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Mast cell-deficient kit mice develop house dust mite-induced lung inflammation despite impaired eosinophil recruitment.
J Innate Immun 2014; 6(2):219-26JI

Abstract

BACKGROUND

Mast cells are implicated in allergic and innate immune responses in asthma, although their role in models using an allergen relevant for human disease is incompletely understood. House dust mite (HDM) allergy is common in asthma patients. Our aim was to investigate the role of mast cells in HDM-induced allergic lung inflammation.

METHODS

Wild-type (Wt) and mast cell-deficient Kit(w-sh) mice on a C57BL/6 background were repetitively exposed to HDM via the airways.

RESULTS

HDM challenge resulted in a rise in tryptase activity in bronchoalveolar lavage fluid (BALF) of Wt mice, indicative of mast cell activation. Kit(w-sh) mice showed a strongly attenuated HDM- induced recruitment of eosinophils in BALF and lung tissue, accompanied by reduced pulmonary levels of the eosinophil chemoattractant eotaxin. Remarkably, Kit(w-sh) mice demonstrated an unaltered capacity to develop lung pathology and increased mucus production in response to HDM. The increased plasma IgE in response to HDM in Wt mice was absent in Kit(w-sh) mice.

CONCLUSION

These data contrast with previous reports on the role of mast cells in models using ovalbumin as allergen in that C57BL/6 Kit(w-sh) mice display a selective impairment of eosinophil recruitment without differences in other features of allergic inflammation.

Authors+Show Affiliations

Center of Infection and Immunity Amsterdam & Center for Experimental and Molecular Medicine, Academic Medical Center, University of Amsterdam, Amsterdam, The Netherlands.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

24157568

Citation

de Boer, J Daan, et al. "Mast Cell-deficient Kit Mice Develop House Dust Mite-induced Lung Inflammation Despite Impaired Eosinophil Recruitment." Journal of Innate Immunity, vol. 6, no. 2, 2014, pp. 219-26.
de Boer JD, Yang J, van den Boogaard FE, et al. Mast cell-deficient kit mice develop house dust mite-induced lung inflammation despite impaired eosinophil recruitment. J Innate Immun. 2014;6(2):219-26.
de Boer, J. D., Yang, J., van den Boogaard, F. E., Hoogendijk, A. J., de Beer, R., van der Zee, J. S., ... van der Poll, T. (2014). Mast cell-deficient kit mice develop house dust mite-induced lung inflammation despite impaired eosinophil recruitment. Journal of Innate Immunity, 6(2), pp. 219-26. doi:10.1159/000354984.
de Boer JD, et al. Mast Cell-deficient Kit Mice Develop House Dust Mite-induced Lung Inflammation Despite Impaired Eosinophil Recruitment. J Innate Immun. 2014;6(2):219-26. PubMed PMID: 24157568.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Mast cell-deficient kit mice develop house dust mite-induced lung inflammation despite impaired eosinophil recruitment. AU - de Boer,J Daan, AU - Yang,Jack, AU - van den Boogaard,Florry E, AU - Hoogendijk,Arie J, AU - de Beer,Regina, AU - van der Zee,Jaring S, AU - Roelofs,Joris J T H, AU - van 't Veer,Cornelis, AU - de Vos,Alex F, AU - van der Poll,Tom, Y1 - 2013/10/18/ PY - 2013/05/01/received PY - 2013/08/12/accepted PY - 2013/10/26/entrez PY - 2013/10/26/pubmed PY - 2015/12/15/medline SP - 219 EP - 26 JF - Journal of innate immunity JO - J Innate Immun VL - 6 IS - 2 N2 - BACKGROUND: Mast cells are implicated in allergic and innate immune responses in asthma, although their role in models using an allergen relevant for human disease is incompletely understood. House dust mite (HDM) allergy is common in asthma patients. Our aim was to investigate the role of mast cells in HDM-induced allergic lung inflammation. METHODS: Wild-type (Wt) and mast cell-deficient Kit(w-sh) mice on a C57BL/6 background were repetitively exposed to HDM via the airways. RESULTS: HDM challenge resulted in a rise in tryptase activity in bronchoalveolar lavage fluid (BALF) of Wt mice, indicative of mast cell activation. Kit(w-sh) mice showed a strongly attenuated HDM- induced recruitment of eosinophils in BALF and lung tissue, accompanied by reduced pulmonary levels of the eosinophil chemoattractant eotaxin. Remarkably, Kit(w-sh) mice demonstrated an unaltered capacity to develop lung pathology and increased mucus production in response to HDM. The increased plasma IgE in response to HDM in Wt mice was absent in Kit(w-sh) mice. CONCLUSION: These data contrast with previous reports on the role of mast cells in models using ovalbumin as allergen in that C57BL/6 Kit(w-sh) mice display a selective impairment of eosinophil recruitment without differences in other features of allergic inflammation. SN - 1662-8128 UR - https://www.unboundmedicine.com/medline/citation/24157568/Mast_cell_deficient_kit_mice_develop_house_dust_mite_induced_lung_inflammation_despite_impaired_eosinophil_recruitment_ L2 - https://www.karger.com?DOI=10.1159/000354984 DB - PRIME DP - Unbound Medicine ER -