Tags

Type your tag names separated by a space and hit enter

Natural history of mesenchymal stem cells, from vessel walls to culture vessels.
Cell Mol Life Sci 2014; 71(8):1353-74CM

Abstract

Mesenchymal stem/stromal cells (MSCs) can regenerate tissues by direct differentiation or indirectly by stimulating angiogenesis, limiting inflammation, and recruiting tissue-specific progenitor cells. MSCs emerge and multiply in long-term cultures of total cells from the bone marrow or multiple other organs. Such a derivation in vitro is simple and convenient, hence popular, but has long precluded understanding of the native identity, tissue distribution, frequency, and natural role of MSCs, which have been defined and validated exclusively in terms of surface marker expression and developmental potential in culture into bone, cartilage, and fat. Such simple, widely accepted criteria uniformly typify MSCs, even though some differences in potential exist, depending on tissue sources. Combined immunohistochemistry, flow cytometry, and cell culture have allowed tracking the artifactual cultured mesenchymal stem/stromal cells back to perivascular anatomical regions. Presently, both pericytes enveloping microvessels and adventitial cells surrounding larger arteries and veins have been described as possible MSC forerunners. While such a vascular association would explain why MSCs have been isolated from virtually all tissues tested, the origin of the MSCs grown from umbilical cord blood remains unknown. In fact, most aspects of the biology of perivascular MSCs are still obscure, from the emergence of these cells in the embryo to the molecular control of their activity in adult tissues. Such dark areas have not compromised intents to use these cells in clinical settings though, in which purified perivascular cells already exhibit decisive advantages over conventional MSCs, including purity, thorough characterization and, principally, total independence from in vitro culture. A growing body of experimental data is currently paving the way to the medical usage of autologous sorted perivascular cells for indications in which MSCs have been previously contemplated or actually used, such as bone regeneration and cardiovascular tissue repair.

Authors+Show Affiliations

MRC Center for Regenerative Medicine, University of Edinburgh, Edinburgh, UK.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't
Review

Language

eng

PubMed ID

24158496

Citation

Murray, Iain R., et al. "Natural History of Mesenchymal Stem Cells, From Vessel Walls to Culture Vessels." Cellular and Molecular Life Sciences : CMLS, vol. 71, no. 8, 2014, pp. 1353-74.
Murray IR, West CC, Hardy WR, et al. Natural history of mesenchymal stem cells, from vessel walls to culture vessels. Cell Mol Life Sci. 2014;71(8):1353-74.
Murray, I. R., West, C. C., Hardy, W. R., James, A. W., Park, T. S., Nguyen, A., ... Péault, B. (2014). Natural history of mesenchymal stem cells, from vessel walls to culture vessels. Cellular and Molecular Life Sciences : CMLS, 71(8), pp. 1353-74. doi:10.1007/s00018-013-1462-6.
Murray IR, et al. Natural History of Mesenchymal Stem Cells, From Vessel Walls to Culture Vessels. Cell Mol Life Sci. 2014;71(8):1353-74. PubMed PMID: 24158496.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Natural history of mesenchymal stem cells, from vessel walls to culture vessels. AU - Murray,Iain R, AU - West,Christopher C, AU - Hardy,Winters R, AU - James,Aaron W, AU - Park,Tea Soon, AU - Nguyen,Alan, AU - Tawonsawatruk,Tulyapruek, AU - Lazzari,Lorenza, AU - Soo,Chia, AU - Péault,Bruno, Y1 - 2013/10/25/ PY - 2013/07/06/received PY - 2013/08/23/accepted PY - 2013/08/17/revised PY - 2013/10/26/entrez PY - 2013/10/26/pubmed PY - 2014/5/9/medline SP - 1353 EP - 74 JF - Cellular and molecular life sciences : CMLS JO - Cell. Mol. Life Sci. VL - 71 IS - 8 N2 - Mesenchymal stem/stromal cells (MSCs) can regenerate tissues by direct differentiation or indirectly by stimulating angiogenesis, limiting inflammation, and recruiting tissue-specific progenitor cells. MSCs emerge and multiply in long-term cultures of total cells from the bone marrow or multiple other organs. Such a derivation in vitro is simple and convenient, hence popular, but has long precluded understanding of the native identity, tissue distribution, frequency, and natural role of MSCs, which have been defined and validated exclusively in terms of surface marker expression and developmental potential in culture into bone, cartilage, and fat. Such simple, widely accepted criteria uniformly typify MSCs, even though some differences in potential exist, depending on tissue sources. Combined immunohistochemistry, flow cytometry, and cell culture have allowed tracking the artifactual cultured mesenchymal stem/stromal cells back to perivascular anatomical regions. Presently, both pericytes enveloping microvessels and adventitial cells surrounding larger arteries and veins have been described as possible MSC forerunners. While such a vascular association would explain why MSCs have been isolated from virtually all tissues tested, the origin of the MSCs grown from umbilical cord blood remains unknown. In fact, most aspects of the biology of perivascular MSCs are still obscure, from the emergence of these cells in the embryo to the molecular control of their activity in adult tissues. Such dark areas have not compromised intents to use these cells in clinical settings though, in which purified perivascular cells already exhibit decisive advantages over conventional MSCs, including purity, thorough characterization and, principally, total independence from in vitro culture. A growing body of experimental data is currently paving the way to the medical usage of autologous sorted perivascular cells for indications in which MSCs have been previously contemplated or actually used, such as bone regeneration and cardiovascular tissue repair. SN - 1420-9071 UR - https://www.unboundmedicine.com/medline/citation/24158496/Natural_history_of_mesenchymal_stem_cells_from_vessel_walls_to_culture_vessels_ L2 - https://dx.doi.org/10.1007/s00018-013-1462-6 DB - PRIME DP - Unbound Medicine ER -