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Identification and developmental expression of the enzymes responsible for dopamine, histamine, octopamine and serotonin biosynthesis in the copepod crustacean Calanus finmarchicus.
Gen Comp Endocrinol. 2014 Jan 01; 195:28-39.GC

Abstract

Neurochemicals are likely to play key roles in physiological/behavioral control in the copepod crustacean Calanus finmarchicus, the biomass dominant zooplankton for much of the North Atlantic Ocean. Previously, a de novo assembled transcriptome consisting of 206,041 unique sequences was used to characterize the peptidergic signaling systems of Calanus. Here, this assembly was mined for transcripts encoding enzymes involved in amine biosynthesis. Using known Drosophila melanogaster proteins as templates, transcripts encoding putative Calanus homologs of tryptophan-phenylalanine hydroxylase (dopamine, octopamine and serotonin biosynthesis), tyrosine hydroxylase (dopamine biosynthesis), DOPA decarboxylase (dopamine and serotonin biosynthesis), histidine decarboxylase (histamine biosynthesis), tyrosine decarboxylase (octopamine biosynthesis), tyramine β-hydroxylase (octopamine biosynthesis) and tryptophan hydroxylase (serotonin biosynthesis) were identified. Reverse BLAST and domain analyses show that the proteins deduced from these transcripts possess sequence homology to and the structural hallmarks of their respective enzyme families. Developmental profiling revealed a remarkably consistent pattern of expression for all transcripts, with the highest levels of expression typically seen in the early nauplius and early copepodite. These expression patterns suggest roles for amines during development, particularly in the metamorphic transitions from embryo to nauplius and from nauplius to copepodite. Taken collectively, the data presented here lay a strong foundation for future gene-based studies of aminergic signaling in this and other copepod species, in particular assessment of the roles they may play in developmental control.

Authors+Show Affiliations

Békésy Laboratory of Neurobiology, Pacific Biosciences Research Center, University of Hawaii at Manoa, 1993 East-West Road, Honolulu, HI 96822, USA. Electronic address: crabman@pbrc.hawaii.edu.Békésy Laboratory of Neurobiology, Pacific Biosciences Research Center, University of Hawaii at Manoa, 1993 East-West Road, Honolulu, HI 96822, USA.Békésy Laboratory of Neurobiology, Pacific Biosciences Research Center, University of Hawaii at Manoa, 1993 East-West Road, Honolulu, HI 96822, USA.Békésy Laboratory of Neurobiology, Pacific Biosciences Research Center, University of Hawaii at Manoa, 1993 East-West Road, Honolulu, HI 96822, USA.Békésy Laboratory of Neurobiology, Pacific Biosciences Research Center, University of Hawaii at Manoa, 1993 East-West Road, Honolulu, HI 96822, USA.

Pub Type(s)

Journal Article
Research Support, N.I.H., Extramural
Research Support, U.S. Gov't, Non-P.H.S.

Language

eng

PubMed ID

24148657

Citation

Christie, Andrew E., et al. "Identification and Developmental Expression of the Enzymes Responsible for Dopamine, Histamine, Octopamine and Serotonin Biosynthesis in the Copepod Crustacean Calanus Finmarchicus." General and Comparative Endocrinology, vol. 195, 2014, pp. 28-39.
Christie AE, Fontanilla TM, Roncalli V, et al. Identification and developmental expression of the enzymes responsible for dopamine, histamine, octopamine and serotonin biosynthesis in the copepod crustacean Calanus finmarchicus. Gen Comp Endocrinol. 2014;195:28-39.
Christie, A. E., Fontanilla, T. M., Roncalli, V., Cieslak, M. C., & Lenz, P. H. (2014). Identification and developmental expression of the enzymes responsible for dopamine, histamine, octopamine and serotonin biosynthesis in the copepod crustacean Calanus finmarchicus. General and Comparative Endocrinology, 195, 28-39. https://doi.org/10.1016/j.ygcen.2013.10.003
Christie AE, et al. Identification and Developmental Expression of the Enzymes Responsible for Dopamine, Histamine, Octopamine and Serotonin Biosynthesis in the Copepod Crustacean Calanus Finmarchicus. Gen Comp Endocrinol. 2014 Jan 1;195:28-39. PubMed PMID: 24148657.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Identification and developmental expression of the enzymes responsible for dopamine, histamine, octopamine and serotonin biosynthesis in the copepod crustacean Calanus finmarchicus. AU - Christie,Andrew E, AU - Fontanilla,Tiana M, AU - Roncalli,Vittoria, AU - Cieslak,Matthew C, AU - Lenz,Petra H, Y1 - 2013/10/19/ PY - 2013/07/25/received PY - 2013/10/01/revised PY - 2013/10/04/accepted PY - 2013/10/24/entrez PY - 2013/10/24/pubmed PY - 2014/12/17/medline KW - Functional genomics KW - Illumina sequencing KW - de novo transcriptome assembly KW - in silico transcriptome mining SP - 28 EP - 39 JF - General and comparative endocrinology JO - Gen Comp Endocrinol VL - 195 N2 - Neurochemicals are likely to play key roles in physiological/behavioral control in the copepod crustacean Calanus finmarchicus, the biomass dominant zooplankton for much of the North Atlantic Ocean. Previously, a de novo assembled transcriptome consisting of 206,041 unique sequences was used to characterize the peptidergic signaling systems of Calanus. Here, this assembly was mined for transcripts encoding enzymes involved in amine biosynthesis. Using known Drosophila melanogaster proteins as templates, transcripts encoding putative Calanus homologs of tryptophan-phenylalanine hydroxylase (dopamine, octopamine and serotonin biosynthesis), tyrosine hydroxylase (dopamine biosynthesis), DOPA decarboxylase (dopamine and serotonin biosynthesis), histidine decarboxylase (histamine biosynthesis), tyrosine decarboxylase (octopamine biosynthesis), tyramine β-hydroxylase (octopamine biosynthesis) and tryptophan hydroxylase (serotonin biosynthesis) were identified. Reverse BLAST and domain analyses show that the proteins deduced from these transcripts possess sequence homology to and the structural hallmarks of their respective enzyme families. Developmental profiling revealed a remarkably consistent pattern of expression for all transcripts, with the highest levels of expression typically seen in the early nauplius and early copepodite. These expression patterns suggest roles for amines during development, particularly in the metamorphic transitions from embryo to nauplius and from nauplius to copepodite. Taken collectively, the data presented here lay a strong foundation for future gene-based studies of aminergic signaling in this and other copepod species, in particular assessment of the roles they may play in developmental control. SN - 1095-6840 UR - https://www.unboundmedicine.com/medline/citation/24148657/Identification_and_developmental_expression_of_the_enzymes_responsible_for_dopamine_histamine_octopamine_and_serotonin_biosynthesis_in_the_copepod_crustacean_Calanus_finmarchicus_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0016-6480(13)00405-X DB - PRIME DP - Unbound Medicine ER -