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Evidence of surfactant protein A and D expression decrement and their localizations in human prostate adenocarcinomas.
Ren Fail. 2014 Mar; 36(2):258-65.RF

Abstract

AIM

Surfactant proteins (SP-A and SP-D) were originally described in the lung; however, they are also present in the prostate. Purpose of this study, therefore, was to determine how surfactant proteins are altered in prostate adenocarcinomas (PCa) and find out any connection exists between their expressions and their staining patterns, prostate-specific antigen (PSA) values, Gleason score, age, tumor volume and tumor, node, metastases (TNM) clinical stage.

METHODS

Thirty-five tissue samples were obtained during radical prostatectomy. All specimens were classified to three groups based on the Gleason score <7, 7 and Gleason score >7. Surfactant proteins' expressions were tested by immunohistochemical and Western blotting methods.

RESULTS

Immunoreactivity was detected in the cytoplasm from both basal cells and secretory epithelial cells in malignant and non-malignant areas. About 80% of the malignant basal cells were characterized as either weak or strong while non-malignant epithelial cells demonstrated strong immunoreactivity for SP-A. Also malignant (81.8%) and non-malignant cells (90.6%) were characterized as either weak or strong for SP-D. Decrement of SP-A and SP-D immunostaining tended to associate with an increasing Gleason score (p > 0.05, p < 0.05), tumor volume (p < 0.05, p > 0.05) and age (p > 0.05, p > 0.05). There was a strong positive correlation between Gleason score and tumor volume (p < 0.01). Also, either none or weak SP-A and SP-D immunoreactivity was observed specimens with Gleason score 7 or higher. SP-A and SP-D reacted with 34 kDa (SP-A) and 43 kDa (SP-D) immunoreactive single bands were decreased in tumor tissues.

CONCLUSIONS

The development of prostate cancer may be related to decreased level of surfactant protein A and D.

Authors+Show Affiliations

Department of Biochemistry, Faculty of Veterinary Medicine, The University of Mehmet Akif Ersoy , Burdur , Turkey .No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

24168624

Citation

Kankavi, Orhan, et al. "Evidence of Surfactant Protein a and D Expression Decrement and Their Localizations in Human Prostate Adenocarcinomas." Renal Failure, vol. 36, no. 2, 2014, pp. 258-65.
Kankavi O, Baykara M, Eren Karanis MI, et al. Evidence of surfactant protein A and D expression decrement and their localizations in human prostate adenocarcinomas. Ren Fail. 2014;36(2):258-65.
Kankavi, O., Baykara, M., Eren Karanis, M. I., Bassorgun, C. I., Ergin, H., & Ciftcioglu, M. A. (2014). Evidence of surfactant protein A and D expression decrement and their localizations in human prostate adenocarcinomas. Renal Failure, 36(2), 258-65. https://doi.org/10.3109/0886022X.2013.846831
Kankavi O, et al. Evidence of Surfactant Protein a and D Expression Decrement and Their Localizations in Human Prostate Adenocarcinomas. Ren Fail. 2014;36(2):258-65. PubMed PMID: 24168624.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Evidence of surfactant protein A and D expression decrement and their localizations in human prostate adenocarcinomas. AU - Kankavi,Orhan, AU - Baykara,Mehmet, AU - Eren Karanis,Meryem Ilkay, AU - Bassorgun,Cumhur Ibrahim, AU - Ergin,Hale, AU - Ciftcioglu,Mehmet Akif, Y1 - 2013/10/29/ PY - 2013/10/31/entrez PY - 2013/10/31/pubmed PY - 2014/10/28/medline SP - 258 EP - 65 JF - Renal failure JO - Ren Fail VL - 36 IS - 2 N2 - AIM: Surfactant proteins (SP-A and SP-D) were originally described in the lung; however, they are also present in the prostate. Purpose of this study, therefore, was to determine how surfactant proteins are altered in prostate adenocarcinomas (PCa) and find out any connection exists between their expressions and their staining patterns, prostate-specific antigen (PSA) values, Gleason score, age, tumor volume and tumor, node, metastases (TNM) clinical stage. METHODS: Thirty-five tissue samples were obtained during radical prostatectomy. All specimens were classified to three groups based on the Gleason score <7, 7 and Gleason score >7. Surfactant proteins' expressions were tested by immunohistochemical and Western blotting methods. RESULTS: Immunoreactivity was detected in the cytoplasm from both basal cells and secretory epithelial cells in malignant and non-malignant areas. About 80% of the malignant basal cells were characterized as either weak or strong while non-malignant epithelial cells demonstrated strong immunoreactivity for SP-A. Also malignant (81.8%) and non-malignant cells (90.6%) were characterized as either weak or strong for SP-D. Decrement of SP-A and SP-D immunostaining tended to associate with an increasing Gleason score (p > 0.05, p < 0.05), tumor volume (p < 0.05, p > 0.05) and age (p > 0.05, p > 0.05). There was a strong positive correlation between Gleason score and tumor volume (p < 0.01). Also, either none or weak SP-A and SP-D immunoreactivity was observed specimens with Gleason score 7 or higher. SP-A and SP-D reacted with 34 kDa (SP-A) and 43 kDa (SP-D) immunoreactive single bands were decreased in tumor tissues. CONCLUSIONS: The development of prostate cancer may be related to decreased level of surfactant protein A and D. SN - 1525-6049 UR - https://www.unboundmedicine.com/medline/citation/24168624/Evidence_of_surfactant_protein_A_and_D_expression_decrement_and_their_localizations_in_human_prostate_adenocarcinomas_ L2 - http://www.tandfonline.com/doi/full/10.3109/0886022X.2013.846831 DB - PRIME DP - Unbound Medicine ER -