MLST) and the conjugal transfer of plasmids were performed. Complete sequencing of the plasmids was performed using a shotgun approach.

RESULTS

The CR-EC and CR-KP strains in this study were determined to be ST410 and ST11, respectively, by MLST. From CR-EC, we identified a 145 kb conjugative plasmid that carries bla(KPC-2), bla(CMY-2), bla(CTX-M-3) and bla(TEM-1). The plasmid is a chimera composed of three regions related to IncI, IncN and RepFIC replicons. From CR-KP, we identified an 86.5 kb plasmid, pKPC-LK30, which carries bla(KPC-2) and bla(SHV-11). The plasmid is very similar to two bla(KPC-2)-carrying IncFII(K) plasmids, but lacks one of the replication origins and cannot conjugate.

CONCLUSIONS

The differences in cross-species transferability of the two plasmids can be explained by genetic differences between their backbones and could have resulted in the confined bla(KPC-2)-carrying CR-KP outbreak in Taiwan. Plasmid pKPC-LKEc is the first bla(KPC-2)-carrying plasmid identified from CR-EC in Taiwan. With relatively high transferability it should be closely monitored.

Authors+Show Affiliations

Institute of Molecular and Genomic Medicine, National Health Research Institutes, Miaoli, Taiwan.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

24123430

Citation

Chen, Ying-Tsong, et al. "KPC-2-encoding Plasmids From Escherichia Coli and Klebsiella Pneumoniae in Taiwan." The Journal of Antimicrobial Chemotherapy, vol. 69, no. 3, 2014, pp. 628-31.
Chen YT, Lin JC, Fung CP, et al. KPC-2-encoding plasmids from Escherichia coli and Klebsiella pneumoniae in Taiwan. J Antimicrob Chemother. 2014;69(3):628-31.
Chen, Y. T., Lin, J. C., Fung, C. P., Lu, P. L., Chuang, Y. C., Wu, T. L., & Siu, L. K. (2014). KPC-2-encoding plasmids from Escherichia coli and Klebsiella pneumoniae in Taiwan. The Journal of Antimicrobial Chemotherapy, 69(3), 628-31. https://doi.org/10.1093/jac/dkt409
Chen YT, et al. KPC-2-encoding Plasmids From Escherichia Coli and Klebsiella Pneumoniae in Taiwan. J Antimicrob Chemother. 2014;69(3):628-31. PubMed PMID: 24123430.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - KPC-2-encoding plasmids from Escherichia coli and Klebsiella pneumoniae in Taiwan. AU - Chen,Ying-Tsong, AU - Lin,Jung-Chung, AU - Fung,Chang-Phone, AU - Lu,Po-Liang, AU - Chuang,Yin-Ching, AU - Wu,Tsu-Lan, AU - Siu,L Kristopher, Y1 - 2013/10/11/ PY - 2013/10/15/entrez PY - 2013/10/15/pubmed PY - 2015/7/16/medline KW - IncFIIK KW - carbapenemases KW - resistance mechanisms SP - 628 EP - 31 JF - The Journal of antimicrobial chemotherapy JO - J Antimicrob Chemother VL - 69 IS - 3 N2 - OBJECTIVES: Two plasmids carrying bla(KPC-2) isolated from carbapenem-resistant Escherichia coli (CR-EC) and carbapenem-resistant Klebsiella pneumoniae (CR-KP), respectively, were completely sequenced. The CR-KP strain was selected from an outbreak in 2012, and the CR-EC strain was the first blaKPC-2-carrying E. coli identified in the same carbapenem resistance monitoring programme in Taiwan. METHODS: Antimicrobial susceptibility tests, multilocus sequence typing (MLST) and the conjugal transfer of plasmids were performed. Complete sequencing of the plasmids was performed using a shotgun approach. RESULTS: The CR-EC and CR-KP strains in this study were determined to be ST410 and ST11, respectively, by MLST. From CR-EC, we identified a 145 kb conjugative plasmid that carries bla(KPC-2), bla(CMY-2), bla(CTX-M-3) and bla(TEM-1). The plasmid is a chimera composed of three regions related to IncI, IncN and RepFIC replicons. From CR-KP, we identified an 86.5 kb plasmid, pKPC-LK30, which carries bla(KPC-2) and bla(SHV-11). The plasmid is very similar to two bla(KPC-2)-carrying IncFII(K) plasmids, but lacks one of the replication origins and cannot conjugate. CONCLUSIONS: The differences in cross-species transferability of the two plasmids can be explained by genetic differences between their backbones and could have resulted in the confined bla(KPC-2)-carrying CR-KP outbreak in Taiwan. Plasmid pKPC-LKEc is the first bla(KPC-2)-carrying plasmid identified from CR-EC in Taiwan. With relatively high transferability it should be closely monitored. SN - 1460-2091 UR - https://www.unboundmedicine.com/medline/citation/24123430/KPC_2_encoding_plasmids_from_Escherichia_coli_and_Klebsiella_pneumoniae_in_Taiwan_ L2 - https://academic.oup.com/jac/article-lookup/doi/10.1093/jac/dkt409 DB - PRIME DP - Unbound Medicine ER -