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Determination of amantadine in biological fluids using simultaneous derivatization and dispersive liquid-liquid microextraction followed by gas chromatography-flame ionization detection.

Abstract

A one-step derivatization and microextraction technique for the determination of amantadine in the human plasma and urine samples is presented. An appropriate mixture of methanol (disperser solvent), 1,2-dibromoethane (extraction solvent), and butylchloroformate (derivatization agent) is rapidly injected into samples. After centrifuging, the sedimented phase is analyzed by gas chromatography-flame ionization detection (GC-FID). The kind of extraction and disperser solvents and their volumes, amount of derivatization agent and reaction/extraction time which are effective in derivatization/dispersive liquid-liquid microextraction (DLLME) procedure are optimized. Under the optimal conditions, the enrichment factor (EF) of the target analyte was obtained to be 408 and 420, and limit of detection (LOD) 4.2 and 2.7ngmL(-1), in plasma and urine respectively. The linear range is 14-5000 and 8.7-5000ng/mL for plasma and urine, respectively (squared correlation coefficient≥0.990). The relative recoveries obtained for the spiked plasma and urine samples are between 72% and 93%. Moreover, the inter- and intra-day precisions are acceptable at all spiked concentrations (relative standard deviation <7%). Finally the method was successfully applied to determine amantadine in biological samples.

Authors+Show Affiliations

Department of Analytical Chemistry, Faculty of Chemistry, University of Tabriz, Tabriz, Iran. Electronic address: mafarajzadeh@yahoo.com.No affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

24157523

Citation

Farajzadeh, Mir Ali, et al. "Determination of Amantadine in Biological Fluids Using Simultaneous Derivatization and Dispersive Liquid-liquid Microextraction Followed By Gas Chromatography-flame Ionization Detection." Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences, vol. 940, 2013, pp. 142-9.
Farajzadeh MA, Nouri N, Alizadeh Nabil AA. Determination of amantadine in biological fluids using simultaneous derivatization and dispersive liquid-liquid microextraction followed by gas chromatography-flame ionization detection. J Chromatogr B Analyt Technol Biomed Life Sci. 2013;940:142-9.
Farajzadeh, M. A., Nouri, N., & Alizadeh Nabil, A. A. (2013). Determination of amantadine in biological fluids using simultaneous derivatization and dispersive liquid-liquid microextraction followed by gas chromatography-flame ionization detection. Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences, 940, 142-9. https://doi.org/10.1016/j.jchromb.2013.09.035
Farajzadeh MA, Nouri N, Alizadeh Nabil AA. Determination of Amantadine in Biological Fluids Using Simultaneous Derivatization and Dispersive Liquid-liquid Microextraction Followed By Gas Chromatography-flame Ionization Detection. J Chromatogr B Analyt Technol Biomed Life Sci. 2013 Dec 1;940:142-9. PubMed PMID: 24157523.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Determination of amantadine in biological fluids using simultaneous derivatization and dispersive liquid-liquid microextraction followed by gas chromatography-flame ionization detection. AU - Farajzadeh,Mir Ali, AU - Nouri,Nina, AU - Alizadeh Nabil,Ali Akbar, Y1 - 2013/10/03/ PY - 2013/04/20/received PY - 2013/09/22/revised PY - 2013/09/26/accepted PY - 2013/10/26/entrez PY - 2013/10/26/pubmed PY - 2014/3/26/medline KW - Amantadine KW - DLLME KW - Derivatization KW - Dispersive liquid–liquid microextraction KW - EF KW - EnF KW - FID KW - GC KW - Gas chromatography KW - HPLC KW - LLE KW - LOD KW - LOQ KW - LPME KW - LR KW - MS KW - dispersive liquid–liquid microextraction KW - enhancement factor KW - enrichment factor KW - flame ionization detection KW - gas chromatography KW - high performance liquid chromatography KW - limit of detection KW - limit of quantification KW - linear range KW - liquid phase microextraction KW - liquid–liquid extraction KW - mass spectrometry SP - 142 EP - 9 JF - Journal of chromatography. B, Analytical technologies in the biomedical and life sciences JO - J Chromatogr B Analyt Technol Biomed Life Sci VL - 940 N2 - A one-step derivatization and microextraction technique for the determination of amantadine in the human plasma and urine samples is presented. An appropriate mixture of methanol (disperser solvent), 1,2-dibromoethane (extraction solvent), and butylchloroformate (derivatization agent) is rapidly injected into samples. After centrifuging, the sedimented phase is analyzed by gas chromatography-flame ionization detection (GC-FID). The kind of extraction and disperser solvents and their volumes, amount of derivatization agent and reaction/extraction time which are effective in derivatization/dispersive liquid-liquid microextraction (DLLME) procedure are optimized. Under the optimal conditions, the enrichment factor (EF) of the target analyte was obtained to be 408 and 420, and limit of detection (LOD) 4.2 and 2.7ngmL(-1), in plasma and urine respectively. The linear range is 14-5000 and 8.7-5000ng/mL for plasma and urine, respectively (squared correlation coefficient≥0.990). The relative recoveries obtained for the spiked plasma and urine samples are between 72% and 93%. Moreover, the inter- and intra-day precisions are acceptable at all spiked concentrations (relative standard deviation <7%). Finally the method was successfully applied to determine amantadine in biological samples. SN - 1873-376X UR - https://www.unboundmedicine.com/medline/citation/24157523/Determination_of_amantadine_in_biological_fluids_using_simultaneous_derivatization_and_dispersive_liquid_liquid_microextraction_followed_by_gas_chromatography_flame_ionization_detection_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S1570-0232(13)00526-6 DB - PRIME DP - Unbound Medicine ER -