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Control of primer formation for ColE1 plasmid replication: conformational change of the primer transcript.
Cell. 1986 Jan 17; 44(1):125-36.Cell

Abstract

The ColE1 primer transcript (RNA II) hybridizes to the template DNA near the replication origin. The hybridized RNA II is cleaved by RNAase H to form the primer. Hybridization is inhibited by binding of another RNA, RNA I, to RNA II. This binding alters the secondary structure of RNA II even in the region far downstream of the segment where RNA I binds. Similar functional and structural alterations of RNA II are produced by a single base-change within this segment. However, a region of about 200 nucleotides proximal to the 5' end of RNA II that includes the segment can be deleted with little effect on primer formation. By forming an alternative structure, the dispensable region affects folding of a downstream region, and thus participates in regulation of primer formation.

Authors

No affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

2416472

Citation

Masukata, H, and J Tomizawa. "Control of Primer Formation for ColE1 Plasmid Replication: Conformational Change of the Primer Transcript." Cell, vol. 44, no. 1, 1986, pp. 125-36.
Masukata H, Tomizawa J. Control of primer formation for ColE1 plasmid replication: conformational change of the primer transcript. Cell. 1986;44(1):125-36.
Masukata, H., & Tomizawa, J. (1986). Control of primer formation for ColE1 plasmid replication: conformational change of the primer transcript. Cell, 44(1), 125-36.
Masukata H, Tomizawa J. Control of Primer Formation for ColE1 Plasmid Replication: Conformational Change of the Primer Transcript. Cell. 1986 Jan 17;44(1):125-36. PubMed PMID: 2416472.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Control of primer formation for ColE1 plasmid replication: conformational change of the primer transcript. AU - Masukata,H, AU - Tomizawa,J, PY - 1986/1/17/pubmed PY - 1986/1/17/medline PY - 1986/1/17/entrez SP - 125 EP - 36 JF - Cell JO - Cell VL - 44 IS - 1 N2 - The ColE1 primer transcript (RNA II) hybridizes to the template DNA near the replication origin. The hybridized RNA II is cleaved by RNAase H to form the primer. Hybridization is inhibited by binding of another RNA, RNA I, to RNA II. This binding alters the secondary structure of RNA II even in the region far downstream of the segment where RNA I binds. Similar functional and structural alterations of RNA II are produced by a single base-change within this segment. However, a region of about 200 nucleotides proximal to the 5' end of RNA II that includes the segment can be deleted with little effect on primer formation. By forming an alternative structure, the dispensable region affects folding of a downstream region, and thus participates in regulation of primer formation. SN - 0092-8674 UR - https://www.unboundmedicine.com/medline/citation/2416472/Control_of_primer_formation_for_ColE1_plasmid_replication:_conformational_change_of_the_primer_transcript_ L2 - https://linkinghub.elsevier.com/retrieve/pii/0092-8674(86)90491-5 DB - PRIME DP - Unbound Medicine ER -