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Carbon nanotubes reinforced hollow fiber solid phase microextraction for the determination of strychnine and brucine in urine.
Talanta. 2013 Nov 15; 116:188-94.T

Abstract

A mixed matrix membrane (MMM), based on carbon nanotubes (CNTs) and hollow fiber (HF), was prepared and combined with solid phase microextraction (SPME) mode to determine strychnine and brucine in urine. This MMM was prepared by dispersing CNTs in water via surfactant assistance, and then immobilizing CNTs into the pores of HF by capillary forces and sonification. The prepared carbon nanotubes reinforced hollow fiber (CNTs-HF) was subsequently wetted by a few microliters of organic solvent (1-octanol), and then applied to extract the target analytes in direct immersion sampling mode. After extraction, analytes were desorbed via ultrasonic-assisted effect, and then detected via high-performance liquid chromatography (HPLC). To achieve the highest extraction efficiency, main extraction parameters such as the type and amount of surfactant, the diameter and doping level of CNTs, extraction time, desorption condition, pH value, stirring rate and volume of the donor phase were optimized. Under the optimum extraction conditions, the method showed good linearity ranges with correlation coefficients higher than 0.9990, good repeatability and batch-to-batch reproducibility with relative standard deviations (RSDs) less than 6% and 5% for strychnine and brucine, respectively, and low limits of detection (0.7 and 0.9 µg L(-1) for strychnine and brucine, respectively). The recoveries were in the range of 83.81-116.14% at three spiked levels. The developed method was successfully applied to real urine sample with mean relative recoveries of 94.28% and 91.30% for strychnine and brucine, respectively. The developed method shows comparable results against reference methods and is a simple, green, and cost-effective microextraction technique.

Authors+Show Affiliations

Key Laboratory of Chemistry of Northwestern Plant Resources and Key Laboratory for Natural Medicine of Gansu Province, Lanzhou Institute of Chemical Physics, Chinese Academy of Sciences, Lanzhou 730000, PR China; University of Chinese Academy of Sciences, Beijing 100039, PR China.No affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

24148391

Citation

Song, Xin-Yue, et al. "Carbon Nanotubes Reinforced Hollow Fiber Solid Phase Microextraction for the Determination of Strychnine and Brucine in Urine." Talanta, vol. 116, 2013, pp. 188-94.
Song XY, Shi YP, Chen J. Carbon nanotubes reinforced hollow fiber solid phase microextraction for the determination of strychnine and brucine in urine. Talanta. 2013;116:188-94.
Song, X. Y., Shi, Y. P., & Chen, J. (2013). Carbon nanotubes reinforced hollow fiber solid phase microextraction for the determination of strychnine and brucine in urine. Talanta, 116, 188-94. https://doi.org/10.1016/j.talanta.2013.05.025
Song XY, Shi YP, Chen J. Carbon Nanotubes Reinforced Hollow Fiber Solid Phase Microextraction for the Determination of Strychnine and Brucine in Urine. Talanta. 2013 Nov 15;116:188-94. PubMed PMID: 24148391.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Carbon nanotubes reinforced hollow fiber solid phase microextraction for the determination of strychnine and brucine in urine. AU - Song,Xin-Yue, AU - Shi,Yan-Ping, AU - Chen,Juan, Y1 - 2013/05/16/ PY - 2013/03/01/received PY - 2013/05/09/revised PY - 2013/05/11/accepted PY - 2013/10/24/entrez PY - 2013/10/24/pubmed PY - 2014/6/4/medline KW - Brucine KW - CNTs–HF KW - CNTs–HF-SPME KW - HF KW - HF-SPME KW - HPLC KW - HPLC-PDA KW - Hollow fiber KW - MMM KW - MWCNTs KW - Multi-walled carbon nanotubes KW - Solid-phase microextraction KW - Strychnine KW - Surfactant KW - carbon nanotubes reinforced hollow fiber KW - carbon nanotubes reinforced hollow fiber solid-phase microextraction KW - high-performance liquid chromatography KW - high-performance liquid chromatography photodiode array detection KW - hollow fiber KW - hollow fiber solid-phase microextraction KW - mixed matrix membrane KW - multi-walled carbon nanotubes SP - 188 EP - 94 JF - Talanta JO - Talanta VL - 116 N2 - A mixed matrix membrane (MMM), based on carbon nanotubes (CNTs) and hollow fiber (HF), was prepared and combined with solid phase microextraction (SPME) mode to determine strychnine and brucine in urine. This MMM was prepared by dispersing CNTs in water via surfactant assistance, and then immobilizing CNTs into the pores of HF by capillary forces and sonification. The prepared carbon nanotubes reinforced hollow fiber (CNTs-HF) was subsequently wetted by a few microliters of organic solvent (1-octanol), and then applied to extract the target analytes in direct immersion sampling mode. After extraction, analytes were desorbed via ultrasonic-assisted effect, and then detected via high-performance liquid chromatography (HPLC). To achieve the highest extraction efficiency, main extraction parameters such as the type and amount of surfactant, the diameter and doping level of CNTs, extraction time, desorption condition, pH value, stirring rate and volume of the donor phase were optimized. Under the optimum extraction conditions, the method showed good linearity ranges with correlation coefficients higher than 0.9990, good repeatability and batch-to-batch reproducibility with relative standard deviations (RSDs) less than 6% and 5% for strychnine and brucine, respectively, and low limits of detection (0.7 and 0.9 µg L(-1) for strychnine and brucine, respectively). The recoveries were in the range of 83.81-116.14% at three spiked levels. The developed method was successfully applied to real urine sample with mean relative recoveries of 94.28% and 91.30% for strychnine and brucine, respectively. The developed method shows comparable results against reference methods and is a simple, green, and cost-effective microextraction technique. SN - 1873-3573 UR - https://www.unboundmedicine.com/medline/citation/24148391/Carbon_nanotubes_reinforced_hollow_fiber_solid_phase_microextraction_for_the_determination_of_strychnine_and_brucine_in_urine_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0039-9140(13)00448-7 DB - PRIME DP - Unbound Medicine ER -