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Label-free surface-enhanced Raman spectroscopy for sensitive DNA detection by DNA-mediated silver nanoparticle growth.
Anal Chem. 2013 Dec 17; 85(24):11788-93.AC

Abstract

This work designed a label-free strategy for surface-enhanced Raman spectroscopic (SERS) detection of target DNA on a peptide nucleic acid (PNA) modified glass slide. Upon hybridization of the PNA with target DNA, the surface became negatively charged and allowed the absorption of silver ions on the DNA skeleton. After chemical reduction by hydroquinone, the formed silver nanoparticles could be further grown with a silver enhancement step to amplify the detectable SERS signal by absorbing rhodamine 6G as a SERS reporter on a silver nanoparticle surface. The growth of silver nanoparticles was characterized with X-ray photoelectron spectroscopy and a scanning electron microscopic image. The label-free SERS method achieved the detection of DNA with a linear range from 1.0 × 10(-10) to 1.0 × 10(-6) M and a detection limit of 45 pM. Through introducing a hybridization chain reaction to increase the DNA length, the Raman signal was further amplified, leading to a detection limit of 3.4 pM. This approach could discriminate perfectly matched target DNA from single-base mismatch DNA. This strategy possessed good capacity to integrate other amplification techniques for sensitive, high-throughput detection of genes.

Authors+Show Affiliations

State Key Laboratory of Analytical Chemistry for Life Science, School of Chemistry and Chemical Engineering, Nanjing University , Nanjing 210093, P. R. China.No affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

24171654

Citation

Gao, Fenglei, et al. "Label-free Surface-enhanced Raman Spectroscopy for Sensitive DNA Detection By DNA-mediated Silver Nanoparticle Growth." Analytical Chemistry, vol. 85, no. 24, 2013, pp. 11788-93.
Gao F, Lei J, Ju H. Label-free surface-enhanced Raman spectroscopy for sensitive DNA detection by DNA-mediated silver nanoparticle growth. Anal Chem. 2013;85(24):11788-93.
Gao, F., Lei, J., & Ju, H. (2013). Label-free surface-enhanced Raman spectroscopy for sensitive DNA detection by DNA-mediated silver nanoparticle growth. Analytical Chemistry, 85(24), 11788-93. https://doi.org/10.1021/ac4032109
Gao F, Lei J, Ju H. Label-free Surface-enhanced Raman Spectroscopy for Sensitive DNA Detection By DNA-mediated Silver Nanoparticle Growth. Anal Chem. 2013 Dec 17;85(24):11788-93. PubMed PMID: 24171654.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Label-free surface-enhanced Raman spectroscopy for sensitive DNA detection by DNA-mediated silver nanoparticle growth. AU - Gao,Fenglei, AU - Lei,Jianping, AU - Ju,Huangxian, Y1 - 2013/11/25/ PY - 2013/11/1/entrez PY - 2013/11/1/pubmed PY - 2014/7/16/medline SP - 11788 EP - 93 JF - Analytical chemistry JO - Anal Chem VL - 85 IS - 24 N2 - This work designed a label-free strategy for surface-enhanced Raman spectroscopic (SERS) detection of target DNA on a peptide nucleic acid (PNA) modified glass slide. Upon hybridization of the PNA with target DNA, the surface became negatively charged and allowed the absorption of silver ions on the DNA skeleton. After chemical reduction by hydroquinone, the formed silver nanoparticles could be further grown with a silver enhancement step to amplify the detectable SERS signal by absorbing rhodamine 6G as a SERS reporter on a silver nanoparticle surface. The growth of silver nanoparticles was characterized with X-ray photoelectron spectroscopy and a scanning electron microscopic image. The label-free SERS method achieved the detection of DNA with a linear range from 1.0 × 10(-10) to 1.0 × 10(-6) M and a detection limit of 45 pM. Through introducing a hybridization chain reaction to increase the DNA length, the Raman signal was further amplified, leading to a detection limit of 3.4 pM. This approach could discriminate perfectly matched target DNA from single-base mismatch DNA. This strategy possessed good capacity to integrate other amplification techniques for sensitive, high-throughput detection of genes. SN - 1520-6882 UR - https://www.unboundmedicine.com/medline/citation/24171654/Label_free_surface_enhanced_Raman_spectroscopy_for_sensitive_DNA_detection_by_DNA_mediated_silver_nanoparticle_growth_ L2 - https://doi.org/10.1021/ac4032109 DB - PRIME DP - Unbound Medicine ER -