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Co-expression of monodehydroascorbate reductase and dehydroascorbate reductase from Brassica rapa effectively confers tolerance to freezing-induced oxidative stress.
Mol Cells. 2013 Oct; 36(4):304-15.MC

Abstract

Plants are exposed to various environmental stresses and have therefore developed antioxidant enzymes and molecules to protect their cellular components against toxicity derived from reactive oxygen species (ROS). Ascorbate is a very important antioxidant molecule in plants, and monodehydroascorbate reductase (MDHAR; EC 1.6.5.4) and dehydroascorbate reductase (DHAR; EC 1.8.5.1) are essential to regeneration of ascorbate for maintenance of ROS scavenging ability. The MDHAR and DHAR genes from Brassica rapa were cloned, transgenic plants overexpressing either BrMDHAR and BrDHAR were established, and then, each transgenic plant was hybridized to examine the effects of co-expression of both genes conferring tolerance to freezing. Transgenic plants co-overexpressing BrMDHAR and BrDHAR showed activated expression of relative antioxidant enzymes, and enhanced levels of glutathione and phenolics under freezing condition. Then, these alteration caused by co-expression led to alleviated redox status and lipid peroxidation and consequently conferred improved tolerance against severe freezing stress compared to transgenic plants overexpressing single gene. The results of this study suggested that although each expression of BrMDHAR or BrDHAR was available to according tolerance to freezing, the simultaneous expression of two genes generated synergistic effects conferring improved tolerance more effectively even severe freezing.

Authors+Show Affiliations

Postharvest Research Team, Department of Horticultural Crop Research, National Institute of Horticultural & Herbal Science, Rural Development Administration, Suwon, 440-706, Korea.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

24170089

Citation

Shin, Sun-Young, et al. "Co-expression of Monodehydroascorbate Reductase and Dehydroascorbate Reductase From Brassica Rapa Effectively Confers Tolerance to Freezing-induced Oxidative Stress." Molecules and Cells, vol. 36, no. 4, 2013, pp. 304-15.
Shin SY, Kim MH, Kim YH, et al. Co-expression of monodehydroascorbate reductase and dehydroascorbate reductase from Brassica rapa effectively confers tolerance to freezing-induced oxidative stress. Mol Cells. 2013;36(4):304-15.
Shin, S. Y., Kim, M. H., Kim, Y. H., Park, H. M., & Yoon, H. S. (2013). Co-expression of monodehydroascorbate reductase and dehydroascorbate reductase from Brassica rapa effectively confers tolerance to freezing-induced oxidative stress. Molecules and Cells, 36(4), 304-15. https://doi.org/10.1007/s10059-013-0071-4
Shin SY, et al. Co-expression of Monodehydroascorbate Reductase and Dehydroascorbate Reductase From Brassica Rapa Effectively Confers Tolerance to Freezing-induced Oxidative Stress. Mol Cells. 2013;36(4):304-15. PubMed PMID: 24170089.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Co-expression of monodehydroascorbate reductase and dehydroascorbate reductase from Brassica rapa effectively confers tolerance to freezing-induced oxidative stress. AU - Shin,Sun-Young, AU - Kim,Myung-Hee, AU - Kim,Yul-Ho, AU - Park,Hyang-Mi, AU - Yoon,Ho-Sung, Y1 - 2013/10/22/ PY - 2013/02/27/received PY - 2013/08/26/accepted PY - 2013/08/20/revised PY - 2013/10/31/entrez PY - 2013/10/31/pubmed PY - 2014/6/6/medline SP - 304 EP - 15 JF - Molecules and cells JO - Mol Cells VL - 36 IS - 4 N2 - Plants are exposed to various environmental stresses and have therefore developed antioxidant enzymes and molecules to protect their cellular components against toxicity derived from reactive oxygen species (ROS). Ascorbate is a very important antioxidant molecule in plants, and monodehydroascorbate reductase (MDHAR; EC 1.6.5.4) and dehydroascorbate reductase (DHAR; EC 1.8.5.1) are essential to regeneration of ascorbate for maintenance of ROS scavenging ability. The MDHAR and DHAR genes from Brassica rapa were cloned, transgenic plants overexpressing either BrMDHAR and BrDHAR were established, and then, each transgenic plant was hybridized to examine the effects of co-expression of both genes conferring tolerance to freezing. Transgenic plants co-overexpressing BrMDHAR and BrDHAR showed activated expression of relative antioxidant enzymes, and enhanced levels of glutathione and phenolics under freezing condition. Then, these alteration caused by co-expression led to alleviated redox status and lipid peroxidation and consequently conferred improved tolerance against severe freezing stress compared to transgenic plants overexpressing single gene. The results of this study suggested that although each expression of BrMDHAR or BrDHAR was available to according tolerance to freezing, the simultaneous expression of two genes generated synergistic effects conferring improved tolerance more effectively even severe freezing. SN - 0219-1032 UR - https://www.unboundmedicine.com/medline/citation/24170089/Co_expression_of_monodehydroascorbate_reductase_and_dehydroascorbate_reductase_from_Brassica_rapa_effectively_confers_tolerance_to_freezing_induced_oxidative_stress_ L2 - http://www.molcells.org/journal/view.html?year=2013&volume=36&number=4&spage=304 DB - PRIME DP - Unbound Medicine ER -