Tags

Type your tag names separated by a space and hit enter

Proinflammatory cytokine tumor necrosis factor (TNF)-like weak inducer of apoptosis (TWEAK) suppresses satellite cell self-renewal through inversely modulating Notch and NF-κB signaling pathways.
J Biol Chem. 2013 Dec 06; 288(49):35159-69.JB

Abstract

Satellite cell self-renewal is an essential process to maintaining the robustness of skeletal muscle regenerative capacity. However, extrinsic factors that regulate self-renewal of satellite cells are not well understood. Here, we demonstrate that TWEAK cytokine reduces the proportion of Pax7(+)/MyoD(-) cells (an index of self-renewal) on myofiber explants and represses multiple components of Notch signaling in satellite cell cultures. The number of Pax7(+) cells is significantly increased in skeletal muscle of TWEAK knock-out (KO) mice compared with wild-type in response to injury. Furthermore, Notch signaling is significantly elevated in cultured satellite cells and in regenerating myofibers of TWEAK-KO mice. Forced activation of Notch signaling through overexpression of the Notch1 intracellular domain (N1ICD) rescued the TWEAK-mediated inhibition of satellite cell self-renewal. TWEAK also activates the NF-κB transcription factor in satellite cells and inhibition of NF-κB significantly improved the number of Pax7(+) cells in TWEAK-treated cultures. Furthermore, our results demonstrate that a reciprocal interaction between NF-κB and Notch signaling governs the inhibitory effect of TWEAK on satellite cell self-renewal. Collectively, our study demonstrates that TWEAK suppresses satellite cell self-renewal through activating NF-κB and repressing Notch signaling.

Authors+Show Affiliations

From the Department of Anatomical Sciences and Neurobiology, University of Louisville School of Medicine, Louisville, Kentucky 40202 and.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, N.I.H., Extramural

Language

eng

PubMed ID

24151074

Citation

Ogura, Yuji, et al. "Proinflammatory Cytokine Tumor Necrosis Factor (TNF)-like Weak Inducer of Apoptosis (TWEAK) Suppresses Satellite Cell Self-renewal Through Inversely Modulating Notch and NF-κB Signaling Pathways." The Journal of Biological Chemistry, vol. 288, no. 49, 2013, pp. 35159-69.
Ogura Y, Mishra V, Hindi SM, et al. Proinflammatory cytokine tumor necrosis factor (TNF)-like weak inducer of apoptosis (TWEAK) suppresses satellite cell self-renewal through inversely modulating Notch and NF-κB signaling pathways. J Biol Chem. 2013;288(49):35159-69.
Ogura, Y., Mishra, V., Hindi, S. M., Kuang, S., & Kumar, A. (2013). Proinflammatory cytokine tumor necrosis factor (TNF)-like weak inducer of apoptosis (TWEAK) suppresses satellite cell self-renewal through inversely modulating Notch and NF-κB signaling pathways. The Journal of Biological Chemistry, 288(49), 35159-69. https://doi.org/10.1074/jbc.M113.517300
Ogura Y, et al. Proinflammatory Cytokine Tumor Necrosis Factor (TNF)-like Weak Inducer of Apoptosis (TWEAK) Suppresses Satellite Cell Self-renewal Through Inversely Modulating Notch and NF-κB Signaling Pathways. J Biol Chem. 2013 Dec 6;288(49):35159-69. PubMed PMID: 24151074.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Proinflammatory cytokine tumor necrosis factor (TNF)-like weak inducer of apoptosis (TWEAK) suppresses satellite cell self-renewal through inversely modulating Notch and NF-κB signaling pathways. AU - Ogura,Yuji, AU - Mishra,Vivek, AU - Hindi,Sajedah M, AU - Kuang,Shihuan, AU - Kumar,Ashok, Y1 - 2013/10/22/ PY - 2013/10/24/entrez PY - 2013/10/24/pubmed PY - 2014/2/14/medline KW - Cytokine Action KW - Inflammation KW - NF-κB KW - Notch Pathway KW - Signaling KW - Skeletal Muscle KW - Stem Cells KW - TWEAK, Satellite Cells, Self-renewal, Notch KW - Tumor Necrosis Factor (TNF) SP - 35159 EP - 69 JF - The Journal of biological chemistry JO - J Biol Chem VL - 288 IS - 49 N2 - Satellite cell self-renewal is an essential process to maintaining the robustness of skeletal muscle regenerative capacity. However, extrinsic factors that regulate self-renewal of satellite cells are not well understood. Here, we demonstrate that TWEAK cytokine reduces the proportion of Pax7(+)/MyoD(-) cells (an index of self-renewal) on myofiber explants and represses multiple components of Notch signaling in satellite cell cultures. The number of Pax7(+) cells is significantly increased in skeletal muscle of TWEAK knock-out (KO) mice compared with wild-type in response to injury. Furthermore, Notch signaling is significantly elevated in cultured satellite cells and in regenerating myofibers of TWEAK-KO mice. Forced activation of Notch signaling through overexpression of the Notch1 intracellular domain (N1ICD) rescued the TWEAK-mediated inhibition of satellite cell self-renewal. TWEAK also activates the NF-κB transcription factor in satellite cells and inhibition of NF-κB significantly improved the number of Pax7(+) cells in TWEAK-treated cultures. Furthermore, our results demonstrate that a reciprocal interaction between NF-κB and Notch signaling governs the inhibitory effect of TWEAK on satellite cell self-renewal. Collectively, our study demonstrates that TWEAK suppresses satellite cell self-renewal through activating NF-κB and repressing Notch signaling. SN - 1083-351X UR - https://www.unboundmedicine.com/medline/citation/24151074/Proinflammatory_cytokine_tumor_necrosis_factor__TNF__like_weak_inducer_of_apoptosis__TWEAK__suppresses_satellite_cell_self_renewal_through_inversely_modulating_Notch_and_NF_κB_signaling_pathways_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0021-9258(20)55384-2 DB - PRIME DP - Unbound Medicine ER -