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Extraction, stability, and separation of betalains from Opuntia joconostle cv. using response surface methodology.
J Agric Food Chem 2013; 61(49):11995-2004JA

Abstract

Betalains were extracted and analyzed from Opuntia joconostle (the prickly pear known as xoconostle in Mexico). For the extraction, two solvent systems were used, methanol/water and ethanol/water. A three-variable Box-Behnken statistical design was used for extraction: solvent concentration (0-80%, v/v), temperature (5-30 °C), and treatment time (10-30 min). The extraction and stability of betalains from xoconostle were studied using response surface methodology (RSM). Techniques such as UV-vis, column chromatography, and HPLC were employed for the separation and analysis of the main pigments present in the extracts. Maximum pigment concentration (92 mg/100 g of fruit) was obtained at a temperature of 15 °C and a time of 10 min for methanol/water (20:80), whereas maximum stability of the pigment was observed at pH 5 and a temperature of 25 °C. HPLC chromatograms showed the main betalains of the xoconostle characterized were betalain, betanidin, and isobetalain.

Authors+Show Affiliations

Centro de Investigación en Ciencia Aplicada y Tecnologı́a Avanzada del Instituto Politécnico Nacional, Legaria 694, Col. Irrigación, México D.F. C.P. 11500, Mexico.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

24168389

Citation

Sanchez-Gonzalez, Noe, et al. "Extraction, Stability, and Separation of Betalains From Opuntia Joconostle Cv. Using Response Surface Methodology." Journal of Agricultural and Food Chemistry, vol. 61, no. 49, 2013, pp. 11995-2004.
Sanchez-Gonzalez N, Jaime-Fonseca MR, San Martin-Martinez E, et al. Extraction, stability, and separation of betalains from Opuntia joconostle cv. using response surface methodology. J Agric Food Chem. 2013;61(49):11995-2004.
Sanchez-Gonzalez, N., Jaime-Fonseca, M. R., San Martin-Martinez, E., & Zepeda, L. G. (2013). Extraction, stability, and separation of betalains from Opuntia joconostle cv. using response surface methodology. Journal of Agricultural and Food Chemistry, 61(49), pp. 11995-2004. doi:10.1021/jf401705h.
Sanchez-Gonzalez N, et al. Extraction, Stability, and Separation of Betalains From Opuntia Joconostle Cv. Using Response Surface Methodology. J Agric Food Chem. 2013 Dec 11;61(49):11995-2004. PubMed PMID: 24168389.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Extraction, stability, and separation of betalains from Opuntia joconostle cv. using response surface methodology. AU - Sanchez-Gonzalez,Noe, AU - Jaime-Fonseca,Monica R, AU - San Martin-Martinez,Eduardo, AU - Zepeda,L Gerardo, Y1 - 2013/11/23/ PY - 2013/10/31/entrez PY - 2013/10/31/pubmed PY - 2014/7/10/medline SP - 11995 EP - 2004 JF - Journal of agricultural and food chemistry JO - J. Agric. Food Chem. VL - 61 IS - 49 N2 - Betalains were extracted and analyzed from Opuntia joconostle (the prickly pear known as xoconostle in Mexico). For the extraction, two solvent systems were used, methanol/water and ethanol/water. A three-variable Box-Behnken statistical design was used for extraction: solvent concentration (0-80%, v/v), temperature (5-30 °C), and treatment time (10-30 min). The extraction and stability of betalains from xoconostle were studied using response surface methodology (RSM). Techniques such as UV-vis, column chromatography, and HPLC were employed for the separation and analysis of the main pigments present in the extracts. Maximum pigment concentration (92 mg/100 g of fruit) was obtained at a temperature of 15 °C and a time of 10 min for methanol/water (20:80), whereas maximum stability of the pigment was observed at pH 5 and a temperature of 25 °C. HPLC chromatograms showed the main betalains of the xoconostle characterized were betalain, betanidin, and isobetalain. SN - 1520-5118 UR - https://www.unboundmedicine.com/medline/citation/24168389/Extraction_stability_and_separation_of_betalains_from_Opuntia_joconostle_cv__using_response_surface_methodology_ L2 - https://dx.doi.org/10.1021/jf401705h DB - PRIME DP - Unbound Medicine ER -