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Silencing the glycerol-3-phosphate dehydrogenase gene in Saccharomyces cerevisiae results in more ethanol being produced and less glycerol.
Biotechnol Lett. 2014 Mar; 36(3):523-9.BL

Abstract

Transcription of the gene coding for glycerol-3-phosphate dehydrogenase (GPD1) was repressed in an industrial strain of Saccharomyces cerevisiae using a silencing vector. A fusion fragment containing GPD1 and Kan MX genes was generated by overlap extension PCR, then, the vector, pYES2.0 GPD1/Kan MX, was constructed by inserting the fusion fragment into the S. cerevisiae plasmid, pYES2.0. pYES2.0 GPD1/Kan MX, was linearized by KpnI, transformed into S. cerevisiae using the PEG/LiAc/ssDNA method, and integrated into the S. cerevisiae chromosome. GPD1 silencing gave 20 % less glycerol-3-phosphate dehydrogenase activity, 19 % lower glycerol production, and 9.7 % higher ethanol production compared with the original strain. These findings further the development of industrial S. cerevisiae strains with improved ethanol production and reduced glycerol content for the efficient production of bio-ethanol.

Links

Publisher Full Text (DOI)

Authors+Show Affiliations

He W
College of Life Sciences, Fujian Normal University, Fuzhou, 350108, People's Republic of China, tingtxue@163.com.
Ye S
No affiliation info available
Xue T
No affiliation info available
Xu S
No affiliation info available
Li W
No affiliation info available
Lu J
No affiliation info available
Cao L
No affiliation info available
Ye B
No affiliation info available
Chen Y
No affiliation info available

MeSH

EthanolGene SilencingGlycerolGlycerol-3-Phosphate Dehydrogenase (NAD+)Saccharomyces cerevisiaeSaccharomyces cerevisiae Proteins

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

24150518

Citation

He, Wenjin, et al. "Silencing the Glycerol-3-phosphate Dehydrogenase Gene in Saccharomyces Cerevisiae Results in More Ethanol Being Produced and Less Glycerol." Biotechnology Letters, vol. 36, no. 3, 2014, pp. 523-9.
He W, Ye S, Xue T, et al. Silencing the glycerol-3-phosphate dehydrogenase gene in Saccharomyces cerevisiae results in more ethanol being produced and less glycerol. Biotechnol Lett. 2014;36(3):523-9.
He, W., Ye, S., Xue, T., Xu, S., Li, W., Lu, J., Cao, L., Ye, B., & Chen, Y. (2014). Silencing the glycerol-3-phosphate dehydrogenase gene in Saccharomyces cerevisiae results in more ethanol being produced and less glycerol. Biotechnology Letters, 36(3), 523-9. https://doi.org/10.1007/s10529-013-1375-3
He W, et al. Silencing the Glycerol-3-phosphate Dehydrogenase Gene in Saccharomyces Cerevisiae Results in More Ethanol Being Produced and Less Glycerol. Biotechnol Lett. 2014;36(3):523-9. PubMed PMID: 24150518.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Silencing the glycerol-3-phosphate dehydrogenase gene in Saccharomyces cerevisiae results in more ethanol being produced and less glycerol. AU - He,Wenjin, AU - Ye,Shichao, AU - Xue,Ting, AU - Xu,Shengyan, AU - Li,Weiyan, AU - Lu,Jihua, AU - Cao,Luoyuan, AU - Ye,Bingying, AU - Chen,Youqiang, Y1 - 2013/10/23/ PY - 2013/05/08/received PY - 2013/09/30/accepted PY - 2013/10/24/entrez PY - 2013/10/24/pubmed PY - 2014/11/11/medline SP - 523 EP - 9 JF - Biotechnology letters JO - Biotechnol Lett VL - 36 IS - 3 N2 - Transcription of the gene coding for glycerol-3-phosphate dehydrogenase (GPD1) was repressed in an industrial strain of Saccharomyces cerevisiae using a silencing vector. A fusion fragment containing GPD1 and Kan MX genes was generated by overlap extension PCR, then, the vector, pYES2.0 GPD1/Kan MX, was constructed by inserting the fusion fragment into the S. cerevisiae plasmid, pYES2.0. pYES2.0 GPD1/Kan MX, was linearized by KpnI, transformed into S. cerevisiae using the PEG/LiAc/ssDNA method, and integrated into the S. cerevisiae chromosome. GPD1 silencing gave 20 % less glycerol-3-phosphate dehydrogenase activity, 19 % lower glycerol production, and 9.7 % higher ethanol production compared with the original strain. These findings further the development of industrial S. cerevisiae strains with improved ethanol production and reduced glycerol content for the efficient production of bio-ethanol. SN - 1573-6776 UR - https://www.unboundmedicine.com/medline/citation/24150518/Silencing_the_glycerol_3_phosphate_dehydrogenase_gene_in_Saccharomyces_cerevisiae_results_in_more_ethanol_being_produced_and_less_glycerol_ L2 - https://doi.org/10.1007/s10529-013-1375-3 DB - PRIME DP - Unbound Medicine ER -