Tags

Type your tag names separated by a space and hit enter

Shan virus: a new mimivirus isolated from the stool of a Tunisian patient with pneumonia.
Intervirology. 2013; 56(6):424-9.I

Abstract

OBJECTIVE

Following the isolation of a Marseillevirus from the stool of a healthy young Senegalese and a Mimivirus from a Tunisian patient with pneumonia, we attempted to isolate other giant viruses of amoebae from a large human stool collection.

METHODS

During the period 2010-2011, a total of 1,605 stool samples, including 115 from Tunisian patients with pneumonia, were cultured on amoebae. We used a recently developed high-throughput isolation system to detect amoebae plaque lysis on agar plates; this method allows for the testing of 100 samples per plate per week. The giant virus was identified by sequencing of genes conserved in Megavirales.

RESULTS

A single giant virus, called Shan, was isolated from the stool of a Tunisian patient with pneumonia who responded poorly to antibiotics. This virus has an icosahedral shape typical of members of the family Mimiviridae and a size of 640 ± 10 nm. Phylogenetic analyses showed that Shan virus was classified as a member of Mimivirus lineage C that infects amoebae.

CONCLUSION

Only one isolate was obtained in this study, suggesting that giant viruses of amoebae are rare in human stool. The isolation of Shan virus from a patient with pneumonia brings into question the etiological role of this virus and its subsequent release in stool.

Authors+Show Affiliations

Unité de Recherche sur les Maladies Infectieuses et Tropicales Emergentes (URMITE), Aix-Marseille Université, Marseille, France.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Case Reports
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

24157888

Citation

Saadi, Hanene, et al. "Shan Virus: a New Mimivirus Isolated From the Stool of a Tunisian Patient With Pneumonia." Intervirology, vol. 56, no. 6, 2013, pp. 424-9.
Saadi H, Reteno DG, Colson P, et al. Shan virus: a new mimivirus isolated from the stool of a Tunisian patient with pneumonia. Intervirology. 2013;56(6):424-9.
Saadi, H., Reteno, D. G., Colson, P., Aherfi, S., Minodier, P., Pagnier, I., Raoult, D., & La Scola, B. (2013). Shan virus: a new mimivirus isolated from the stool of a Tunisian patient with pneumonia. Intervirology, 56(6), 424-9. https://doi.org/10.1159/000354564
Saadi H, et al. Shan Virus: a New Mimivirus Isolated From the Stool of a Tunisian Patient With Pneumonia. Intervirology. 2013;56(6):424-9. PubMed PMID: 24157888.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Shan virus: a new mimivirus isolated from the stool of a Tunisian patient with pneumonia. AU - Saadi,Hanene, AU - Reteno,Dorine-Gaelle Ikanga, AU - Colson,Philippe, AU - Aherfi,Sarah, AU - Minodier,Philippe, AU - Pagnier,Isabelle, AU - Raoult,Didier, AU - La Scola,Bernard, Y1 - 2013/10/17/ PY - 2013/10/26/entrez PY - 2013/10/26/pubmed PY - 2014/6/3/medline SP - 424 EP - 9 JF - Intervirology JO - Intervirology VL - 56 IS - 6 N2 - OBJECTIVE: Following the isolation of a Marseillevirus from the stool of a healthy young Senegalese and a Mimivirus from a Tunisian patient with pneumonia, we attempted to isolate other giant viruses of amoebae from a large human stool collection. METHODS: During the period 2010-2011, a total of 1,605 stool samples, including 115 from Tunisian patients with pneumonia, were cultured on amoebae. We used a recently developed high-throughput isolation system to detect amoebae plaque lysis on agar plates; this method allows for the testing of 100 samples per plate per week. The giant virus was identified by sequencing of genes conserved in Megavirales. RESULTS: A single giant virus, called Shan, was isolated from the stool of a Tunisian patient with pneumonia who responded poorly to antibiotics. This virus has an icosahedral shape typical of members of the family Mimiviridae and a size of 640 ± 10 nm. Phylogenetic analyses showed that Shan virus was classified as a member of Mimivirus lineage C that infects amoebae. CONCLUSION: Only one isolate was obtained in this study, suggesting that giant viruses of amoebae are rare in human stool. The isolation of Shan virus from a patient with pneumonia brings into question the etiological role of this virus and its subsequent release in stool. SN - 1423-0100 UR - https://www.unboundmedicine.com/medline/citation/24157888/Shan_virus:_a_new_mimivirus_isolated_from_the_stool_of_a_Tunisian_patient_with_pneumonia_ L2 - https://www.karger.com?DOI=10.1159/000354564 DB - PRIME DP - Unbound Medicine ER -