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Modulation of liver function, antioxidant responses, insulin resistance and glucose transport by Oroxylum indicum stem bark in STZ induced diabetic rats.
Food Chem Toxicol. 2013 Dec; 62:722-31.FC

Abstract

A decoction of stem bark of Oroxylum indicum Vent. (OI) is taken (2-3 times/day) by the tribal people of Sikkim, India to treat diabetes but scientific validation of its overall potential is lacking. Present study was aimed to assess in vitro antihyperglycemic activity of standardized OI extract using inhibition of α-glucosidase, BSA glycation and enhancement of insulin sensitivity. Antidiabetic and antioxidant modulatory effects of OI extract along with the blood biomarkers of toxic response were studied in streptozotocin (STZ) induced diabetic rats. In vitro analysis showed strong antioxidant capacity of OI -and potential to inhibit BSA glycation and α-glucosidase activity which was comparable to standard counterparts. Extract also improved insulin sensitivity in mature 3T3-L1 adipocytes. In vivo effects of OI extract (oral 250 mg/kg b.wt.) on STZ induced type II diabetic rats normalized the antioxidant status (p≤0.01). Analysis of blood biomarkers of toxic response indicated its safety. Lowering of total cholesterol and HDL levels (p≤0.05) and restoration of glycated Hb (p≤0.01) were also found in OI treated diabetic rats. HOMA-IR, QUICKI analysis along with area under the curve analysis showed the capacity of OI extract to enhance the insulin sensitivity significantly (p≤0.01) which was confirmed by increased GLUT-4 translocation in skeletal muscles.

Authors+Show Affiliations

Herbal Research Section, CSIR-Indian Institute of Toxicology Research, Post Box No. 80, Mahatma Gandhi Marg, Lucknow 226001, UP, India.No affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

24140466

Citation

Singh, Jyotsna, and Poonam Kakkar. "Modulation of Liver Function, Antioxidant Responses, Insulin Resistance and Glucose Transport By Oroxylum Indicum Stem Bark in STZ Induced Diabetic Rats." Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association, vol. 62, 2013, pp. 722-31.
Singh J, Kakkar P. Modulation of liver function, antioxidant responses, insulin resistance and glucose transport by Oroxylum indicum stem bark in STZ induced diabetic rats. Food Chem Toxicol. 2013;62:722-31.
Singh, J., & Kakkar, P. (2013). Modulation of liver function, antioxidant responses, insulin resistance and glucose transport by Oroxylum indicum stem bark in STZ induced diabetic rats. Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association, 62, 722-31. https://doi.org/10.1016/j.fct.2013.09.035
Singh J, Kakkar P. Modulation of Liver Function, Antioxidant Responses, Insulin Resistance and Glucose Transport By Oroxylum Indicum Stem Bark in STZ Induced Diabetic Rats. Food Chem Toxicol. 2013;62:722-31. PubMed PMID: 24140466.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Modulation of liver function, antioxidant responses, insulin resistance and glucose transport by Oroxylum indicum stem bark in STZ induced diabetic rats. AU - Singh,Jyotsna, AU - Kakkar,Poonam, Y1 - 2013/10/15/ PY - 2013/07/01/received PY - 2013/09/26/revised PY - 2013/09/29/accepted PY - 2013/10/22/entrez PY - 2013/10/22/pubmed PY - 2014/8/16/medline KW - (4-NPGP) KW - (ABTS) KW - (ADA) KW - (AGE) KW - (ALT) KW - (ANOVA) KW - (AST) KW - (AUC) KW - (Akt/PKB) KW - (BSA) KW - (BUN) KW - (CPCSEA) KW - (DM) KW - (DMEM) KW - (DNPH) KW - (DTNB) KW - (FBG) KW - (FBS) KW - (FITC) KW - (FRAP) KW - (GLUT-4) KW - (GSH) KW - (HCT) KW - (HDL) KW - (HOMA-IR) KW - (HRP) KW - (Hb) KW - (HbA1c) KW - (IR) KW - (LDL) KW - (MCV) KW - (MDA) KW - (NA) KW - (OGTT) KW - (OI) KW - (PBS) KW - (PC) KW - (PI3K) KW - (PVDF) KW - (QUICKI) KW - (RBC) KW - (ROS) KW - (RP-HPLC) KW - (SDS–PAGE) KW - (STZ) KW - (TBA) KW - (TC) KW - (TEAC) KW - (TG) KW - (TPTZ) KW - (WBC) KW - (WHO) KW - 2,2′-azinobis (3-ethylbenzothiazoline-6-sulphonic acid KW - 2,4,6-tripyridyl-s-triazine KW - 2,4-dinitrophenyl hydrazine KW - 4-nitrophenyl p-d glucopyranoside KW - 5,5′dithiobis-2-nitrobenzoic acid KW - American diabetes association KW - Anti diabetic activity KW - Antioxidant KW - BSA glycation KW - Diabetes mellitus KW - Dulbecco’s Modified Eagle Medium KW - Insulin sensitivity KW - Oral Glucose Tolerance Test KW - Oroxylum indicum KW - Oroxylum indicum (OI) KW - Trolox equivalent antioxidant capacity KW - World Health Organization KW - advanced glycation end products KW - alanine amino transferase KW - analysis of variance KW - area under the curve KW - aspartate amino transferase KW - blood urea nitrogen KW - bovine serum albumin KW - committee for the purpose of control and supervision of experiments on animals KW - differentiation media KW - fasting blood glucose KW - ferric ion reducing antioxidant power KW - fetal bovine serum KW - fluorescein isothiocyanate KW - glucose transporter-4 KW - glycosylated hemoglobin KW - heamatocrit KW - hemoglobin KW - high density lipoprotein cholesterol KW - homeostatic model assessment of Insulin resistance KW - horse-radish peroxidase KW - insulin receptor KW - low density lipoprotein cholesterol KW - malondialdehyde KW - mean corpuscular volume KW - nicotinamide KW - phosphate buffer saline pH 7.4 KW - phosphatidylinositol-3-kinase KW - polyvinylidene difluoride KW - protein carbonyls KW - protein kinase B KW - quantitative insulin sensitivity check index KW - reactive oxygen species KW - red blood cell counts KW - reduced glutathione KW - reverse phase high performance liquid chromatography KW - sodium dodecyl sulphate–polyacrylamide gel electrophoresis KW - streptozotocin KW - thiobarbituric acid KW - total cholesterol KW - total glycerides KW - white blood cell counts SP - 722 EP - 31 JF - Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association JO - Food Chem Toxicol VL - 62 N2 - A decoction of stem bark of Oroxylum indicum Vent. (OI) is taken (2-3 times/day) by the tribal people of Sikkim, India to treat diabetes but scientific validation of its overall potential is lacking. Present study was aimed to assess in vitro antihyperglycemic activity of standardized OI extract using inhibition of α-glucosidase, BSA glycation and enhancement of insulin sensitivity. Antidiabetic and antioxidant modulatory effects of OI extract along with the blood biomarkers of toxic response were studied in streptozotocin (STZ) induced diabetic rats. In vitro analysis showed strong antioxidant capacity of OI -and potential to inhibit BSA glycation and α-glucosidase activity which was comparable to standard counterparts. Extract also improved insulin sensitivity in mature 3T3-L1 adipocytes. In vivo effects of OI extract (oral 250 mg/kg b.wt.) on STZ induced type II diabetic rats normalized the antioxidant status (p≤0.01). Analysis of blood biomarkers of toxic response indicated its safety. Lowering of total cholesterol and HDL levels (p≤0.05) and restoration of glycated Hb (p≤0.01) were also found in OI treated diabetic rats. HOMA-IR, QUICKI analysis along with area under the curve analysis showed the capacity of OI extract to enhance the insulin sensitivity significantly (p≤0.01) which was confirmed by increased GLUT-4 translocation in skeletal muscles. SN - 1873-6351 UR - https://www.unboundmedicine.com/medline/citation/24140466/Modulation_of_liver_function_antioxidant_responses_insulin_resistance_and_glucose_transport_by_Oroxylum_indicum_stem_bark_in_STZ_induced_diabetic_rats_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0278-6915(13)00673-X DB - PRIME DP - Unbound Medicine ER -