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Conservation of lipid metabolic gene transcriptional regulatory networks in fish and mammals.
Gene. 2014 Jan 15; 534(1):1-9.GENE

Abstract

Lipid content and composition in aquafeeds have changed rapidly as a result of the recent drive to replace ecologically limited marine ingredients, fishmeal and fish oil (FO). Terrestrial plant products are the most economic and sustainable alternative; however, plant meals and oils are devoid of physiologically important cholesterol and long-chain polyunsaturated fatty acids (LC-PUFA), eicosapentaenoic (EPA), docosahexaenoic (DHA) and arachidonic (ARA) acids. Although replacement of dietary FO with vegetable oil (VO) has little effect on growth in Atlantic salmon (Salmo salar), several studies have shown major effects on the activity and expression of genes involved in lipid homeostasis. In vertebrates, sterols and LC-PUFA play crucial roles in lipid metabolism by direct interaction with lipid-sensing transcription factors (TFs) and consequent regulation of target genes. The primary aim of the present study was to elucidate the role of key TFs in the transcriptional regulation of lipid metabolism in fish by transfection and overexpression of TFs. The results show that the expression of genes of LC-PUFA biosynthesis (elovl and fads2) and cholesterol metabolism (abca1) are regulated by Lxr and Srebp TFs in salmon, indicating highly conserved regulatory mechanism across vertebrates. In addition, srebp1 and srebp2 mRNA respond to replacement of dietary FO with VO. Thus, Atlantic salmon adjust lipid metabolism in response to dietary lipid composition through the transcriptional regulation of gene expression. It may be possible to further increase efficient and effective use of sustainable alternatives to marine products in aquaculture by considering these important molecular interactions when formulating diets.

Authors+Show Affiliations

School of Natural Sciences, University of Stirling, Institute of Aquaculture, Stirling FK9 4LA, UK. Electronic address: g.e.carmonaantonanzas@stir.ac.uk.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

24177230

Citation

Carmona-Antoñanzas, Greta, et al. "Conservation of Lipid Metabolic Gene Transcriptional Regulatory Networks in Fish and Mammals." Gene, vol. 534, no. 1, 2014, pp. 1-9.
Carmona-Antoñanzas G, Tocher DR, Martinez-Rubio L, et al. Conservation of lipid metabolic gene transcriptional regulatory networks in fish and mammals. Gene. 2014;534(1):1-9.
Carmona-Antoñanzas, G., Tocher, D. R., Martinez-Rubio, L., & Leaver, M. J. (2014). Conservation of lipid metabolic gene transcriptional regulatory networks in fish and mammals. Gene, 534(1), 1-9. https://doi.org/10.1016/j.gene.2013.10.040
Carmona-Antoñanzas G, et al. Conservation of Lipid Metabolic Gene Transcriptional Regulatory Networks in Fish and Mammals. Gene. 2014 Jan 15;534(1):1-9. PubMed PMID: 24177230.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Conservation of lipid metabolic gene transcriptional regulatory networks in fish and mammals. AU - Carmona-Antoñanzas,Greta, AU - Tocher,Douglas R, AU - Martinez-Rubio,Laura, AU - Leaver,Michael J, Y1 - 2013/10/28/ PY - 2013/07/29/received PY - 2013/10/01/revised PY - 2013/10/21/accepted PY - 2013/11/2/entrez PY - 2013/11/2/pubmed PY - 2014/1/18/medline KW - 3-hydroxy-3-methyl-glutaryl-CoA reductase KW - ABCA1 KW - ACOX KW - ARA KW - ATP-binding cassette transporter 1 KW - Atlantic salmon KW - CYP7α1 KW - DHA KW - DMEM KW - DNA complementary to RNA KW - Dulbecco's modified eagle medium KW - EDTA KW - ELF-1α KW - ELOVL KW - EPA KW - ER KW - FA KW - FADS KW - FAS KW - FBS KW - FHM KW - FO KW - Fatty acid KW - Gene expression KW - HMG-CoAR KW - LBD KW - LC-PUFA KW - LO KW - LXR KW - LXR response element KW - LXRE KW - Lipid KW - ORF KW - PBS KW - PPAR KW - PPAR response element KW - PPRE KW - PUFA KW - Pyloric caeca KW - RE KW - RO KW - RXR KW - SCD KW - SHK-1 KW - SO KW - SRE KW - SREBP KW - TF KW - Transcription factor KW - UAS KW - VO KW - aa KW - acyl-CoA oxidase KW - amino acid(s) KW - arachidonic acid KW - base pair(s) KW - bp KW - cDNA KW - cholesterol 7alpha-hydroxylase KW - docosahexaenoic acid KW - eicosapentaenoic acid KW - elongase of very long-chain fatty acids KW - elongation factor 1 alpha KW - endoplasmic reticulum KW - ethylenediaminetetraacetic acid KW - fathead minnow epithelial cells KW - fatty acid KW - fatty acid desaturase KW - fatty acid synthase KW - fetal bovine serum KW - fish oil KW - ligand binding domain KW - linseed oil KW - liver X receptor KW - long-chain polyunsaturated fatty acids KW - mRNA KW - messenger RNA KW - open reading frame KW - peroxisome proliferator-activated receptors KW - phosphate buffer saline KW - polyunsaturated fatty acids KW - qPCR KW - quantitative RT-PCR KW - rapeseed oil KW - response element KW - retinoid X receptor KW - salmon head kidney cells KW - serum response element KW - soybean oil KW - stearoyl CoA desaturase KW - sterol regulatory element binding proteins KW - transcription factor KW - upstream activation sequence KW - vegetable oil SP - 1 EP - 9 JF - Gene JO - Gene VL - 534 IS - 1 N2 - Lipid content and composition in aquafeeds have changed rapidly as a result of the recent drive to replace ecologically limited marine ingredients, fishmeal and fish oil (FO). Terrestrial plant products are the most economic and sustainable alternative; however, plant meals and oils are devoid of physiologically important cholesterol and long-chain polyunsaturated fatty acids (LC-PUFA), eicosapentaenoic (EPA), docosahexaenoic (DHA) and arachidonic (ARA) acids. Although replacement of dietary FO with vegetable oil (VO) has little effect on growth in Atlantic salmon (Salmo salar), several studies have shown major effects on the activity and expression of genes involved in lipid homeostasis. In vertebrates, sterols and LC-PUFA play crucial roles in lipid metabolism by direct interaction with lipid-sensing transcription factors (TFs) and consequent regulation of target genes. The primary aim of the present study was to elucidate the role of key TFs in the transcriptional regulation of lipid metabolism in fish by transfection and overexpression of TFs. The results show that the expression of genes of LC-PUFA biosynthesis (elovl and fads2) and cholesterol metabolism (abca1) are regulated by Lxr and Srebp TFs in salmon, indicating highly conserved regulatory mechanism across vertebrates. In addition, srebp1 and srebp2 mRNA respond to replacement of dietary FO with VO. Thus, Atlantic salmon adjust lipid metabolism in response to dietary lipid composition through the transcriptional regulation of gene expression. It may be possible to further increase efficient and effective use of sustainable alternatives to marine products in aquaculture by considering these important molecular interactions when formulating diets. SN - 1879-0038 UR - https://www.unboundmedicine.com/medline/citation/24177230/Conservation_of_lipid_metabolic_gene_transcriptional_regulatory_networks_in_fish_and_mammals_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0378-1119(13)01449-2 DB - PRIME DP - Unbound Medicine ER -