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Platinum porous nanoparticles hybrid with metal ions as probes for simultaneous detection of multiplex cancer biomarkers.
Biosens Bioelectron. 2014 Mar 15; 53:324-9.BB

Abstract

In this work, platinum porous nanoparticles (PtPNPs) absorbed metal ions as electrochemical signals were fabricated. Clean-surface PtPNPs were prepared by a surfactant-free method and decorated with amino groups via 2-aminoethanethiol. Amino capped PtPNPs complexation with Cd(2+) and Cu(2+) to form PtPNPs-Cd(2+) and PtPNPs-Cu(2+) hybrids, respectively. Anti-CEA and Anti-AFP separately labeled with PtPNPs-Cd(2+) and PtPNPs-Cu(2+) were used as distinguishable signal tags for capturing antigens. The metal ions were detected in a single run through differential pulse voltammetry (DPV) without acid dissolution, electric potentials and peak heights of which reflected the identity and concentrations of the corresponding antigen. Ionic liquid reduced graphene oxide (IL-rGO) modified glassy carbon electrode (GCE) was used as a substrate, which was rich in amino groups to immobilize antibodies by glutaraldehyde through cross-link between aldehyde groups and amino groups. Using the proposed probes and platform, a novel sandwich-type electrochemical immunosensor for simultaneous detecting carcinoembryonic antigen (CEA) and alpha-fetoprotein (AFP) was successfully developed. This immunoassay possessed good linearity from 0.05 ng mL(-1) to 200 ng mL(-1) for both CEA and AFP. The detection limit of CEA was 0.002 ng mL(-1) and that of AFP was 0.05 ng mL(-1) (S/N=3). Furthermore, analysis of clinical serum samples using this immunosensor was well consistent with the data determined by the enzyme-linked immunosorbent assay (ELISA). It suggested that the proposed electrochemical immunoassay provided a potential application of clinical screening for early-stage cancers.

Authors+Show Affiliations

Department of Chemistry, Capital Normal University, Beijing 100048, China.No affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

24176967

Citation

Wang, Zifeng, et al. "Platinum Porous Nanoparticles Hybrid With Metal Ions as Probes for Simultaneous Detection of Multiplex Cancer Biomarkers." Biosensors & Bioelectronics, vol. 53, 2014, pp. 324-9.
Wang Z, Liu N, Ma Z. Platinum porous nanoparticles hybrid with metal ions as probes for simultaneous detection of multiplex cancer biomarkers. Biosens Bioelectron. 2014;53:324-9.
Wang, Z., Liu, N., & Ma, Z. (2014). Platinum porous nanoparticles hybrid with metal ions as probes for simultaneous detection of multiplex cancer biomarkers. Biosensors & Bioelectronics, 53, 324-9. https://doi.org/10.1016/j.bios.2013.10.009
Wang Z, Liu N, Ma Z. Platinum Porous Nanoparticles Hybrid With Metal Ions as Probes for Simultaneous Detection of Multiplex Cancer Biomarkers. Biosens Bioelectron. 2014 Mar 15;53:324-9. PubMed PMID: 24176967.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Platinum porous nanoparticles hybrid with metal ions as probes for simultaneous detection of multiplex cancer biomarkers. AU - Wang,Zifeng, AU - Liu,Na, AU - Ma,Zhanfang, Y1 - 2013/10/15/ PY - 2013/08/13/received PY - 2013/10/07/revised PY - 2013/10/07/accepted PY - 2013/11/2/entrez PY - 2013/11/2/pubmed PY - 2014/7/16/medline KW - Alpha-fetoprotein KW - Carcinoembryonic antigen KW - Electrochemical immunosensor KW - Ionic liquid functionalized reduced graphene oxide KW - Platinum porous nanoparticles SP - 324 EP - 9 JF - Biosensors & bioelectronics JO - Biosens Bioelectron VL - 53 N2 - In this work, platinum porous nanoparticles (PtPNPs) absorbed metal ions as electrochemical signals were fabricated. Clean-surface PtPNPs were prepared by a surfactant-free method and decorated with amino groups via 2-aminoethanethiol. Amino capped PtPNPs complexation with Cd(2+) and Cu(2+) to form PtPNPs-Cd(2+) and PtPNPs-Cu(2+) hybrids, respectively. Anti-CEA and Anti-AFP separately labeled with PtPNPs-Cd(2+) and PtPNPs-Cu(2+) were used as distinguishable signal tags for capturing antigens. The metal ions were detected in a single run through differential pulse voltammetry (DPV) without acid dissolution, electric potentials and peak heights of which reflected the identity and concentrations of the corresponding antigen. Ionic liquid reduced graphene oxide (IL-rGO) modified glassy carbon electrode (GCE) was used as a substrate, which was rich in amino groups to immobilize antibodies by glutaraldehyde through cross-link between aldehyde groups and amino groups. Using the proposed probes and platform, a novel sandwich-type electrochemical immunosensor for simultaneous detecting carcinoembryonic antigen (CEA) and alpha-fetoprotein (AFP) was successfully developed. This immunoassay possessed good linearity from 0.05 ng mL(-1) to 200 ng mL(-1) for both CEA and AFP. The detection limit of CEA was 0.002 ng mL(-1) and that of AFP was 0.05 ng mL(-1) (S/N=3). Furthermore, analysis of clinical serum samples using this immunosensor was well consistent with the data determined by the enzyme-linked immunosorbent assay (ELISA). It suggested that the proposed electrochemical immunoassay provided a potential application of clinical screening for early-stage cancers. SN - 1873-4235 UR - https://www.unboundmedicine.com/medline/citation/24176967/Platinum_porous_nanoparticles_hybrid_with_metal_ions_as_probes_for_simultaneous_detection_of_multiplex_cancer_biomarkers_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0956-5663(13)00701-X DB - PRIME DP - Unbound Medicine ER -