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Rhinovirus-induced interferon production is not deficient in well controlled asthma.
Thorax 2014; 69(3):240-6T

Abstract

BACKGROUND

Defective rhinovirus (RV)-induced interferon (IFN)-β and IFN-λ production and increased RV replication have been reported in primary human bronchial epithelial cells (HBECs) from subjects with asthma. How universal this defect is in asthma is unknown. Additionally, the IFN subtypes induced by RV infection in primary HBECs have not been comprehensively investigated.

OBJECTIVE

To study RV induction of IFN-α, IFN-β and IFN-λ and RV replication in HBECs from subjects with atopic asthma and healthy controls.

METHODS

HBECs were obtained from subjects with asthma and healthy controls and infected with RV16 and RV1B, and cells and supernatants harvested at 8, 24 and 48h. IFN proteins were analysed by ELISA and IFN mRNA and viral RNA expression by quantitative PCR. Virus release was assessed in cell supernatants.

RESULTS

IFN-β and IFN-λ were the only IFNs induced by RV in HBECs and IFN-λ protein induction was substantially greater than IFN-β. Induction of IFN-λ1 mRNA by RV16 at 48h was significantly greater in HBECs from subjects with asthma; otherwise there were no significant differences between subjects with asthma and controls in RV replication, or in induction of type I or III IFN protein or mRNA.

CONCLUSIONS

IFN-λ and, to a lesser degree, IFN-β are the major IFN subtypes induced by RV infection of HBECs. Neither defective IFN induction by RV nor increased RV replication was observed in the HBECs from subjects with well controlled asthma reported in this study.

Authors+Show Affiliations

National Heart and Lung Institute, Imperial College London, , London, UK.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

24127021

Citation

Sykes, Annemarie, et al. "Rhinovirus-induced Interferon Production Is Not Deficient in Well Controlled Asthma." Thorax, vol. 69, no. 3, 2014, pp. 240-6.
Sykes A, Macintyre J, Edwards MR, et al. Rhinovirus-induced interferon production is not deficient in well controlled asthma. Thorax. 2014;69(3):240-6.
Sykes, A., Macintyre, J., Edwards, M. R., Del Rosario, A., Haas, J., Gielen, V., ... Johnston, S. L. (2014). Rhinovirus-induced interferon production is not deficient in well controlled asthma. Thorax, 69(3), pp. 240-6. doi:10.1136/thoraxjnl-2012-202909.
Sykes A, et al. Rhinovirus-induced Interferon Production Is Not Deficient in Well Controlled Asthma. Thorax. 2014;69(3):240-6. PubMed PMID: 24127021.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Rhinovirus-induced interferon production is not deficient in well controlled asthma. AU - Sykes,Annemarie, AU - Macintyre,Jonathan, AU - Edwards,Michael R, AU - Del Rosario,Ajerico, AU - Haas,Jennifer, AU - Gielen,Vera, AU - Kon,Onn Min, AU - McHale,Mark, AU - Johnston,Sebastian L, Y1 - 2013/10/14/ PY - 2013/10/16/entrez PY - 2013/10/16/pubmed PY - 2014/4/29/medline KW - Airway Epithelium KW - Asthma KW - Viral infection SP - 240 EP - 6 JF - Thorax JO - Thorax VL - 69 IS - 3 N2 - BACKGROUND: Defective rhinovirus (RV)-induced interferon (IFN)-β and IFN-λ production and increased RV replication have been reported in primary human bronchial epithelial cells (HBECs) from subjects with asthma. How universal this defect is in asthma is unknown. Additionally, the IFN subtypes induced by RV infection in primary HBECs have not been comprehensively investigated. OBJECTIVE: To study RV induction of IFN-α, IFN-β and IFN-λ and RV replication in HBECs from subjects with atopic asthma and healthy controls. METHODS: HBECs were obtained from subjects with asthma and healthy controls and infected with RV16 and RV1B, and cells and supernatants harvested at 8, 24 and 48h. IFN proteins were analysed by ELISA and IFN mRNA and viral RNA expression by quantitative PCR. Virus release was assessed in cell supernatants. RESULTS: IFN-β and IFN-λ were the only IFNs induced by RV in HBECs and IFN-λ protein induction was substantially greater than IFN-β. Induction of IFN-λ1 mRNA by RV16 at 48h was significantly greater in HBECs from subjects with asthma; otherwise there were no significant differences between subjects with asthma and controls in RV replication, or in induction of type I or III IFN protein or mRNA. CONCLUSIONS: IFN-λ and, to a lesser degree, IFN-β are the major IFN subtypes induced by RV infection of HBECs. Neither defective IFN induction by RV nor increased RV replication was observed in the HBECs from subjects with well controlled asthma reported in this study. SN - 1468-3296 UR - https://www.unboundmedicine.com/medline/citation/24127021/Rhinovirus_induced_interferon_production_is_not_deficient_in_well_controlled_asthma_ L2 - http://thorax.bmj.com/cgi/pmidlookup?view=long&pmid=24127021 DB - PRIME DP - Unbound Medicine ER -