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Spica Prunellae extract inhibits the proliferation of human colon carcinoma cells via the regulation of the cell cycle.
Oncol Lett 2013; 6(4):1123-1127OL

Abstract

Spica Prunellae has long been used as a significant component in numerous traditional Chinese medicine (TCM) formulas to clinically treat cancers. Previously, Spica Prunellae was shown to promote cancer cell apoptosis and inhibit angiogenesis in vivo and in vitro. To further elucidate the precise mechanism of its tumoricidal activity, the effect of the ethanol extract of Spica Prunellae (EESP) on the proliferation of human colon carcinoma HT-29 cells was elucidated and the underlying molecular mechanisms were investigated. The proliferation of HT-29 cells was evaluated using 3-(4, 5-dimethyl-thiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) and colony formation analyses. The cell cycle was determined using fluorescence-activated cell sorting (FACS) with propidium iodide (PI) staining. The mRNA and protein expression of cyclin-dependent kinase 4 (CDK4) and cyclin D1 was examined using RT-PCR and western blotting, respectively. EESP was observed to inhibit HT-29 viability and survival in a dose- and time-dependent manner. Furthermore, EESP treatment blocked G1/S cell cycle progression and reduced the expression of pro-proliferative cyclin D1 and CDK4 at the transcriptional and translational levels. Altogether, these data suggest that the inhibition of cell proliferation via G1/S cell cycle arrest may be one of the mechanisms through which Spica Prunellae treats cancer.

Authors+Show Affiliations

Academy of Integrative Medicine, Fujian University of Traditional Chinese Medicine, Fuzhou, Fujian 350122, P.R. China ; Fujian Key Laboratory of Integrative Medicine on Geriatrics, Fujian University of Traditional Chinese Medicine, Fuzhou, Fujian 350122, P.R. China.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

24137475

Citation

Lin, Wei, et al. "Spica Prunellae Extract Inhibits the Proliferation of Human Colon Carcinoma Cells Via the Regulation of the Cell Cycle." Oncology Letters, vol. 6, no. 4, 2013, pp. 1123-1127.
Lin W, Zheng L, Zhuang Q, et al. Spica Prunellae extract inhibits the proliferation of human colon carcinoma cells via the regulation of the cell cycle. Oncol Lett. 2013;6(4):1123-1127.
Lin, W., Zheng, L., Zhuang, Q., Shen, A., Liu, L., Chen, Y., ... Peng, J. (2013). Spica Prunellae extract inhibits the proliferation of human colon carcinoma cells via the regulation of the cell cycle. Oncology Letters, 6(4), pp. 1123-1127.
Lin W, et al. Spica Prunellae Extract Inhibits the Proliferation of Human Colon Carcinoma Cells Via the Regulation of the Cell Cycle. Oncol Lett. 2013;6(4):1123-1127. PubMed PMID: 24137475.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Spica Prunellae extract inhibits the proliferation of human colon carcinoma cells via the regulation of the cell cycle. AU - Lin,Wei, AU - Zheng,Liangpu, AU - Zhuang,Qunchuan, AU - Shen,Aling, AU - Liu,Liya, AU - Chen,Youqin, AU - Sferra,Thomas J, AU - Peng,Jun, Y1 - 2013/08/05/ PY - 2013/05/07/received PY - 2013/07/24/accepted PY - 2013/10/19/entrez PY - 2013/10/19/pubmed PY - 2013/10/19/medline KW - Spica Prunellae KW - cell cycle KW - colorectal cancer KW - herbal medicine KW - proliferation SP - 1123 EP - 1127 JF - Oncology letters JO - Oncol Lett VL - 6 IS - 4 N2 - Spica Prunellae has long been used as a significant component in numerous traditional Chinese medicine (TCM) formulas to clinically treat cancers. Previously, Spica Prunellae was shown to promote cancer cell apoptosis and inhibit angiogenesis in vivo and in vitro. To further elucidate the precise mechanism of its tumoricidal activity, the effect of the ethanol extract of Spica Prunellae (EESP) on the proliferation of human colon carcinoma HT-29 cells was elucidated and the underlying molecular mechanisms were investigated. The proliferation of HT-29 cells was evaluated using 3-(4, 5-dimethyl-thiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) and colony formation analyses. The cell cycle was determined using fluorescence-activated cell sorting (FACS) with propidium iodide (PI) staining. The mRNA and protein expression of cyclin-dependent kinase 4 (CDK4) and cyclin D1 was examined using RT-PCR and western blotting, respectively. EESP was observed to inhibit HT-29 viability and survival in a dose- and time-dependent manner. Furthermore, EESP treatment blocked G1/S cell cycle progression and reduced the expression of pro-proliferative cyclin D1 and CDK4 at the transcriptional and translational levels. Altogether, these data suggest that the inhibition of cell proliferation via G1/S cell cycle arrest may be one of the mechanisms through which Spica Prunellae treats cancer. SN - 1792-1074 UR - https://www.unboundmedicine.com/medline/citation/24137475/Spica_Prunellae_extract_inhibits_the_proliferation_of_human_colon_carcinoma_cells_via_the_regulation_of_the_cell_cycle_ L2 - https://www.ingentaconnect.com/openurl?genre=article&issn=1792-1074&volume=6&issue=4&spage=1123&aulast=Lin DB - PRIME DP - Unbound Medicine ER -