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Synthesis, characterization, DNA interaction, antioxidant and anticancer activity studies of ruthenium(II) polypyridyl complexes.
J Photochem Photobiol B. 2013 Dec 05; 129:48-56.JP

Abstract

Two new Ru(II) polypyridyl complexes [Ru(phen)₂(adppz)](ClO₄)₂ (1) and [Ru(dip)₂(adppz)](ClO₄)₂ (2) have been synthesized and characterized. The DNA-binding constants were determined to be 6.54 ± 0.42 × 10(5) and 7.65 ± 0.20 × 10(5)M(-1) for complexes 1 and 2. DNA binding experiments indicated that complexes 1 and 2 interact with DNA through intercalative mode. Antioxidant activity shows that the complexes have significant hydroxyl radical scavenging activity. Cytotoxic activities suggest that the complex 2 exhibits higher cytotoxic activity against BEL-7402, MG-63 and SKBR-3 cells than complex 1 under identical conditions. Complexes 1 and 2 can induce apoptosis of BEl-7402 cells. We have identified several cellular mechanisms induced by 1 and 2 in BEL-7402 cells, including the level detection of ROS, activation of procaspase 3, caspase 7, the expression of antiapoptotic proteins Bcl-x, Bcl-2, proapoptotic proteins Bad, Bax, Bid and cell cycle arrest. Thus, complexes 1 and 2 inhibit growth of BEL-7402 cells through induction of apoptotic cell death, enhancement of ROS levels and S-phase and G0/G1 cell cycle arrest. Further investigations have shown that complex 2 induces apoptosis by regulating the expression of Bcl-2 family proteins.

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Authors+Show Affiliations

Jiang GB
School of Pharmacy, Guangdong Pharmaceutical University, Guangzhou 510006, PR China.
Xie YY
No affiliation info available
Lin GJ
No affiliation info available
Huang HL
No affiliation info available
Liang ZH
No affiliation info available
Liu YJ
No affiliation info available

MeSH

Antineoplastic AgentsAntioxidantsApoptosisCaspase 3Caspase 7Cell Cycle CheckpointsCell Line, TumorCell ProliferationCoordination ComplexesDNAHumansProto-Oncogene Proteins c-bcl-2Reactive Oxygen SpeciesRuthenium

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

24177204

Citation

Jiang, Guang-Bin, et al. "Synthesis, Characterization, DNA Interaction, Antioxidant and Anticancer Activity Studies of ruthenium(II) Polypyridyl Complexes." Journal of Photochemistry and Photobiology. B, Biology, vol. 129, 2013, pp. 48-56.
Jiang GB, Xie YY, Lin GJ, et al. Synthesis, characterization, DNA interaction, antioxidant and anticancer activity studies of ruthenium(II) polypyridyl complexes. J Photochem Photobiol B. 2013;129:48-56.
Jiang, G. B., Xie, Y. Y., Lin, G. J., Huang, H. L., Liang, Z. H., & Liu, Y. J. (2013). Synthesis, characterization, DNA interaction, antioxidant and anticancer activity studies of ruthenium(II) polypyridyl complexes. Journal of Photochemistry and Photobiology. B, Biology, 129, 48-56. https://doi.org/10.1016/j.jphotobiol.2013.09.009
Jiang GB, et al. Synthesis, Characterization, DNA Interaction, Antioxidant and Anticancer Activity Studies of ruthenium(II) Polypyridyl Complexes. J Photochem Photobiol B. 2013 Dec 5;129:48-56. PubMed PMID: 24177204.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Synthesis, characterization, DNA interaction, antioxidant and anticancer activity studies of ruthenium(II) polypyridyl complexes. AU - Jiang,Guang-Bin, AU - Xie,Yang-Yin, AU - Lin,Gan-Jian, AU - Huang,Hong-Liang, AU - Liang,Zhen-Hua, AU - Liu,Yun-Jun, Y1 - 2013/10/15/ PY - 2013/08/12/received PY - 2013/09/26/revised PY - 2013/09/27/accepted PY - 2013/11/2/entrez PY - 2013/11/2/pubmed PY - 2014/7/16/medline KW - Cell cycle arrest KW - Cytotoxicity in vitro KW - DNA-binding KW - Reactive oxygen species KW - Ru(II) polypyridyl complex KW - Western blot SP - 48 EP - 56 JF - Journal of photochemistry and photobiology. B, Biology JO - J Photochem Photobiol B VL - 129 N2 - Two new Ru(II) polypyridyl complexes [Ru(phen)₂(adppz)](ClO₄)₂ (1) and [Ru(dip)₂(adppz)](ClO₄)₂ (2) have been synthesized and characterized. The DNA-binding constants were determined to be 6.54 ± 0.42 × 10(5) and 7.65 ± 0.20 × 10(5)M(-1) for complexes 1 and 2. DNA binding experiments indicated that complexes 1 and 2 interact with DNA through intercalative mode. Antioxidant activity shows that the complexes have significant hydroxyl radical scavenging activity. Cytotoxic activities suggest that the complex 2 exhibits higher cytotoxic activity against BEL-7402, MG-63 and SKBR-3 cells than complex 1 under identical conditions. Complexes 1 and 2 can induce apoptosis of BEl-7402 cells. We have identified several cellular mechanisms induced by 1 and 2 in BEL-7402 cells, including the level detection of ROS, activation of procaspase 3, caspase 7, the expression of antiapoptotic proteins Bcl-x, Bcl-2, proapoptotic proteins Bad, Bax, Bid and cell cycle arrest. Thus, complexes 1 and 2 inhibit growth of BEL-7402 cells through induction of apoptotic cell death, enhancement of ROS levels and S-phase and G0/G1 cell cycle arrest. Further investigations have shown that complex 2 induces apoptosis by regulating the expression of Bcl-2 family proteins. SN - 1873-2682 UR - https://www.unboundmedicine.com/medline/citation/24177204/Synthesis_characterization_DNA_interaction_antioxidant_and_anticancer_activity_studies_of_ruthenium_II__polypyridyl_complexes_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S1011-1344(13)00217-0 DB - PRIME DP - Unbound Medicine ER -