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Identification and characterisation of phenolics in Polygonum capitatum by ultrahigh-performance liquid chromatography with photodiode array detection and tandem mass spectrometry.
Phytochem Anal. 2013 Nov-Dec; 24(6):556-68.PA

Abstract

INTRODUCTION

Polygonum capitatum is a well-known Chinese medicinal plant widely used by the Miao people for the treatment of various urologic disorders. Previous investigations have shown the presence of various types of phenolics. Our ultrahigh-performance liquid chromatography with photodiode array detection and mass spectrometry (UPLC-PDA-MS) analysis indicated that flavonoid glycosides and polyphenolic glycosides were its major constituents and quite a number of phenolic compounds have not yet been identified. Identification or characterisation of the major compounds of this plant will contribute to the scientific understanding of the medicinal plant and the authentication of the plant material and its pharmaceutical preparations.

OBJECTIVE

To develop an efficient method for the identification and structural characterisation of the major compounds present in P. capitatum.

METHODS

Elution of the 70% ethanol extract of P. capitatum by 80% ethanol on a D101 macroporous resin column afforded a phenolics-enriched fraction, separation of which by Sephadex LH-20 column chromatography eluted with absolute ethanol gave a tannin-free phenolic fraction (TFPF). Compounds present in TFPF were identified and structurally characterised by UPLC-PDA-ESI-MS/MS.

RESULTS

An amino acid and 40 phenolics including a number of flavonoid glycosides and polyphenolic glycosides were identified or structurally characterised in TFPF. Among these compounds, four were new and 19 were described in the plant for the first time.

CONCLUSION

The study showed that TFPF of P. capitatum contained flavonoid glycosides and polyphenolic glycosides as its major principles. Polyphenolic glycosides were perhaps the most typical chemical markers of P. capitatum.

Authors+Show Affiliations

Provincial Key Laboratory of Pharmaceutics in Guizhou Province, School of Pharmacy, Guiyang Medical University, 9 Beijing Road, Guiyang, Guizhou, 550004, PR China.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

24154994

Citation

Liao, Shang-Gao, et al. "Identification and Characterisation of Phenolics in Polygonum Capitatum By Ultrahigh-performance Liquid Chromatography With Photodiode Array Detection and Tandem Mass Spectrometry." Phytochemical Analysis : PCA, vol. 24, no. 6, 2013, pp. 556-68.
Liao SG, Zhang LJ, Sun F, et al. Identification and characterisation of phenolics in Polygonum capitatum by ultrahigh-performance liquid chromatography with photodiode array detection and tandem mass spectrometry. Phytochem Anal. 2013;24(6):556-68.
Liao, S. G., Zhang, L. J., Sun, F., Wang, Z., He, X., Wang, A. M., Li, Y. J., Huang, Y., Lan, Y. Y., Zhang, B. L., & Wang, Y. L. (2013). Identification and characterisation of phenolics in Polygonum capitatum by ultrahigh-performance liquid chromatography with photodiode array detection and tandem mass spectrometry. Phytochemical Analysis : PCA, 24(6), 556-68. https://doi.org/10.1002/pca.2432
Liao SG, et al. Identification and Characterisation of Phenolics in Polygonum Capitatum By Ultrahigh-performance Liquid Chromatography With Photodiode Array Detection and Tandem Mass Spectrometry. Phytochem Anal. 2013 Nov-Dec;24(6):556-68. PubMed PMID: 24154994.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Identification and characterisation of phenolics in Polygonum capitatum by ultrahigh-performance liquid chromatography with photodiode array detection and tandem mass spectrometry. AU - Liao,Shang-Gao, AU - Zhang,Li-Juan, AU - Sun,Fa, AU - Wang,Zhen, AU - He,Xun, AU - Wang,Ai-Min, AU - Li,Yong-Jun, AU - Huang,Yong, AU - Lan,Yan-Yu, AU - Zhang,Bo-Li, AU - Wang,Yong-Lin, Y1 - 2013/05/23/ PY - 2012/09/10/received PY - 2013/01/26/revised PY - 2013/01/31/accepted PY - 2013/10/25/entrez PY - 2013/10/25/pubmed PY - 2014/5/30/medline KW - Polygonum capitatum KW - Tandem mass spectrometry KW - UPLC-PDA-MS/MS KW - phenolics SP - 556 EP - 68 JF - Phytochemical analysis : PCA JO - Phytochem Anal VL - 24 IS - 6 N2 - INTRODUCTION: Polygonum capitatum is a well-known Chinese medicinal plant widely used by the Miao people for the treatment of various urologic disorders. Previous investigations have shown the presence of various types of phenolics. Our ultrahigh-performance liquid chromatography with photodiode array detection and mass spectrometry (UPLC-PDA-MS) analysis indicated that flavonoid glycosides and polyphenolic glycosides were its major constituents and quite a number of phenolic compounds have not yet been identified. Identification or characterisation of the major compounds of this plant will contribute to the scientific understanding of the medicinal plant and the authentication of the plant material and its pharmaceutical preparations. OBJECTIVE: To develop an efficient method for the identification and structural characterisation of the major compounds present in P. capitatum. METHODS: Elution of the 70% ethanol extract of P. capitatum by 80% ethanol on a D101 macroporous resin column afforded a phenolics-enriched fraction, separation of which by Sephadex LH-20 column chromatography eluted with absolute ethanol gave a tannin-free phenolic fraction (TFPF). Compounds present in TFPF were identified and structurally characterised by UPLC-PDA-ESI-MS/MS. RESULTS: An amino acid and 40 phenolics including a number of flavonoid glycosides and polyphenolic glycosides were identified or structurally characterised in TFPF. Among these compounds, four were new and 19 were described in the plant for the first time. CONCLUSION: The study showed that TFPF of P. capitatum contained flavonoid glycosides and polyphenolic glycosides as its major principles. Polyphenolic glycosides were perhaps the most typical chemical markers of P. capitatum. SN - 1099-1565 UR - https://www.unboundmedicine.com/medline/citation/24154994/Identification_and_characterisation_of_phenolics_in_Polygonum_capitatum_by_ultrahigh_performance_liquid_chromatography_with_photodiode_array_detection_and_tandem_mass_spectrometry_ L2 - https://doi.org/10.1002/pca.2432 DB - PRIME DP - Unbound Medicine ER -