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Sensitive electrochemical aptasensor for thrombin detection based on graphene served as platform and graphene oxide as enhancer.
Appl Biochem Biotechnol. 2014 Jan; 172(2):1018-26.AB

Abstract

A sensitive electrochemical aptasensor was developed with conductive graphene served as platform and inert graphene oxide (GO) as enhancer. An electrodeposited nano-Au layer was firstly formed on conductive graphene modified glass carbon electrode surface for further immobilizing of electrochemical redox probe hexacyanoferrates nanoparticles (NiHCFNPs). Subsequently, another nano-Au layer was formed for immobilizing of thrombin aptamer (TBA). In the presence of thrombin, the TBA on the electrode surface could bind with thrombin, which made a barrier for electrons and inhibited the electro-transfer, resulting in the decreased electrochemical signals of NiHCFNPs. Owing to the non-conductivity property of graphene oxide, further decreased electrochemical signals of NiHCFNPs could be obtained via the sandwich reaction with GO-labeled TBA. According to the signal changes before the thrombin recognition and after sandwich reaction, trace detection of thrombin could be achieved. As a result, the proposed approach showed a high sensitivity and a wider linearity to thrombin in the range from 0.005 nM to 50 nM with a detection limit of 1 pM.

Authors+Show Affiliations

College of Chemistry and Chemical Engineering, Yibin University, Sichuan, 644000, China.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

24142359

Citation

He, Chun, et al. "Sensitive Electrochemical Aptasensor for Thrombin Detection Based On Graphene Served as Platform and Graphene Oxide as Enhancer." Applied Biochemistry and Biotechnology, vol. 172, no. 2, 2014, pp. 1018-26.
He C, Xu Z, Sun T, et al. Sensitive electrochemical aptasensor for thrombin detection based on graphene served as platform and graphene oxide as enhancer. Appl Biochem Biotechnol. 2014;172(2):1018-26.
He, C., Xu, Z., Sun, T., & Wang, L. (2014). Sensitive electrochemical aptasensor for thrombin detection based on graphene served as platform and graphene oxide as enhancer. Applied Biochemistry and Biotechnology, 172(2), 1018-26. https://doi.org/10.1007/s12010-013-0588-1
He C, et al. Sensitive Electrochemical Aptasensor for Thrombin Detection Based On Graphene Served as Platform and Graphene Oxide as Enhancer. Appl Biochem Biotechnol. 2014;172(2):1018-26. PubMed PMID: 24142359.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Sensitive electrochemical aptasensor for thrombin detection based on graphene served as platform and graphene oxide as enhancer. AU - He,Chun, AU - Xu,Zenghong, AU - Sun,Tao, AU - Wang,Li, Y1 - 2013/10/19/ PY - 2013/09/02/received PY - 2013/10/03/accepted PY - 2013/10/22/entrez PY - 2013/10/22/pubmed PY - 2014/10/8/medline SP - 1018 EP - 26 JF - Applied biochemistry and biotechnology JO - Appl Biochem Biotechnol VL - 172 IS - 2 N2 - A sensitive electrochemical aptasensor was developed with conductive graphene served as platform and inert graphene oxide (GO) as enhancer. An electrodeposited nano-Au layer was firstly formed on conductive graphene modified glass carbon electrode surface for further immobilizing of electrochemical redox probe hexacyanoferrates nanoparticles (NiHCFNPs). Subsequently, another nano-Au layer was formed for immobilizing of thrombin aptamer (TBA). In the presence of thrombin, the TBA on the electrode surface could bind with thrombin, which made a barrier for electrons and inhibited the electro-transfer, resulting in the decreased electrochemical signals of NiHCFNPs. Owing to the non-conductivity property of graphene oxide, further decreased electrochemical signals of NiHCFNPs could be obtained via the sandwich reaction with GO-labeled TBA. According to the signal changes before the thrombin recognition and after sandwich reaction, trace detection of thrombin could be achieved. As a result, the proposed approach showed a high sensitivity and a wider linearity to thrombin in the range from 0.005 nM to 50 nM with a detection limit of 1 pM. SN - 1559-0291 UR - https://www.unboundmedicine.com/medline/citation/24142359/Sensitive_electrochemical_aptasensor_for_thrombin_detection_based_on_graphene_served_as_platform_and_graphene_oxide_as_enhancer_ L2 - https://dx.doi.org/10.1007/s12010-013-0588-1 DB - PRIME DP - Unbound Medicine ER -