Tags

Type your tag names separated by a space and hit enter

Overexpression of constitutively active OsCPK10 increases Arabidopsis resistance against Pseudomonas syringae pv. tomato and rice resistance against Magnaporthe grisea.
Plant Physiol Biochem. 2013 Dec; 73:202-10.PP

Abstract

Calcium-dependent protein kinases (CDPKs) are crucial calcium sensors involved in plant responses to pathogen infection. Here, we report isolation and functional characterization of the pathogen-responsive rice OsCPK10 gene. The expression of OsCPK10 was strongly induced following treatment with a Magnaporthe grisea elicitor. Kinase activity assay showed that the functional OsCPK10 protein not only autophosphorylated, but also phosphorylated Casein in a calcium-dependent manner. Overexpression of constitutively active OsCPK10 in Arabidopsis enhanced the resistance to infection with Pseudomonas syringae pv. tomato, associated with elevated expression of both SA- and JA-related defense genes. Similarly, transgenic rice plants containing constitutively active OsCPK10 exhibited enhanced resistance to blast fungus M. grisea. The enhanced resistance in the transgenic lines was associated with activated expression of SA- and JA-related defense genes. Collectively, our results indicate that rice OsCPK10 is a crucial regulator in plant immune responses, and that it may regulate disease resistance by activating both SA- and JA-dependent defense responses.

Authors+Show Affiliations

State Key Laboratory of Protein and Plant Gene Research, College of Life Sciences, Peking University, Beijing 100871, China.No affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

24141028

Citation

Fu, Liwen, et al. "Overexpression of Constitutively Active OsCPK10 Increases Arabidopsis Resistance Against Pseudomonas Syringae Pv. Tomato and Rice Resistance Against Magnaporthe Grisea." Plant Physiology and Biochemistry : PPB, vol. 73, 2013, pp. 202-10.
Fu L, Yu X, An C. Overexpression of constitutively active OsCPK10 increases Arabidopsis resistance against Pseudomonas syringae pv. tomato and rice resistance against Magnaporthe grisea. Plant Physiol Biochem. 2013;73:202-10.
Fu, L., Yu, X., & An, C. (2013). Overexpression of constitutively active OsCPK10 increases Arabidopsis resistance against Pseudomonas syringae pv. tomato and rice resistance against Magnaporthe grisea. Plant Physiology and Biochemistry : PPB, 73, 202-10. https://doi.org/10.1016/j.plaphy.2013.10.004
Fu L, Yu X, An C. Overexpression of Constitutively Active OsCPK10 Increases Arabidopsis Resistance Against Pseudomonas Syringae Pv. Tomato and Rice Resistance Against Magnaporthe Grisea. Plant Physiol Biochem. 2013;73:202-10. PubMed PMID: 24141028.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Overexpression of constitutively active OsCPK10 increases Arabidopsis resistance against Pseudomonas syringae pv. tomato and rice resistance against Magnaporthe grisea. AU - Fu,Liwen, AU - Yu,Xiangchun, AU - An,Chengcai, Y1 - 2013/10/11/ PY - 2013/08/02/received PY - 2013/10/02/accepted PY - 2013/10/22/entrez PY - 2013/10/22/pubmed PY - 2014/7/30/medline KW - CDPK KW - Plant immunity SP - 202 EP - 10 JF - Plant physiology and biochemistry : PPB JO - Plant Physiol Biochem VL - 73 N2 - Calcium-dependent protein kinases (CDPKs) are crucial calcium sensors involved in plant responses to pathogen infection. Here, we report isolation and functional characterization of the pathogen-responsive rice OsCPK10 gene. The expression of OsCPK10 was strongly induced following treatment with a Magnaporthe grisea elicitor. Kinase activity assay showed that the functional OsCPK10 protein not only autophosphorylated, but also phosphorylated Casein in a calcium-dependent manner. Overexpression of constitutively active OsCPK10 in Arabidopsis enhanced the resistance to infection with Pseudomonas syringae pv. tomato, associated with elevated expression of both SA- and JA-related defense genes. Similarly, transgenic rice plants containing constitutively active OsCPK10 exhibited enhanced resistance to blast fungus M. grisea. The enhanced resistance in the transgenic lines was associated with activated expression of SA- and JA-related defense genes. Collectively, our results indicate that rice OsCPK10 is a crucial regulator in plant immune responses, and that it may regulate disease resistance by activating both SA- and JA-dependent defense responses. SN - 1873-2690 UR - https://www.unboundmedicine.com/medline/citation/24141028/Overexpression_of_constitutively_active_OsCPK10_increases_Arabidopsis_resistance_against_Pseudomonas_syringae_pv__tomato_and_rice_resistance_against_Magnaporthe_grisea_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0981-9428(13)00350-1 DB - PRIME DP - Unbound Medicine ER -