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Antioxidant and hepatoprotective activity of Fagonia schweinfurthii (Hadidi) Hadidi extract in carbon tetrachloride induced hepatotoxicity in HepG2 cell line and rats.
J Ethnopharmacol 2013; 150(3):973-81JE

Abstract

ETHNOPHARMACOLOGICAL RELEVANCE

The whole plant of Fagonia schweinfurthii (Hadidi) Hadidi (Family: Zygophyllaceae) is used in variety of diseases including hepatic ailments in deserts and dry areas of India.

AIM OF THE STUDY

To evaluate antioxidant and hepatoprotective activity of ethanolic extract from Fagonia schweinfurthii (Hadidi) Hadidi (FSEE) in carbon tetrachloride (CCl4) induced hepatotoxicity in HepG2 cell line and rats.

MATERIALS AND METHODS

In vitro antioxidant activity was determined by DPPH, ABTS radicals and hydrogen peroxide methods. In vitro cytotoxicity and hepatoprotective potential of FSEE were evaluated using HepG2 cells. Based on the cytotoxicity assay, FSEE (50, 100, 200 µg/ml) was assessed for hepatoprotective potential against CCl4 induced toxicity in HepG2 cell line by monitoring cell viability, aspartate aminotransferase (AST), alanine aminotransaminase (ALT), lactate dehydrogenase (LDH) leakage, lipid peroxidation (LPO) and glutathione level (GSH). Further, in vivo hepatoprotective activity of FSEE was evaluated against CCl4 induced hepatotoxicity in male Wistar albino rats. Rats were pre-treated with FSEE (200 mg, 400 mg kg(-1) day(-1) p.o.) for 7 days followed by a single dose of CCl4 (1.0 ml/kg, i.p.) on 8th day. Silymarin was used as positive control. After 24h of CCl4 administration, various biochemical parameters like aspartate aminotransferase (AST), alanine aminotransaminase (ALT), alkaline phosphatase (ALP), total bilirubin (TB) and total protein (TP) levels were estimated in serum. The antioxidant parameters like superoxide dismutase (SOD) activity, catalase (CAT) activity, glutathione (GSH) content and malondialdehyde (MDA) level in the liver homogenate were determined. Histopathological changes in the liver of different groups were also studied.

RESULTS

The FSEE possessed strong antioxidant activity in vitro. The results indicated that CCl4 treatment caused a significant decrease in cell viability. The CCl4-induced changes in the HepG2 cells were significantly ameliorated by treatment of the FSEE. FSEE significantly prevented CCl4 induced elevation of AST, ALT, ALP, TB, and CCl4 induced decrease in total protein in rats. FSEE treated rat liver anti-oxidant parameters (SOD, CAT, MDA and GSH,) were significantly antagonized for the pro-oxidant effect of CCl4. Histopathological studies also supported the protective effect of FSEE.

CONCLUSION

The results of this study revealed that FSEE has significant hepatoprotective activity. This effect may be due to the ability of the extract to inhibit lipid peroxidation and increase in the anti-oxidant enzymatic activity.

Authors+Show Affiliations

Lachoo Memorial College of Science & Technology (Autonomous), Pharmacy Wing, Jodhpur 342003, Rajasthan, India. Electronic address: pareekworld@gmail.com.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

24140589

Citation

Pareek, Anil, et al. "Antioxidant and Hepatoprotective Activity of Fagonia Schweinfurthii (Hadidi) Hadidi Extract in Carbon Tetrachloride Induced Hepatotoxicity in HepG2 Cell Line and Rats." Journal of Ethnopharmacology, vol. 150, no. 3, 2013, pp. 973-81.
Pareek A, Godavarthi A, Issarani R, et al. Antioxidant and hepatoprotective activity of Fagonia schweinfurthii (Hadidi) Hadidi extract in carbon tetrachloride induced hepatotoxicity in HepG2 cell line and rats. J Ethnopharmacol. 2013;150(3):973-81.
Pareek, A., Godavarthi, A., Issarani, R., & Nagori, B. P. (2013). Antioxidant and hepatoprotective activity of Fagonia schweinfurthii (Hadidi) Hadidi extract in carbon tetrachloride induced hepatotoxicity in HepG2 cell line and rats. Journal of Ethnopharmacology, 150(3), pp. 973-81. doi:10.1016/j.jep.2013.09.048.
Pareek A, et al. Antioxidant and Hepatoprotective Activity of Fagonia Schweinfurthii (Hadidi) Hadidi Extract in Carbon Tetrachloride Induced Hepatotoxicity in HepG2 Cell Line and Rats. J Ethnopharmacol. 2013 Dec 12;150(3):973-81. PubMed PMID: 24140589.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Antioxidant and hepatoprotective activity of Fagonia schweinfurthii (Hadidi) Hadidi extract in carbon tetrachloride induced hepatotoxicity in HepG2 cell line and rats. AU - Pareek,Anil, AU - Godavarthi,Ashok, AU - Issarani,Roshan, AU - Nagori,Badri Prakash, Y1 - 2013/10/17/ PY - 2013/03/02/received PY - 2013/09/08/revised PY - 2013/09/24/accepted PY - 2013/10/22/entrez PY - 2013/10/22/pubmed PY - 2014/8/26/medline KW - Antioxidant KW - CCl(4) KW - Fagonia schweinfurthii KW - HepG2 cell line KW - Hepatoprotective SP - 973 EP - 81 JF - Journal of ethnopharmacology JO - J Ethnopharmacol VL - 150 IS - 3 N2 - ETHNOPHARMACOLOGICAL RELEVANCE: The whole plant of Fagonia schweinfurthii (Hadidi) Hadidi (Family: Zygophyllaceae) is used in variety of diseases including hepatic ailments in deserts and dry areas of India. AIM OF THE STUDY: To evaluate antioxidant and hepatoprotective activity of ethanolic extract from Fagonia schweinfurthii (Hadidi) Hadidi (FSEE) in carbon tetrachloride (CCl4) induced hepatotoxicity in HepG2 cell line and rats. MATERIALS AND METHODS: In vitro antioxidant activity was determined by DPPH, ABTS radicals and hydrogen peroxide methods. In vitro cytotoxicity and hepatoprotective potential of FSEE were evaluated using HepG2 cells. Based on the cytotoxicity assay, FSEE (50, 100, 200 µg/ml) was assessed for hepatoprotective potential against CCl4 induced toxicity in HepG2 cell line by monitoring cell viability, aspartate aminotransferase (AST), alanine aminotransaminase (ALT), lactate dehydrogenase (LDH) leakage, lipid peroxidation (LPO) and glutathione level (GSH). Further, in vivo hepatoprotective activity of FSEE was evaluated against CCl4 induced hepatotoxicity in male Wistar albino rats. Rats were pre-treated with FSEE (200 mg, 400 mg kg(-1) day(-1) p.o.) for 7 days followed by a single dose of CCl4 (1.0 ml/kg, i.p.) on 8th day. Silymarin was used as positive control. After 24h of CCl4 administration, various biochemical parameters like aspartate aminotransferase (AST), alanine aminotransaminase (ALT), alkaline phosphatase (ALP), total bilirubin (TB) and total protein (TP) levels were estimated in serum. The antioxidant parameters like superoxide dismutase (SOD) activity, catalase (CAT) activity, glutathione (GSH) content and malondialdehyde (MDA) level in the liver homogenate were determined. Histopathological changes in the liver of different groups were also studied. RESULTS: The FSEE possessed strong antioxidant activity in vitro. The results indicated that CCl4 treatment caused a significant decrease in cell viability. The CCl4-induced changes in the HepG2 cells were significantly ameliorated by treatment of the FSEE. FSEE significantly prevented CCl4 induced elevation of AST, ALT, ALP, TB, and CCl4 induced decrease in total protein in rats. FSEE treated rat liver anti-oxidant parameters (SOD, CAT, MDA and GSH,) were significantly antagonized for the pro-oxidant effect of CCl4. Histopathological studies also supported the protective effect of FSEE. CONCLUSION: The results of this study revealed that FSEE has significant hepatoprotective activity. This effect may be due to the ability of the extract to inhibit lipid peroxidation and increase in the anti-oxidant enzymatic activity. SN - 1872-7573 UR - https://www.unboundmedicine.com/medline/citation/24140589/Antioxidant_and_hepatoprotective_activity_of_Fagonia_schweinfurthii__Hadidi__Hadidi_extract_in_carbon_tetrachloride_induced_hepatotoxicity_in_HepG2_cell_line_and_rats_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0378-8741(13)00699-5 DB - PRIME DP - Unbound Medicine ER -