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Alternative conformations of the ColE1 replication primer modulate its interaction with RNA I.
Cell. 1985 Oct; 42(3):959-66.Cell

Abstract

Replication of the ColE1 plasmid is regulated by the interaction of its primer RNA with a small countertranscript (RNA I) that acts as a repressor of functional primer formation. The interaction is dependent on the specific conformations of the complementary RNA molecules. Early in its synthesis, primer adopts an "anti-RNA I" configuration. As transcription proceeds, it is preempted by formation of an alternative domain designated stem-loop IV. This conformational transition has a significant effect on the rate of association of RNA I with the primer in vitro. Nascent primer in the "anti-RNA I" conformation (135 nucleotides) interacts with RNA I 6-fold faster than primer in the stem-loop IV conformation (241 nucleotides), and 35-fold faster than a 567 nucleotide primer precursor. We propose that a conformation-dependent "window of susceptibility" of primer to RNA I exists during primer transcription, and that altered conformations play a role in modulating the rate of functional primer formation.

Authors

No affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, U.S. Gov't, P.H.S.

Language

eng

PubMed ID

2414014

Citation

Wong, E M., and B Polisky. "Alternative Conformations of the ColE1 Replication Primer Modulate Its Interaction With RNA I." Cell, vol. 42, no. 3, 1985, pp. 959-66.
Wong EM, Polisky B. Alternative conformations of the ColE1 replication primer modulate its interaction with RNA I. Cell. 1985;42(3):959-66.
Wong, E. M., & Polisky, B. (1985). Alternative conformations of the ColE1 replication primer modulate its interaction with RNA I. Cell, 42(3), 959-66.
Wong EM, Polisky B. Alternative Conformations of the ColE1 Replication Primer Modulate Its Interaction With RNA I. Cell. 1985;42(3):959-66. PubMed PMID: 2414014.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Alternative conformations of the ColE1 replication primer modulate its interaction with RNA I. AU - Wong,E M, AU - Polisky,B, PY - 1985/10/1/pubmed PY - 1985/10/1/medline PY - 1985/10/1/entrez SP - 959 EP - 66 JF - Cell JO - Cell VL - 42 IS - 3 N2 - Replication of the ColE1 plasmid is regulated by the interaction of its primer RNA with a small countertranscript (RNA I) that acts as a repressor of functional primer formation. The interaction is dependent on the specific conformations of the complementary RNA molecules. Early in its synthesis, primer adopts an "anti-RNA I" configuration. As transcription proceeds, it is preempted by formation of an alternative domain designated stem-loop IV. This conformational transition has a significant effect on the rate of association of RNA I with the primer in vitro. Nascent primer in the "anti-RNA I" conformation (135 nucleotides) interacts with RNA I 6-fold faster than primer in the stem-loop IV conformation (241 nucleotides), and 35-fold faster than a 567 nucleotide primer precursor. We propose that a conformation-dependent "window of susceptibility" of primer to RNA I exists during primer transcription, and that altered conformations play a role in modulating the rate of functional primer formation. SN - 0092-8674 UR - https://www.unboundmedicine.com/medline/citation/2414014/Alternative_conformations_of_the_ColE1_replication_primer_modulate_its_interaction_with_RNA_I_ L2 - https://linkinghub.elsevier.com/retrieve/pii/0092-8674(85)90292-2 DB - PRIME DP - Unbound Medicine ER -