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Molecular neuroanatomy and chemoarchitecture of the neurosecretory preoptic-hypothalamic area in zebrafish larvae.
J Comp Neurol. 2014 May 01; 522(7):1542-64.JC

Abstract

The paraventricular nucleus (PVN) in mammals is the main hypothalamic nucleus controlling hormone release in the pituitary and plays pivotal roles in homeostasis. While the location of a PVN-homologous region has been described in adult fish as the neurosecretory preoptic area (NPO), this region has not been clearly defined in larval zebrafish due to the difficulty in defining cytoarchitectonic nuclear boundaries in the larval brain. Here we identify the precise location of the larval zebrafish NPO using conserved transcription factor and neuropeptide gene expressions. Our results identify the dorsal half of the preoptic-hypothalamic orthopedia a (otpa) domain as the larval NPO and the homologous region to the mammalian PVN. Further, by reconstructing the locations of cells producing zebrafish neuropeptides found in the mammalian PVN (CCK, CRH, ENK, NTS, SS, VIP, OXT, AVP), we provide the first 3D arrangement map of NPO neuropeptides in the larval zebrafish brain. Our results show striking conservation of transcription factor expression (otp, arx, dlx5a, isl1) in and around the NPO/PVN together with neuropeptide expression within it. Finally, we describe the exact anatomical location of cells producing Oxt and Avp in the adult zebrafish. Thus, our results identify the definitive borders and extent of the PVN homologous region in larval zebrafish and serve as an important basis for cross-species comparisons of PVN/NPO structure and function.

Authors+Show Affiliations

Max Planck Institute for Medical Research, Developmental Genetics of the Nervous System, 69120, Heidelberg, Germany; The Hartmut Hoffmann-Berling International Graduate School of Molecular and Cellular Biology, University of Heidelberg, Germany.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

24127437

Citation

Herget, Ulrich, et al. "Molecular Neuroanatomy and Chemoarchitecture of the Neurosecretory Preoptic-hypothalamic Area in Zebrafish Larvae." The Journal of Comparative Neurology, vol. 522, no. 7, 2014, pp. 1542-64.
Herget U, Wolf A, Wullimann MF, et al. Molecular neuroanatomy and chemoarchitecture of the neurosecretory preoptic-hypothalamic area in zebrafish larvae. J Comp Neurol. 2014;522(7):1542-64.
Herget, U., Wolf, A., Wullimann, M. F., & Ryu, S. (2014). Molecular neuroanatomy and chemoarchitecture of the neurosecretory preoptic-hypothalamic area in zebrafish larvae. The Journal of Comparative Neurology, 522(7), 1542-64. https://doi.org/10.1002/cne.23480
Herget U, et al. Molecular Neuroanatomy and Chemoarchitecture of the Neurosecretory Preoptic-hypothalamic Area in Zebrafish Larvae. J Comp Neurol. 2014 May 1;522(7):1542-64. PubMed PMID: 24127437.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Molecular neuroanatomy and chemoarchitecture of the neurosecretory preoptic-hypothalamic area in zebrafish larvae. AU - Herget,Ulrich, AU - Wolf,Andrea, AU - Wullimann,Mario F, AU - Ryu,Soojin, PY - 2013/05/29/received PY - 2013/07/31/revised PY - 2013/09/30/accepted PY - 2013/10/16/entrez PY - 2013/10/16/pubmed PY - 2014/12/15/medline KW - hypothalamus KW - neuroendocrine system KW - neurosecretory preoptic area KW - paraventricular nucleus KW - preoptic region KW - zebrafish SP - 1542 EP - 64 JF - The Journal of comparative neurology JO - J. Comp. Neurol. VL - 522 IS - 7 N2 - The paraventricular nucleus (PVN) in mammals is the main hypothalamic nucleus controlling hormone release in the pituitary and plays pivotal roles in homeostasis. While the location of a PVN-homologous region has been described in adult fish as the neurosecretory preoptic area (NPO), this region has not been clearly defined in larval zebrafish due to the difficulty in defining cytoarchitectonic nuclear boundaries in the larval brain. Here we identify the precise location of the larval zebrafish NPO using conserved transcription factor and neuropeptide gene expressions. Our results identify the dorsal half of the preoptic-hypothalamic orthopedia a (otpa) domain as the larval NPO and the homologous region to the mammalian PVN. Further, by reconstructing the locations of cells producing zebrafish neuropeptides found in the mammalian PVN (CCK, CRH, ENK, NTS, SS, VIP, OXT, AVP), we provide the first 3D arrangement map of NPO neuropeptides in the larval zebrafish brain. Our results show striking conservation of transcription factor expression (otp, arx, dlx5a, isl1) in and around the NPO/PVN together with neuropeptide expression within it. Finally, we describe the exact anatomical location of cells producing Oxt and Avp in the adult zebrafish. Thus, our results identify the definitive borders and extent of the PVN homologous region in larval zebrafish and serve as an important basis for cross-species comparisons of PVN/NPO structure and function. SN - 1096-9861 UR - https://www.unboundmedicine.com/medline/citation/24127437/Molecular_neuroanatomy_and_chemoarchitecture_of_the_neurosecretory_preoptic_hypothalamic_area_in_zebrafish_larvae_ L2 - https://doi.org/10.1002/cne.23480 DB - PRIME DP - Unbound Medicine ER -