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Downregulation of miR-130a contributes to cisplatin resistance in ovarian cancer cells by targeting X-linked inhibitor of apoptosis (XIAP) directly.
Acta Biochim Biophys Sin (Shanghai). 2013 Dec; 45(12):995-1001.AB

Abstract

MicroRNAs (miRNAs) are short, highly conserved small non-coding RNA molecules, which post-transcriptionally regulate genes expression and play crucial roles in diverse biological processes. Recent studies have shown that dysregulation of miRNAs might modulate the resistance of cancer cells to chemotherapeutic agents. To investigate the possible role of miR-130a in the development of cisplatin resistance in human ovarian cancer cell line A2780, we evaluated the expression of microRNA-130a (miR-130a) in the cells by the quantitative real-time reverse transcription-polymerase chain reaction. The results showed that miR-130a was significantly down-regulated in cisplatin-resistant ovarian cancer cells. MTT assay and flow cytometry (FCM) results showed that over-expression of miR-130a regulated apoptotic activity, and thereby cisplatin chemosensitivity, in ovarian cancer cells. Furthermore, we found that miR-130a can directly target XIAP, and participate in the regulation of apoptosis. The up-regulation of miR-130a led to a significant decrease in the XIAP mRNA levels and protein levels. XIAP plays an important role in cisplatin resistance in ovarian cancer cell line A2780. Our findings suggested that miR-130a could play a role in the development of cisplatin resistance in ovarian cancer cell line A2780, at least in part by modulation of apoptosis via targeting XIAP.

Authors+Show Affiliations

Department of Clinical Laboratory, Xi'an Tradition Chinese Medicine Hospital, Xi'an 710001, China.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

24145606

Citation

Zhang, Xi'an, et al. "Downregulation of miR-130a Contributes to Cisplatin Resistance in Ovarian Cancer Cells By Targeting X-linked Inhibitor of Apoptosis (XIAP) Directly." Acta Biochimica Et Biophysica Sinica, vol. 45, no. 12, 2013, pp. 995-1001.
Zhang X, Huang L, Zhao Y, et al. Downregulation of miR-130a contributes to cisplatin resistance in ovarian cancer cells by targeting X-linked inhibitor of apoptosis (XIAP) directly. Acta Biochim Biophys Sin (Shanghai). 2013;45(12):995-1001.
Zhang, X., Huang, L., Zhao, Y., & Tan, W. (2013). Downregulation of miR-130a contributes to cisplatin resistance in ovarian cancer cells by targeting X-linked inhibitor of apoptosis (XIAP) directly. Acta Biochimica Et Biophysica Sinica, 45(12), 995-1001. https://doi.org/10.1093/abbs/gmt113
Zhang X, et al. Downregulation of miR-130a Contributes to Cisplatin Resistance in Ovarian Cancer Cells By Targeting X-linked Inhibitor of Apoptosis (XIAP) Directly. Acta Biochim Biophys Sin (Shanghai). 2013;45(12):995-1001. PubMed PMID: 24145606.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Downregulation of miR-130a contributes to cisplatin resistance in ovarian cancer cells by targeting X-linked inhibitor of apoptosis (XIAP) directly. AU - Zhang,Xi'an, AU - Huang,Lingna, AU - Zhao,Yinglin, AU - Tan,Wuhong, Y1 - 2013/10/20/ PY - 2013/10/23/entrez PY - 2013/10/23/pubmed PY - 2014/7/26/medline KW - XIAP KW - cisplatin resistance KW - miR-130a KW - ovarian cancer SP - 995 EP - 1001 JF - Acta biochimica et biophysica Sinica JO - Acta Biochim. Biophys. Sin. (Shanghai) VL - 45 IS - 12 N2 - MicroRNAs (miRNAs) are short, highly conserved small non-coding RNA molecules, which post-transcriptionally regulate genes expression and play crucial roles in diverse biological processes. Recent studies have shown that dysregulation of miRNAs might modulate the resistance of cancer cells to chemotherapeutic agents. To investigate the possible role of miR-130a in the development of cisplatin resistance in human ovarian cancer cell line A2780, we evaluated the expression of microRNA-130a (miR-130a) in the cells by the quantitative real-time reverse transcription-polymerase chain reaction. The results showed that miR-130a was significantly down-regulated in cisplatin-resistant ovarian cancer cells. MTT assay and flow cytometry (FCM) results showed that over-expression of miR-130a regulated apoptotic activity, and thereby cisplatin chemosensitivity, in ovarian cancer cells. Furthermore, we found that miR-130a can directly target XIAP, and participate in the regulation of apoptosis. The up-regulation of miR-130a led to a significant decrease in the XIAP mRNA levels and protein levels. XIAP plays an important role in cisplatin resistance in ovarian cancer cell line A2780. Our findings suggested that miR-130a could play a role in the development of cisplatin resistance in ovarian cancer cell line A2780, at least in part by modulation of apoptosis via targeting XIAP. SN - 1745-7270 UR - https://www.unboundmedicine.com/medline/citation/24145606/Downregulation_of_miR_130a_contributes_to_cisplatin_resistance_in_ovarian_cancer_cells_by_targeting_X_linked_inhibitor_of_apoptosis__XIAP__directly_ L2 - https://academic.oup.com/abbs/article-lookup/doi/10.1093/abbs/gmt113 DB - PRIME DP - Unbound Medicine ER -