Tags

Type your tag names separated by a space and hit enter

Ultra-high-performance liquid chromatography profiling method for chemical screening of proanthocyanidins in Czech hops.
Talanta. 2013 Nov 15; 116:919-26.T

Abstract

Hops represent an important natural source of bioactive polyphenols, particularly proanthocyanidins, which can contribute to prevention of several civilization diseases, owing to their antioxidant and radical scavenging activity. We have developed a high-throughput ultra-high-performance liquid chromatography time-of-flight mass spectrometry profiling method, which can be used for monitoring of bioactive proanthocyanidins in hops. The method was applied for analysis of hops of four Czech varieties (Saaz, Sladek, Preminat and Agnus) from the 2011 crop (9 localities, 11 samples) and the 2012 crop (24 localities, 40 samples). Hop samples were extracted by acetone and the analytes were separated on the Acquity UPLC BEH Shield RP18 column. Partial validation of the method revealed a satisfactory intra-day repeatability of the method for retention times (relative standard deviation within 1.39%) as well as areas under the peaks (within 9.89%). Experimental data were evaluated using principal component analysis and cluster analysis. Significant amounts of di-, tri- and tetramer proanthocyanidins consisting of (epi)catechin and (epi)gallocatechin were found in the hop samples. The dependence of the proantocyanidin composition on both the variety and the growing locality was observed. Specifically, the traditional Saaz variety contained more frequently oligomers formed by (epi)catechin units only, whereas the varieties Premiant and Agnus produced oligomers consisting of (epi)catechin as well as (epi)gallocatechin units. The relative abundance of proanthocyanidins in studied hop varieties from the two crops, 2011 and 2012, did correspond to each other. In the further perspective, the method may also be used for prediction of qualitative marks or authenticity verification of hops.

Authors+Show Affiliations

Research Institute of Brewing and Malting PLC, Lípová 15, 120 44 Prague 2, Czech Republic.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

24148495

Citation

Olšovská, J, et al. "Ultra-high-performance Liquid Chromatography Profiling Method for Chemical Screening of Proanthocyanidins in Czech Hops." Talanta, vol. 116, 2013, pp. 919-26.
Olšovská J, Kameník Z, Čejka P, et al. Ultra-high-performance liquid chromatography profiling method for chemical screening of proanthocyanidins in Czech hops. Talanta. 2013;116:919-26.
Olšovská, J., Kameník, Z., Čejka, P., Jurková, M., & Mikyška, A. (2013). Ultra-high-performance liquid chromatography profiling method for chemical screening of proanthocyanidins in Czech hops. Talanta, 116, 919-26. https://doi.org/10.1016/j.talanta.2013.07.067
Olšovská J, et al. Ultra-high-performance Liquid Chromatography Profiling Method for Chemical Screening of Proanthocyanidins in Czech Hops. Talanta. 2013 Nov 15;116:919-26. PubMed PMID: 24148495.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Ultra-high-performance liquid chromatography profiling method for chemical screening of proanthocyanidins in Czech hops. AU - Olšovská,J, AU - Kameník,Z, AU - Čejka,P, AU - Jurková,M, AU - Mikyška,A, Y1 - 2013/08/03/ PY - 2013/02/26/received PY - 2013/07/19/revised PY - 2013/07/26/accepted PY - 2013/10/24/entrez PY - 2013/10/24/pubmed PY - 2014/6/4/medline KW - Catechin KW - Cluster analysis KW - Flavan-3-ol KW - Hops KW - PCA KW - Proanthocyanidins KW - Profiling KW - TOF MS KW - UHPLC SP - 919 EP - 26 JF - Talanta JO - Talanta VL - 116 N2 - Hops represent an important natural source of bioactive polyphenols, particularly proanthocyanidins, which can contribute to prevention of several civilization diseases, owing to their antioxidant and radical scavenging activity. We have developed a high-throughput ultra-high-performance liquid chromatography time-of-flight mass spectrometry profiling method, which can be used for monitoring of bioactive proanthocyanidins in hops. The method was applied for analysis of hops of four Czech varieties (Saaz, Sladek, Preminat and Agnus) from the 2011 crop (9 localities, 11 samples) and the 2012 crop (24 localities, 40 samples). Hop samples were extracted by acetone and the analytes were separated on the Acquity UPLC BEH Shield RP18 column. Partial validation of the method revealed a satisfactory intra-day repeatability of the method for retention times (relative standard deviation within 1.39%) as well as areas under the peaks (within 9.89%). Experimental data were evaluated using principal component analysis and cluster analysis. Significant amounts of di-, tri- and tetramer proanthocyanidins consisting of (epi)catechin and (epi)gallocatechin were found in the hop samples. The dependence of the proantocyanidin composition on both the variety and the growing locality was observed. Specifically, the traditional Saaz variety contained more frequently oligomers formed by (epi)catechin units only, whereas the varieties Premiant and Agnus produced oligomers consisting of (epi)catechin as well as (epi)gallocatechin units. The relative abundance of proanthocyanidins in studied hop varieties from the two crops, 2011 and 2012, did correspond to each other. In the further perspective, the method may also be used for prediction of qualitative marks or authenticity verification of hops. SN - 1873-3573 UR - https://www.unboundmedicine.com/medline/citation/24148495/Ultra_high_performance_liquid_chromatography_profiling_method_for_chemical_screening_of_proanthocyanidins_in_Czech_hops_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0039-9140(13)00640-1 DB - PRIME DP - Unbound Medicine ER -