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Inhibitory effect of dexamethasone on expression of cysteine-rich 61 protein in airway epithelial cells of allergic mouse models.
J Huazhong Univ Sci Technolog Med Sci. 2013 Oct; 33(5):628-631.JH

Abstract

In order to study whether cysteine-rich 61 protein (cyr61) is involved in the pathogenesis of asthma and its relation to airway inflammation, the effect of dexamethasone (Dxm) on the expression of cyr61 in the lung tissues of asthmatic mice was investigated. Forty BALB/c mice were divided into asthma group (n=15), control group (n=10) and Dxm group (n=15). The asthma group was sensitized and challenged by ovalbumin (OVA). The mice in Dxm group were intraperitoneally administered with Dxm after OVA challenge. The expression of cyr61 in the lung tissues was detected by using immunohistochemistry, and that of eotaxin protein in the bronchoalveolar lavage fluid (BALF) by using enzyme-linked immunosorbent assay (ELISA). The number of inflammatory cells in BALF was also analyzed. The results showed that the cyr61 expression was highest in asthma group (P<0.05), followed by Dxm group (P<0.05) and control group. The cyr61 had a positive correlation with the total nucleated cells (r=0.867, P<0.05), especially eosinophils (r=0.856, P<0.05), and eotaxin level (r=0.983, P<0.05) in the BALF. Our findings suggested that cyr61 is expressed in airway epithelial cells and has a positive correlation with eotaxin and number of airway infiltrating eosinophils.

Authors+Show Affiliations

Department of Respiratory and Critical Care Medicine, Tongji Hospital, Key Laboratory of Pulmonary Diseases of the Ministry of Health of China, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430030, China.Department of Respiratory and Critical Care Medicine, Tongji Hospital, Key Laboratory of Pulmonary Diseases of the Ministry of Health of China, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430030, China.Department of Respiratory and Critical Care Medicine, Tongji Hospital, Key Laboratory of Pulmonary Diseases of the Ministry of Health of China, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430030, China.Department of Respiratory and Critical Care Medicine, Tongji Hospital, Key Laboratory of Pulmonary Diseases of the Ministry of Health of China, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430030, China.Department of Respiratory and Critical Care Medicine, Tongji Hospital, Key Laboratory of Pulmonary Diseases of the Ministry of Health of China, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430030, China.Department of Respiratory and Critical Care Medicine, Tongji Hospital, Key Laboratory of Pulmonary Diseases of the Ministry of Health of China, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430030, China.Department of Respiratory and Critical Care Medicine, Tongji Hospital, Key Laboratory of Pulmonary Diseases of the Ministry of Health of China, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430030, China. fanghuijuan@yahoo.com.cn.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

24142710

Citation

Cao, Yong, et al. "Inhibitory Effect of Dexamethasone On Expression of Cysteine-rich 61 Protein in Airway Epithelial Cells of Allergic Mouse Models." Journal of Huazhong University of Science and Technology. Medical Sciences = Hua Zhong Ke Ji Da Xue Xue Bao. Yi Xue Ying De Wen Ban = Huazhong Keji Daxue Xuebao. Yixue Yingdewen Ban, vol. 33, no. 5, 2013, pp. 628-631.
Cao Y, Chen HL, Cheng S, et al. Inhibitory effect of dexamethasone on expression of cysteine-rich 61 protein in airway epithelial cells of allergic mouse models. J Huazhong Univ Sci Technolog Med Sci. 2013;33(5):628-631.
Cao, Y., Chen, H. L., Cheng, S., Xie, J. G., Xiong, W. N., Xu, Y. J., & Fang, H. J. (2013). Inhibitory effect of dexamethasone on expression of cysteine-rich 61 protein in airway epithelial cells of allergic mouse models. Journal of Huazhong University of Science and Technology. Medical Sciences = Hua Zhong Ke Ji Da Xue Xue Bao. Yi Xue Ying De Wen Ban = Huazhong Keji Daxue Xuebao. Yixue Yingdewen Ban, 33(5), 628-631. https://doi.org/10.1007/s11596-013-1170-3
Cao Y, et al. Inhibitory Effect of Dexamethasone On Expression of Cysteine-rich 61 Protein in Airway Epithelial Cells of Allergic Mouse Models. J Huazhong Univ Sci Technolog Med Sci. 2013;33(5):628-631. PubMed PMID: 24142710.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Inhibitory effect of dexamethasone on expression of cysteine-rich 61 protein in airway epithelial cells of allergic mouse models. AU - Cao,Yong, AU - Chen,Hui-Long, AU - Cheng,Sheng, AU - Xie,Jun-Gang, AU - Xiong,Wei-Ning, AU - Xu,Yong-Jian, AU - Fang,Hui-Juan, Y1 - 2013/10/20/ PY - 2013/06/20/received PY - 2013/08/29/revised PY - 2013/10/22/entrez PY - 2013/10/22/pubmed PY - 2014/10/31/medline SP - 628 EP - 631 JF - Journal of Huazhong University of Science and Technology. Medical sciences = Hua zhong ke ji da xue xue bao. Yi xue Ying De wen ban = Huazhong keji daxue xuebao. Yixue Yingdewen ban JO - J Huazhong Univ Sci Technolog Med Sci VL - 33 IS - 5 N2 - In order to study whether cysteine-rich 61 protein (cyr61) is involved in the pathogenesis of asthma and its relation to airway inflammation, the effect of dexamethasone (Dxm) on the expression of cyr61 in the lung tissues of asthmatic mice was investigated. Forty BALB/c mice were divided into asthma group (n=15), control group (n=10) and Dxm group (n=15). The asthma group was sensitized and challenged by ovalbumin (OVA). The mice in Dxm group were intraperitoneally administered with Dxm after OVA challenge. The expression of cyr61 in the lung tissues was detected by using immunohistochemistry, and that of eotaxin protein in the bronchoalveolar lavage fluid (BALF) by using enzyme-linked immunosorbent assay (ELISA). The number of inflammatory cells in BALF was also analyzed. The results showed that the cyr61 expression was highest in asthma group (P<0.05), followed by Dxm group (P<0.05) and control group. The cyr61 had a positive correlation with the total nucleated cells (r=0.867, P<0.05), especially eosinophils (r=0.856, P<0.05), and eotaxin level (r=0.983, P<0.05) in the BALF. Our findings suggested that cyr61 is expressed in airway epithelial cells and has a positive correlation with eotaxin and number of airway infiltrating eosinophils. SN - 1672-0733 UR - https://www.unboundmedicine.com/medline/citation/24142710/Inhibitory_effect_of_dexamethasone_on_expression_of_cysteine_rich_61_protein_in_airway_epithelial_cells_of_allergic_mouse_models_ L2 - https://doi.org/10.1007/s11596-013-1170-3 DB - PRIME DP - Unbound Medicine ER -