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Real-time PCR systems targeting giant viruses of amoebae and their virophages.
Intervirology. 2013; 56(6):413-23.I

Abstract

Giant viruses that infect amoebae, including mimiviruses and marseilleviruses, were first described in 2003. Virophages were subsequently described that infect mimiviruses. Culture isolation with Acanthamoeba spp. and metagenomic studies have shown that these giant viruses are common inhabitants of our biosphere and have enabled the recent detection of these viruses in human samples. However, the genomes of these viruses display substantial genetic diversity, making it a challenge to examine their presence in environmental and clinical samples using conventional and real-time PCR. We designed and evaluated the performance of PCR systems capable of detecting all currently isolated mimiviruses, marseilleviruses and virophages to assess their prevalence in various samples. Our real-time PCR assays accurately detected all or most of the members of the currently delineated lineages of giant viruses infecting acanthamoebae as well as the mimivirus virophages, and enabled accurate classification of the mimiviruses of amoebae in lineages A, B or C. We were able to detect four new mimiviruses directly from environmental samples and correctly classified these viruses within mimivirus lineage C. This was subsequently confirmed by culture on amoebae followed by partial Sanger sequencing. PCR systems such as those implemented here may contribute to an improved understanding of the prevalence of mimiviruses, their virophages and marseilleviruses in humans.

Authors+Show Affiliations

Unité de Recherche sur les Maladies Infectieuses et Tropicales Emergentes (URMITE) UM63, CNRS 7278, IRD 198, INSERM U1095, Facultés de Médecine et de Pharmacie, Aix-Marseille Université, Marseille, France.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Evaluation Study
Journal Article

Language

eng

PubMed ID

24157887

Citation

Ngounga, Tatsiana, et al. "Real-time PCR Systems Targeting Giant Viruses of Amoebae and Their Virophages." Intervirology, vol. 56, no. 6, 2013, pp. 413-23.
Ngounga T, Pagnier I, Reteno DG, et al. Real-time PCR systems targeting giant viruses of amoebae and their virophages. Intervirology. 2013;56(6):413-23.
Ngounga, T., Pagnier, I., Reteno, D. G., Raoult, D., La Scola, B., & Colson, P. (2013). Real-time PCR systems targeting giant viruses of amoebae and their virophages. Intervirology, 56(6), 413-23. https://doi.org/10.1159/000354563
Ngounga T, et al. Real-time PCR Systems Targeting Giant Viruses of Amoebae and Their Virophages. Intervirology. 2013;56(6):413-23. PubMed PMID: 24157887.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Real-time PCR systems targeting giant viruses of amoebae and their virophages. AU - Ngounga,Tatsiana, AU - Pagnier,Isabelle, AU - Reteno,Dorine-Gaelle Ikanga, AU - Raoult,Didier, AU - La Scola,Bernard, AU - Colson,Philippe, Y1 - 2013/10/17/ PY - 2013/10/26/entrez PY - 2013/10/26/pubmed PY - 2014/6/3/medline SP - 413 EP - 23 JF - Intervirology JO - Intervirology VL - 56 IS - 6 N2 - Giant viruses that infect amoebae, including mimiviruses and marseilleviruses, were first described in 2003. Virophages were subsequently described that infect mimiviruses. Culture isolation with Acanthamoeba spp. and metagenomic studies have shown that these giant viruses are common inhabitants of our biosphere and have enabled the recent detection of these viruses in human samples. However, the genomes of these viruses display substantial genetic diversity, making it a challenge to examine their presence in environmental and clinical samples using conventional and real-time PCR. We designed and evaluated the performance of PCR systems capable of detecting all currently isolated mimiviruses, marseilleviruses and virophages to assess their prevalence in various samples. Our real-time PCR assays accurately detected all or most of the members of the currently delineated lineages of giant viruses infecting acanthamoebae as well as the mimivirus virophages, and enabled accurate classification of the mimiviruses of amoebae in lineages A, B or C. We were able to detect four new mimiviruses directly from environmental samples and correctly classified these viruses within mimivirus lineage C. This was subsequently confirmed by culture on amoebae followed by partial Sanger sequencing. PCR systems such as those implemented here may contribute to an improved understanding of the prevalence of mimiviruses, their virophages and marseilleviruses in humans. SN - 1423-0100 UR - https://www.unboundmedicine.com/medline/citation/24157887/Real_time_PCR_systems_targeting_giant_viruses_of_amoebae_and_their_virophages_ L2 - https://www.karger.com?DOI=10.1159/000354563 DB - PRIME DP - Unbound Medicine ER -