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Detection of Leptospira-specific antibodies using a recombinant antigen-based enzyme-linked immunosorbent assay.
Am J Trop Med Hyg. 2013 Dec; 89(6):1088-94.AJ

Abstract

We produced three highly purified recombinant antigens rLipL32, rLipL41, and rLigA-Rep (leptospiral immunoglobulin-like A repeat region) for the detection of Leptospira-specific antibodies in an enzyme-linked immunosorbent assay (ELISA). The performance of these recombinant antigens was evaluated using 121 human sera. Among them, 63 sera were microscopic agglutination test (MAT)-confirmed positive sera from febrile patients in Peru, 22 sera were indigenous MAT-negative febrile patient sera, and 36 sera were from patients with other febrile diseases from Southeast Asia, where leptospirosis is also endemic. Combining the results of immunoglobulin M (IgM) and IgG detection from these three antigens, the overall sensitivity is close to 90% based on the MAT. These results suggest that an ELISA using multiple recombinant antigens may be used as an alternative method for the detection of Leptospira-specific antibodies.

Authors+Show Affiliations

Naval Medical Research Center, Silver Spring, Maryland; Naval Medical Research Unit No.6, Lima, Peru; Naval Medical Center San Diego, San Diego, California.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, U.S. Gov't, Non-P.H.S.

Language

eng

PubMed ID

24166046

Citation

Chen, Hua-Wei, et al. "Detection of Leptospira-specific Antibodies Using a Recombinant Antigen-based Enzyme-linked Immunosorbent Assay." The American Journal of Tropical Medicine and Hygiene, vol. 89, no. 6, 2013, pp. 1088-94.
Chen HW, Zhang Z, Halsey ES, et al. Detection of Leptospira-specific antibodies using a recombinant antigen-based enzyme-linked immunosorbent assay. Am J Trop Med Hyg. 2013;89(6):1088-94.
Chen, H. W., Zhang, Z., Halsey, E. S., Guevara, C., Canal, E., Hall, E., Maves, R., Tilley, D. H., Kochel, T. J., & Ching, W. M. (2013). Detection of Leptospira-specific antibodies using a recombinant antigen-based enzyme-linked immunosorbent assay. The American Journal of Tropical Medicine and Hygiene, 89(6), 1088-94. https://doi.org/10.4269/ajtmh.13-0041
Chen HW, et al. Detection of Leptospira-specific Antibodies Using a Recombinant Antigen-based Enzyme-linked Immunosorbent Assay. Am J Trop Med Hyg. 2013;89(6):1088-94. PubMed PMID: 24166046.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Detection of Leptospira-specific antibodies using a recombinant antigen-based enzyme-linked immunosorbent assay. AU - Chen,Hua-Wei, AU - Zhang,Zhiwen, AU - Halsey,Eric S, AU - Guevara,Carolina, AU - Canal,Enrique, AU - Hall,Eric, AU - Maves,Ryan, AU - Tilley,Drake H, AU - Kochel,Tadeusz J, AU - Ching,Wei-Mei, Y1 - 2013/10/28/ PY - 2013/10/30/entrez PY - 2013/10/30/pubmed PY - 2014/2/27/medline SP - 1088 EP - 94 JF - The American journal of tropical medicine and hygiene JO - Am J Trop Med Hyg VL - 89 IS - 6 N2 - We produced three highly purified recombinant antigens rLipL32, rLipL41, and rLigA-Rep (leptospiral immunoglobulin-like A repeat region) for the detection of Leptospira-specific antibodies in an enzyme-linked immunosorbent assay (ELISA). The performance of these recombinant antigens was evaluated using 121 human sera. Among them, 63 sera were microscopic agglutination test (MAT)-confirmed positive sera from febrile patients in Peru, 22 sera were indigenous MAT-negative febrile patient sera, and 36 sera were from patients with other febrile diseases from Southeast Asia, where leptospirosis is also endemic. Combining the results of immunoglobulin M (IgM) and IgG detection from these three antigens, the overall sensitivity is close to 90% based on the MAT. These results suggest that an ELISA using multiple recombinant antigens may be used as an alternative method for the detection of Leptospira-specific antibodies. SN - 1476-1645 UR - https://www.unboundmedicine.com/medline/citation/24166046/Detection_of_Leptospira_specific_antibodies_using_a_recombinant_antigen_based_enzyme_linked_immunosorbent_assay_ L2 - https://ajtmh.org/doi/10.4269/ajtmh.13-0041 DB - PRIME DP - Unbound Medicine ER -